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Paecilomyces cicadae biological bacteria preparation, method for producing same and application of same to plant nematode prevention

A technology of Paecilomyces cicadae and a biological inoculum, which is applied in the control of plant nematodes, in the field of Paecilomyces cicadae biological inoculum and its preparation, and can solve the problems of short product shelf life, small application area, and unstable effect. , to achieve high drug resistance, eliminate yield loss, and facilitate large-scale production

Inactive Publication Date: 2009-09-02
刘伟平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to environmental protection requirements and food safety requirements, chemical nematicides are increasingly restricted in agricultural production. At present, various biocontrol nematicides have various defects and deficiencies, such as small application areas, The shelf life of the product is short, the effect is not stable enough, etc.

Method used

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  • Paecilomyces cicadae biological bacteria preparation, method for producing same and application of same to plant nematode prevention
  • Paecilomyces cicadae biological bacteria preparation, method for producing same and application of same to plant nematode prevention
  • Paecilomyces cicadae biological bacteria preparation, method for producing same and application of same to plant nematode prevention

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Embodiment 1

[0059] The Paecilomyces cicadae biological bacterial agent prepared by using biological bacteria according to the present invention adopts Paecilomyces cicadae to carry out strain culture, adopts liquid fermentation, after mass reproduction, directly processes or prepares biological nematicide bacterial agent by carrier adsorption method, commercial product The preparation is a mixture of Paecilomyces cicadae and its culture medium, auxiliary materials and carriers;

[0060] Described culture medium is the mixture of protein powder, silkworm chrysalis powder, willow bark powder, tea powder, water, and its parts by weight are as follows

[0061] Protein powder 100-300, silkworm chrysalis powder 100-300,

[0062] Willow bark powder 10-40, tea powder 5-10,

[0063] Water 1000-1500;

[0064] The auxiliary material is a mixture of protein powder and silkworm chrysalis powder, and the carrier is one of poplar bark powder, willow bark powder, pear bark powder, elm bark powder, locu...

Embodiment 2

[0094] The production method comprises the steps of:

[0095] Paecilomyces cicadae was extracted by water extraction and alcohol precipitation.

[0096] The seeds are then cultivated by the liquid shake flask method, 0.01-5 parts by weight of Paecilomyces cicadae is mixed with the culture medium, the culture medium is 300-680 parts by weight of a mixture of eggs and silkworm chrysalis powder, 10-40 parts by weight of willow bark, 5-10 parts by weight of tea leaves, 1000 parts by weight of water. Wherein, the mixture of eggs and silkworm chrysalis powder is a mixture of raw eggs and silkworm chrysalis powder, or a mixture of cooked egg powder and silkworm chrysalis powder, and the weight ratio of eggs and silkworm chrysalis powder is 1-9:1 or 1:1-9. The culture temperature is 0-30°C, and the culture is carried out under the condition of ventilation for 6-12 days. Paecilomyces cicadae seeds are obtained.

[0097] Primary seed tank fermentation

[0098] In a 50-liter fully au...

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Abstract

The invention relates to a method for producing a preparation with biological bacteria, which is used for preventing plant nematodes and the application of the preparation, in particular to a paecilomyces cicadae biological bacteria preparation, a method for producing the same and the application of the same to plant nematode prevention. The paecilomyces cicadae biological bacteria preparation can be the compound of paecilomyces cicadae, culture medium of the paecilomyces cicadae, auxiliary materials and a carrier and is produced by culturing paecilomyces cicadae, fermenting the paecilomyces cicadae with liquid, propagating the paecilomyces cicadae in great qualities, directly processing the paecilomyces cicadae and absorbing the paecilomyces cicadae with the auxiliary materials and the carrier. The paecilomyces cicadae biological bacteria preparation has obvious effect on plant nematode prevention, stable performance and wide application range, reaches the standard of the quality guarantee period of biological pesticide, meets the production requirements of organic food and green food, is environmental friendly, beneficial to human health and prone to large-scale industrial production and serves as broad-spectrum biological bacteria preparation for preventing plant nematodes.

Description

technical field [0001] The invention relates to a production method and application of a preparation for preventing and controlling plant nematodes prepared by using biological bacteria, in particular to a biological preparation of Paecilomyces cicadae and its preparation method and its application in preventing and controlling plant nematodes. Background technique [0002] Root-knot nematode diseases are common in the world, among which the four species of root-knot nematode southern, northern root-knot nematode, javan root-knot nematode and peanut root-knot nematode are the most common and cause the greatest damage to crops. Root-knot nematode usually does not have obvious host specialization, and it can infect about 114 families and more than 3,000 species of crops. It is estimated that the annual losses caused by plant nematodes in the world are more than 100 billion US dollars. Especially in recent years, with the expansion of the planting area of ​​agricultural protect...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P5/00A01P7/04C12N1/14C12R1/79A01N63/30
CPCC12R1/79A01N63/04C12N1/14A01N63/30C12R2001/79C12N1/145
Inventor 刘伟平王建玲
Owner 刘伟平
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