Tissue culture and rapid propagation method of succulent plants

A technology for rapid propagation of succulents and tissue culture, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of low reproduction coefficient and long reproduction cycle, achieve high multiplication frequency, short production cycle, improve The effect of reproduction coefficient and reproduction speed

Pending Publication Date: 2020-04-28
云南爱花多肉花卉有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the deficiencies of the prior art, the present invention provides a method for tissue culture and rapid propagation of succulents with reliable performance, which solves the problems of low reproductive coefficient and long propagation cycle of traditional succulents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The tissue culture rapid propagation method of succulents is characterized in that: comprising the following steps:

[0023] S1) Explant selection and disinfection:

[0024] Use the leaves or stems of succulent plants as explants, put the explants in a beaker and rinse them with tap water. First, wash them with an appropriate amount of detergent, then rinse them under tap water for more than 3 minutes, and then use them on an ultra-clean workbench. Soak in 75% alcohol for 30 seconds, rinse with sterile water for 2 to 3 times, quickly pour into 0.1% mercuric chloride solution and soak for 5 minutes, take out the explants, rinse with sterile water for 4 to 5 times, and blot dry with filter paper Obtain sterile explants after water;

[0025] S2) Primary induction culture:

[0026] Under sterile conditions, the obtained explants were cut into small slices of 1 to 2 cm in length. The small slices were soaked in 75% alcohol and washed with water, and then inoculated in the ...

Embodiment 2

[0034] The tissue culture rapid propagation method of succulents is characterized in that: comprising the following steps:

[0035] S1) Explant selection and disinfection:

[0036] Use the leaves or stems of succulent plants as explants, put the explants in a beaker and rinse them with tap water. First, wash them with an appropriate amount of detergent, then rinse them under tap water for more than 3 minutes, and then use them on an ultra-clean workbench. Soak in 75% alcohol for 30 seconds, rinse with sterile water for 2 to 3 times, quickly pour into 0.1% mercuric chloride solution and soak for 5 minutes, take out the explants, rinse with sterile water for 4 to 5 times, and blot dry with filter paper Obtain sterile explants after water;

[0037] S2) Primary induction culture:

[0038] Under sterile conditions, the obtained explants were cut into small slices of 1 to 2 cm in length. The small slices were soaked in 75% alcohol and washed with water, and then inoculated in the ...

Embodiment 3

[0046] The tissue culture rapid propagation method of succulents is characterized in that: comprising the following steps:

[0047] S1) Explant selection and disinfection:

[0048] Use the leaves or stems of succulent plants as explants, put the explants in a beaker and rinse them with tap water. First, wash them with an appropriate amount of detergent, then rinse them under tap water for more than 3 minutes, and then use them on an ultra-clean workbench. Soak in 75% alcohol for 30 seconds, rinse with sterile water for 2 to 3 times, quickly pour into 0.1% mercuric chloride solution and soak for 5 minutes, take out the explants, rinse with sterile water for 4 to 5 times, and blot dry with filter paper Obtain sterile explants after water;

[0049] S2) Primary induction culture:

[0050] Under sterile conditions, the obtained explants were cut into small slices of 1 to 2 cm in length. The small slices were soaked in 75% alcohol and washed with water, and then inoculated in the ...

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PUM

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Abstract

The invention relates to the technical field of succulent plant planting, in particular to a tissue culture and rapid propagation method of succulent plants. The extraction method comprises the following steps: S1, explant selection and disinfection; S2, primary induction culture; S3, multiplication culture; S4, induced rooting culture; and S5, seedling hardening and transplanting. Meanwhile, theinvention further discloses components and proportions of a primary induction culture medium, a multiplication culture medium and a rooting culture medium. The succulent plant seedlings obtained through the tissue culture and rapid propagation method are short in production period, high in multiplication frequency and neat, the propagation coefficient and propagation speed of the succulent plantsare increased, the germination rate and rooting rate of the succulent plants are about three times that of a conventional culture medium, the strain advantages of the succulent plants can be well kept, a large number of tissue culture seedlings suitable for transplanting can be rapidly propagated, the grown seedling plants are consistent in shape, the method is easy to operate, and industrial production is facilitated.

Description

technical field [0001] The invention relates to the technical field of planting succulents, in particular to a method for tissue culture and rapid propagation of succulents. Background technique [0002] Succulentsrefers to a certain part of the plant's vegetative organs, a type of plant that has developed parenchyma to store water, and appears plump and succulent in appearance. In gardening, it is also called succulent plant or succulent flower, but the name succulent plant is the most commonly used. In recent years, with the help of the vigorous publicity of the Internet and the low starting point of maintenance of succulents, relatively obvious and stable appearance, colorful colors, and many varieties, succulents have been known and loved by the public in a relatively short period of time, and then began to grow. It is popular all over the country. At present, most of the propagation techniques of succulents use leaf cuttings for propagation, which has low efficiency, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001A01H4/005
Inventor 周杰
Owner 云南爱花多肉花卉有限公司
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