Active additive for promoting agricultural waste material compost decomposition and its application

An active additive and agricultural waste technology, which is applied in the field of enzymatic liquid treatment to promote the composting and decomposing of agricultural waste, and can solve the problems of inability to degrade obviously

Inactive Publication Date: 2006-01-25
HUNAN UNIV
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Problems solved by technology

In the primary fermentation stage of compost, since it contains a large amount of easily degradable organic matter, th

Method used

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  • Active additive for promoting agricultural waste material compost decomposition and its application
  • Active additive for promoting agricultural waste material compost decomposition and its application

Examples

Experimental program
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Embodiment Construction

[0027] 1. Seed culture: Bacillus subtilis (Bacillus subtilis), Aspergillus niger (Aspergillus niger), Trichoderma viride (Trichoderma viride), and Penicillium simplicissimum (Penicillium simplicissimum) were respectively inoculated from the slant medium to beef extract peptone medium, check 200ml medium in a 1L Erlenmeyer flask, cultured at 37°C, 200rpm, for 30h.

[0028] 2. Fermentation culture: Put 4L of fermentation medium in a 5L automatic fermenter, mix the seed culture solution at a ratio of 18%, 35%, 22% and 25%, and then inoculate the mixed seed solution at 8% into the fermenter , cultured at 37°C, 500rpm, 1.2vvm for 4d.

[0029] 3. The obtained fermented liquid is filtered with four layers of gauze to remove bacteria and solid matrix, which is the active additive. After determination, the active additive pectinase activity is 78.4U·ml -1 , xylanase activity is 65.9U·ml -1 , the filter paper cellulase activity is 42.3U·ml -1 , the activity of polyphenol oxidase was...

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Abstract

The invention relates to an enzyme liquid treatment for agricultural waste composts, and provides a fast-rotting reactive additive which is prepared by mixing bacillus, aspergillus niger, trichoderma and blue mould proportionally for fermentation, producing composite enzyme liquid, filtering the liquid state fermentation liquid. The liquid can be applied directly by 0.5-1 wt% of the compost raw material at the beginning of the composts preparation. The prepared reactive additive contains a large quantity of pectin enzyme and ligno-cellulose. The addictive can facilitate the degradation of pectin, hemicellulose, cellulose in one fermentation stage, destruct vegetable cell wall tissue, thus quickening the compost rotting course.

Description

technical field [0001] The invention relates to solid waste treatment, in particular to an enzyme solution treatment for promoting the composting and maturity of agricultural waste. Background technique: [0002] my country is a large agricultural country, and a large amount of agricultural waste produced every year not only affects the environment, but also wastes resources. Aerobic composting is an important means of resourceful and harmless treatment of agricultural waste. Since agricultural waste, especially crop straw, is rich in refractory lignocellulose, it has problems such as long composting treatment cycle and low compost quality. Inoculation of microbial cells is currently a commonly used method to promote the composting process. In the invention patent of ZL 03118137.6 "Twice Inoculation of Microbial Composite Bacteria Composting Method", it is proposed to add 1# composite bacteria in the high temperature stage and the cooling stage of the secondary fermentation ...

Claims

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Application Information

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IPC IPC(8): C05F17/00C05F11/08C12N1/00
CPCY02W30/40
Inventor 郁红艳曾光明黄国和黄红丽
Owner HUNAN UNIV
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