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Paecilomyces lilacinus, culture method of paecilomyces lilacinus and use of paecilomyces lilacinus in prevention and control of diseases and pests of crops

A technology of Paecilomyces lilacinus and Paecilomyces lilacinus, applied to whiteflies and soybean cyst nematodes, in the field of prevention and control of pest aphids in protected areas, and can solve problems such as highly toxic pesticides

Inactive Publication Date: 2014-12-17
谢明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and control of soybean cyst nematodes mainly rely on chemical pesticides, such as dibromochloropropane, dibromoethane, carbofuran, etc. These chemical pesticides for nematode control are mostly highly toxic pesticides

Method used

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  • Paecilomyces lilacinus, culture method of paecilomyces lilacinus and use of paecilomyces lilacinus in prevention and control of diseases and pests of crops
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  • Paecilomyces lilacinus, culture method of paecilomyces lilacinus and use of paecilomyces lilacinus in prevention and control of diseases and pests of crops

Examples

Experimental program
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Effect test

Embodiment 1

[0057] Embodiment 1: the mass culture technology of fungal strain spore

[0058] Cultivate the strain of this strain in a shaker flask at 25°C for 40h to 50h, use it as a seed, connect it to a solid culture composed of corn flour and ceramic balls at a volume of 8%, and cultivate it in a sterile bag at 25°C for 7 days, open the bag and culture for 2 days. After the cultivation, dry in a drying room at 35° C. for 2 days, pulverize the dried culture, and sieve (250 mesh, 320 mesh, 400 mesh) to obtain pure sporopollen (containing 95% spores).

Embodiment 2

[0059] Example 2: Prevention and treatment of protected vegetable greenhouse whitefly and aphids

[0060] The resulting pure sporopollen will be produced according to: pure sporopollen (containing spore 95%) 10%, glucose (powder) 2%, sodium lignosulfonate 1%, starch 3%, dispersant BD553%, wetting agent BENOL790A3 %, diatomaceous earth 78%, evenly mixed. The preparation can be applied in the field. The preparation is diluted 1500 times with water, sprayed to prevent whitefly in greenhouses, and when the indoor temperature is 23-27° C. and the relative humidity is above 85%, the parasitic rate to pests reaches 90% within 6 days.

Embodiment 3

[0061] Embodiment 3: control soybean field cyst nematode disease

[0062] Indoor fermentation adopts liquid culture medium (industrial glucose 25g, yeast extract powder 20g, bean cake powder 35g, NaNO 3 3g,K 2 HPO 4 1.5g, MgSO 4 1g, FeSO 4 0.02g of distilled water (1000ml) in a 5L Erlenmeyer flask, shake culture at 180rmp / min, 25°C for 3-5d. Large-scale industrial production can be scaled up. Carboxymethylcellulose (CMC) is added to the fungal fermentation broth by 0.01% volume, and the liquid preparation is diluted 100 times, and then the soybean field soil is watered. After 90 days, the control effect on soybean cyst nematodes reaches 80%.

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Abstract

The invention discloses a paecilomyces lilacinus strain and its culture method and use. The paecilomyces lilacinus strain is paecilomyces lilacinus CPLI0708, is preserved in the China general microbiological culture collection center (CGMCC) and has an accession number of CGMCC No.8450. The culture method of the paecilomyces lilacinus strain comprises the following steps of A, bacterial strain activation: carrying out bacterial strain streak cultivation activation on a PDA inclined plane, B, bacterial strain liquid fermentation culture: transferring the activated bacterial strain into a triangular flask with a liquid medium and carrying out shaking culture, and C, bacterial strain solid fermentation culture: mixing a carrier, a nutrition matrix and water according to a volume ratio to form a uniform mixture which is a solid fermentation medium, putting the solid fermentation medium into a fresh-keeping bag, inoculating the solid fermentation medium with a liquid fermentation seed, carrying out stirring to obtain a uniform mixture and carrying out culture. The bacterial strain conidiospore has strong effects of killing sucking-type insects such as greenhouse aphids and whitefly and the fermentation broth has strong effects of killing larvas of soybean cyst nematode and can be used for pollution-free vegetable production.

Description

technical field [0001] The invention relates to a strain of Paecilomyces lilacinus and its cultivation method, and its application in preventing and controlling pests aphids, whiteflies and soybean cyst nematodes in protected areas. Background technique [0002] There are more than 50 million mu of planting area of ​​vegetables in protected areas in my country. Vegetables in protected areas are the main source of vegetables for northern residents in winter and spring, and have important national economic significance. The main pests in winter and spring vegetable protection areas are whitefly (whitefly and whitefly, etc.), aphids (melon aphid, etc.), and the perennial damage yield loss is more than 30%. At present, the main method of controlling pests in protected areas is chemical control, such as spraying chemical pesticides such as organophosphorus, pyrethroids, and imidacloprid. The extensive use of these pesticides has caused serious pesticide residues in agricultural p...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04A01P5/00C12R1/79
Inventor 谢明张艳军张召荣
Owner 谢明
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