Artificial culturing method for cicade flower

A technology of artificial cultivation and cicadae, which is applied in the biological field and can solve problems such as unsatisfactory production

Inactive Publication Date: 2008-07-23
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its main production areas are in Jiangsu, Zhejiang, Sichuan, Yun, Gui, Fu, and Guangxi, but the output is far from meeting people's demand for cicadae as medicine and health food

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] From the Paecilomyces cicadae strains collected from the nature reserve, the original strains with fast growth, dense and plump colonies, and obvious angular changes (mycelia, spores, dense or sparse) in the later stage of culture were selected, and the microscopic The heterokaryon strain of Paecilomyces cicadae was observed to have the typical spore-forming structure in its angular changes; the heterokaryon strain of Paecilomyces cicadae was placed on the Chase plate at 25°C for 14 days, and the diameter of the colony was 55mm, and the hyphae were thin and white. , flat, soybean juice yellow on the back; conidiophores grow on aerial hyphae, 3.6 μm wide, whorls composed of 2-5 bottle-shaped stalks; bottle-shaped stalks are 5.4-7.2×1.2-2.4 in length μm, consisting of a spherical enlarged base and a tapered apex about 0.5 μm wide; conidia oblong to cylindrical, 4.8-6.0 × 1.8-3.0 μm. On the Sabouraud plate, the diameter of the colony is 70mm at 25°C for 14 days, white fluf...

Embodiment 2

[0018] From the Paecilomyces cicadae strains collected from the nature reserve, select the plates with faster growth, dense and plump colonies, and obvious angular separation, and purely isolate the spore-type and mycelial-type strains and store them in the refrigerator at 4 °C , mixed inoculation with the heterokaryon bacterial strain obtained by mating during use, after cultivating Paecilomyces cicadae for 1 week with Cha's (Capek) medium, adopt 0.05% Tween 80 water to remove Paecilomyces cicadae spores from Wash the conidia on the slant of the petri dish or test tube and dilute to 1×10 6 The Paecilomyces cicadae spore liquid of the conidia / ml concentration will infect the 5th instar silkworm, put it into the Paecilomyces cicadae spore liquid gently for 2 to 3 seconds and pick it up, put it into a sterilized petri dish, Cicada flowers were obtained by culturing at room temperature at 20°C-28°C for 2 to 3 weeks.

Embodiment 3

[0020] From the Paecilomyces cicadae strains collected from the nature reserve, the original strains with fast growth, dense and plump colonies, and obvious angular changes (mycelia, spores, dense or sparse) in the later stage of culture were selected, and the microscopic The heterokaryon strain of Paecilomyces cicadae was observed to have the typical spore-forming structure in its angular changes; the heterokaryon strain of Paecilomyces cicadae was placed on the Chase plate at 25°C for 14 days, and the diameter of the colony was 55mm, and the hyphae were thin and white. , flat, soybean juice yellow on the back; conidiophores grow on aerial hyphae, 3.6 μm wide, whorls composed of 2-5 bottle-shaped stalks; bottle-shaped stalks are 5.4-7.2×1.2-2.4 in length μm, consisting of a spherical enlarged base and a tapered apex about 0.5 μm wide; conidia oblong to cylindrical, 4.8-6.0 × 1.8-3.0 μm. On the Sabouraud plate, the diameter of the colony is 70mm at 25°C for 14 days, white fluf...

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Abstract

The invention discloses a culturing method of artificially cicada fungus, which comprises the following steps: (1) select original bacterial strains with fast growth speed, compact and chubby bacterial colonies and obvious angle distortion and separation in later culturing phase from the paecilomyces cicada bacterial strains in the nature reserves; the bacterial strains with the typical conidial production structure when observing the angle distortion using a microscope is the paecilomyces cicada heterokaryotic bacterial strains; (2) after culturing the paecilomyces cicada of the paecilomyces cicada heterokaryotic bacterial strains in culture media for a week, use the 0.05% tweenum 80 water to separate the conidia from the paecilomyces cicada spores in the inclined plane of the test tubes or culture dishes, and then dilute the conidia into the paecilomyces cicada spore liquid with the concentration being 1*10<6>-1*10<7> conidia / ml; put the infection bodies in the paecilomyces cicada spore liquid for infection and culture for two to three weeks with normal temperature of 20 to 28 DEG C to get cicada fungus. The culturing method of artificially cicada fungus has the advantages that: the method is simple and practical; infection bodies the same as the cicada fungus collected in natural mountain forests can be gotten; the method is endowed with market popularization value.

Description

technical field [0001] The invention belongs to the biological field, and in particular relates to a method for artificially cultivating cicada flowers by using the entomopathogenic fungus Paecilomyces cicadae. Background technique [0002] Cicada is a complex of insects and fungi that parasitize the nymphs of Cicada cicadae, an anamorph of Paecilomycescicadae (Miquel) Samson (Samson 1974), because the spore bundles that grow out of its head resemble flower buds Hence the name cicada. Cicadae is an ancient, valuable and young insect-living fungus with great development value. In Chinese folk, cicadae is a precious herbal medicine for clearing away heat, detoxifying, dispelling wind, analgesic and improving eyesight; In recent years, it has been studied that the fermented mycelium of Paecilomyces cicadae contains a variety of active substances, such as: mannitol, alkaloids, ergosterol, proteins, amino acids, polysaccharides, adenosine, vitamin E, vitamin A, unsaturated fatt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/79
Inventor 刘爱英邹晓胡海燕罗力赵杰宏
Owner GUIZHOU UNIV
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