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Paecilomyces lilacinus and application

The technology of Paecilomyces lilacinus and inoculum is applied to the application field of Paecilomyces lilacinus and its inoculants for preventing and controlling plant root-knot nematode diseases, and can solve the problems of complicated preparation process, lack of preparations, and shelf life of biocontrol preparations. short and so on

Active Publication Date: 2009-04-29
中农绿康(北京)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. Most of the existing biocontrol agents for the prevention and treatment of plant nematode diseases are spore preparations, which have high requirements for the production of upstream active ingredients, and often require two-step fermentation (liquid fermentation combined with solid fermentation) to obtain the active ingredient, fungal spores, and the preparation process is very cumbersome
[0006] 2. The level of dosage form is low. At present, most of the biocontrol agents for nematode control are powders, suspension concentrates, etc., with extensive technology and poor soil applicability, which is not conducive to soil environment application, and it is often difficult to overcome the unfavorable environmental factors that inhibit germination in soil. Form a colony, and these preparations mostly appear in the form of seed coating or seed dressing, and the amount of bacteria carried is very limited
Therefore, the lack of market competitiveness limits its application
[0007] 3. The stability is poor, and the activity of the bacterial agent loses very quickly, resulting in a short shelf life of biocontrol agents, making it difficult to produce commercially
In order to ensure that the preparation has a long shelf life, strict storage conditions are often required, which directly limits the large-scale application of fungal biopesticides
[0008] To sum up, although the biological control pesticides for plant nematode diseases in the soil have a very broad market space, some strains with certain biocontrol potential have been obtained through the research on the biological control of plant parasitic nematodes for a long time, but their industrial application is still limited. The progress is slow, and there is a lack of preparations that can meet the needs of nematode biocontrol in actual production

Method used

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  • Paecilomyces lilacinus and application
  • Paecilomyces lilacinus and application
  • Paecilomyces lilacinus and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1. Isolation and preservation of Paecilomyces lilacinus (P.lilacinus) FZ07-9F-5 CGMCC No.2780

[0059] Collect roots of tobacco root-knot nematode disease in Tadian, Eshan, Yunnan, wash the diseased roots with tap water for 3-5 minutes to remove the soil, then put the diseased roots under a dissecting microscope, pick out the oocysts with tweezers, and treat the oocysts with 1% sodium hypochlorite for 1 minute Disinfect the surface of oocysts and release nematode eggs, pass the sodium hypochlorite egg suspension through 200 μm and 30 μm mesh sieves respectively, rinse 3 times with sterilized distilled water to remove sodium hypochlorite, spread approximately every 100 eggs on a PDA plate, and observe the colonies every day The situation arises. Finally, a nematicidal fungal strain was isolated from the eggs of root-knot nematode, named FZ07-9F-5, which was deposited in the China Microorganism Culture Collection located in Datun Road, Chaoyang District, Beijing, ...

Embodiment 2

[0060] Embodiment 2, the cultivation of Paecilomyces lilacinus (P.lilacinus) FZ07-9F-5 CGMCC No.2780 and the preparation of its bacterial agent

[0061] 1. Cultivation of Paecilomyces lilacinus (P.lilacinus) FZ07-9F-5 CGMCC No.2780

[0062] 1. Test tube culture

[0063] Preparation of potato agar medium: 200g potatoes, 20g glucose, 16g agar, dilute to 1L with water, pH6-7.

[0064] Paecilomyces lilacinus (P.lilacinus) FZ07-9F-5 CGMCC No.2780 was inoculated on the potato agar medium in the test tube, and cultivated at 25°C for 72-96h to obtain activated Paecilomyces lilacinus ( P. lilacinus) FZ07-9F-5 CGMCC No.2780.

[0065] 2. Expand training

[0066] Preparation of Paecilomyces lilacinus (P.lilacinus) FZ07-9F-5 CGMCC No.2780 special liquid medium: maltose (C source) 30g, yeast (N source) 6.12g, K 2 HPO 4 1g, MgSO 4 0.5g, KCl 0.5g, FeSO 4 0.01g, ZnSO 4 ·7H 2 O 10mg, dilute to 1L with water, pH6-7.

[0067] Paecilomyces lilacinus (P.lilacinus) FZ07-9F-5 CGMCC No.278...

Embodiment 3

[0076] Embodiment 3, the cultivation of Paecilomyces lilacinus (P.lilacinus) FZ07-9F-5CGMCC No.2780 and the preparation of its bacterial agent

[0077] 1. Cultivation of Paecilomyces lilacinus (P.lilacinus) FZ07-9F-5 CGMCC No.2780

[0078] 1. Test tube culture

[0079] Preparation of potato agar medium: 200g potatoes, 20g glucose, 16g agar, dilute to 1L with water, pH6-7.

[0080] Paecilomyces lilacinus (P.lilacinus) FZ07-9F-5 CGMCC No.2780 was inoculated on the potato agar medium in a test tube, and cultured at 25°C for 72 hours to obtain activated Paecilomyces lilacinus (P. lilacinus) FZ07-9F-5CGMCC No.2780.

[0081] 2. Expand training

[0082] Preparation of special liquid medium for Paecilomyces lilacinus (P.lilacinus) FZ07-9F-5 CGMCC No.2780: maltose 20g, yeast 5g, K 2 HPO 4 0.5g, MgSO 4 0.1g, KCl 0.1g, FeSO 4 0.001g, ZnSO 4 ·7H 2 O5mg, dilute to 1L with water, pH6-7.

[0083] Inoculate Paecilomyces lilacinus (P.lilacinus) FZ07-9F-5 CGMCC No.2780 through step ...

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Abstract

The invention discloses Paecilomyces lilacinus FZ07-9F-5 CGMCC No.2780, microbial inoculum and application thereof. The microbial inoculum is wireworm killing biological microbial inoculum with slow application function, which is prepared by the following steps: using a macromolecular material, namely sodium alginate as basic skeleton, using mycelium and spore mixture of P. lilacinus FZ07-9F-5 CGMCC No.2780 as active components, using infusorial earth as a carrier, and adopting a method of sodium alginate-calcium chloride reaction according to the characteristic of forming gel by the sodium alginate and polyvalent cations to be prepared into the microbial inoculum. Proved by experiments, the microbial inoculum has better wireworm killing efficacy, has control ratio up to between 50 and 60 percent, is comparatively stable, has no noxious chemical residues (innocuous and non-residue), does not pollute agricultural products and environment (does not react with other substances in the environment), and has the characteristics of high cost performance and convenient application. The microbial inoculum plays an important role in control of wireworm diseases of plant roots and knots, and has wide application prospect.

Description

technical field [0001] The invention relates to a strain of Paecilomyces lilacinus and its application, in particular to a strain of Paecilomyces lilacinus and its agent and the application of the agent in preventing and treating plant root-knot nematode diseases. Background technique [0002] Among the four major pathogens that cause plant infectious diseases, plant parasitic nematodes are more harmful than bacteria and viruses, and are second only to fungal diseases. Plant parasitic nematodes cause about 100 billion US dollars of losses in world agricultural production every year, among which root-knot nematodes (Meloidogyne spp.) are the most harmful type of plant parasitic nematodes, and the economic losses caused by the world's important economic crops are as high as tens of millions of dollars each year. tens of billions of dollars. Within the genus Meloidogyne, M. incognita, M. arenaria, M. javanica and M. hapla The root-knot nematodes of the four species are the mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/79
Inventor 段维军刘杏忠谢红艳
Owner 中农绿康(北京)生物技术有限公司
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