Cordyceps mycelia and preparation method thereof

A technology of Cordyceps mycelium and Cordyceps mycelium, applied in botany equipment and methods, food preparation, gardening, etc., to achieve the effect of rich content, making up for the shortage of limiting amino acids, and high nutritional value

Active Publication Date: 2013-07-24
TIANJIN TIANSHI BIOLOGICAL DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no corresponding reports on Cordyceps mycelium products rich in calcium, magnesium, iron and zinc and their preparation methods at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Step 1: Strain inoculation and constant temperature culture:

[0035] Preparation medium: its formula composition and weight ratio are: maltose 2%; egg protein powder 5%; KH 2 PO 4 0.3%; magnesium sulfate 0.2%; water balance. The prepared medium was divided into conical flasks, sealed and sterilized at 120°C for 20 minutes, the medium was taken out, naturally cooled to 25°C, and Paecilomyces spp. was inserted under aseptic conditions. Culture at 27°C for 8 days.

[0036] Step 2: Seed tank cultivation:

[0037] Preparation medium: its formula composition and weight ratio are: potato flour 20%; glucose 3%; whey protein concentrate 4%; KH 2 PO 4 0.2%; magnesium sulfate 0.1%; water balance. The prepared culture medium was sterilized under high temperature and high pressure, and the sterilization conditions were: sterilization pressure 0.1 MPa; sterilization temperature 121° C.; sterilization time 30 minutes. After sterilization, insert the strain cultivated in Step 1...

Embodiment 2

[0046] Step 1: Strain inoculation and constant temperature culture:

[0047] Preparation medium: its formula composition and weight ratio are: maltose 1.5%; egg protein powder 4%; KH 2 PO 4 0.2%; magnesium sulfate 0.5%; water balance. The prepared medium was divided into conical flasks, sealed and sterilized at 128°C for 25 minutes, the medium was taken out, naturally cooled to 27°C, and Paecilomyces spp. was inserted under aseptic conditions. Incubate at 30°C for 6 days.

[0048] Step 2: Seed tank cultivation:

[0049] Preparation medium: its formula composition and weight ratio are: potato flour 25%; glucose 2%; whey protein concentrate 3%; KH 2 PO 4 0.4%; magnesium sulfate 0.6%; water balance. The prepared culture medium was sterilized under high temperature and high pressure, and the sterilization conditions were: sterilization pressure 0.1 MPa; sterilization temperature 121° C.; sterilization time 30 minutes. After sterilization, insert the strain cultivated in Ste...

Embodiment 3

[0058] Step 1: Strain inoculation and constant temperature culture:

[0059] Preparation medium: its formula composition and weight ratio are: maltose 3%; egg protein powder 7%; KH 2 PO 4 0.4%; magnesium sulfate 1%; water balance. Divide the prepared medium into conical flasks, seal and sterilize at 120°C for 18 minutes, take out the medium, cool it down to 21°C naturally, insert Paecilomyces spp. under aseptic conditions, Culture at 19°C for 10 days.

[0060] Step 2: Seed tank cultivation:

[0061] Preparation medium: its formula composition and weight ratio are: potato flour 15%; glucose 4%; whey protein concentrate 2%; KH 2 PO 4 0.5%; magnesium sulfate 0.9%; water balance. The prepared culture medium was sterilized under high temperature and high pressure, and the sterilization conditions were: sterilization pressure 0.1 MPa; sterilization temperature 121° C.; sterilization time 30 minutes. After sterilization, insert the bacteria cultured in step 1, the inoculum siz...

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Abstract

The invention discloses cordyceps mycelia and a preparation method thereof. The preparation method of the cordyceps mycelia comprises the following steps of inoculation and constant-temperature cultivation of paecilomyces hepialid, seeding tank cultivation, fermentation tank cultivation, ultralow-temperature vacuum concentration, freeze drying and crushing. In comparison with the cordyceps mycelia produced in the prior art, the cordyceps mycelia produced by the method has the advantages that the calcium content is increased by 90-120 times, the magnesium content is increased by 1-2 times, the contents of ferrum, zinc, cordycepic acid and cordycepin are increased by 20-50%, and the superoxide dismutase (SOD) activity is increased by 20-30%. Therefore, the health efficacy of the product is remarkably improved.

Description

technical field [0001] The invention relates to cordyceps mycelium and a preparation method thereof, belonging to the field of food processing. Background technique [0002] Cordyceps sinensis is a traditional and valuable strong and nourishing medicinal material in my country. Due to the strict parasitic nature and special growth environment of natural cordyceps, coupled with the serious damage to the ecological environment in recent years, the resources of natural cordyceps have decreased sharply and the price has skyrocketed. With the continuous improvement of people's living standards, the demand for Cordyceps is increasing day by day. Therefore, it is imperative to replace natural Cordyceps with artificially cultivated Cordyceps mycelium. Modern scientific research shows that Cordyceps mycelium is rich in functional ingredients such as cordycepic acid, cordycepin, SOD enzyme, and Cordyceps polysaccharide, and its content is basically the same as that of natural Cordycep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/28A23L1/29A01G1/04A23L33/00
Inventor 郁正刚黄永亮
Owner TIANJIN TIANSHI BIOLOGICAL DEV
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