Technology for extracting cordyceps polysaccharide from Paecilomyces hepiali fermentation broth

A technology of Paecilomyces spp. and Cordyceps polysaccharides, applied in the biological field, can solve the problems of low purity of finished products of Cordyceps polysaccharides, low extraction rate of Cordyceps polysaccharides, unfavorable mass production, etc., achieve low cost, less loss of polysaccharides, and simplify the production process Effect

Active Publication Date: 2017-02-22
山东仙普爱瑞科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing technology has the following defects: (1) the purity of the finished product of Cordyceps polysaccharide is relatively low; (2) the extraction rate of Cordyceps pol

Method used

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  • Technology for extracting cordyceps polysaccharide from Paecilomyces hepiali fermentation broth
  • Technology for extracting cordyceps polysaccharide from Paecilomyces hepiali fermentation broth
  • Technology for extracting cordyceps polysaccharide from Paecilomyces hepiali fermentation broth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 A process for extracting Cordyceps polysaccharide from the fermentation broth of Paecilomyces hematalis

[0034] Extracting the Cordyceps polysaccharide from the Paecilomyces batata fermented liquid comprises the following steps:

[0035] Step 1 Beating

[0036] The formula for the fermentation of Paecilomyces hematalis is 20% potato, 2% soybean flour, 1% sucrose, 0.3% KH 2 PO 4 , 0.15%MgSO 4 ·7H 2 O, 0.001% vitamin B1, and water make up the balance; the above-mentioned percentages are mass percentages; after the fermentation is completed, a fermented liquid is obtained.

[0037] Adjust the pressure of the fermenter to 0.03MPa, adjust the pressure of the beater to zero, and use the pressure difference to press 1000L of the fermented Paecilomyces hematodes fermentation liquid into the beater; add water equal to the volume of the fermentation liquid, turn on the beater for beating, and beat to a homogenous state;

[0038] The rotation speed during beating ...

Embodiment 2

[0058] Example 2 A process for extracting Cordyceps polysaccharide from the fermentation broth of Paecilomyces hematalis

[0059] Extracting the Cordyceps polysaccharide from the Paecilomyces batata fermented liquid comprises the following steps:

[0060] Step 1 Beating

[0061] The formula for the fermentation of Paecilomyces hematalis is 20% potato, 2% soybean flour, 1% sucrose, 0.3% KH 2 PO 4 , 0.15%MgSO 4 ·7H 2O, 0.001% vitamin B1, and water make up the balance; the above-mentioned percentages are mass percentages; after the fermentation is completed, a fermented liquid is obtained.

[0062] Adjust the pressure of the fermenter to 0.03MPa, adjust the pressure of the beater to zero, and use the pressure difference to press 1000L of the fermented Paecilomyces hematodes fermentation liquid into the beater; add water equal to the volume of the fermentation liquid, turn on the beater for beating, and beat to a homogenous state;

[0063] The rotating speed during beating i...

Embodiment 3

[0084] Example 3 A process for extracting Cordyceps polysaccharide from the fermentation broth of Paecilomyces hematalis

[0085] Extracting the Cordyceps polysaccharide from the Paecilomyces batata fermented liquid comprises the following steps:

[0086] Step 1 Beating

[0087] The formula for the fermentation of Paecilomyces hematalis is 20% potato, 2% soybean flour, 1% sucrose, 0.3% KH 2 PO 4 , 0.15%MgSO 4 ·7H 2 O, 0.001% vitamin B1, and water make up the balance; the above-mentioned percentages are mass percentages; after the fermentation is completed, a fermented liquid is obtained.

[0088] Adjust the pressure of the fermenter to 0.03MPa, adjust the pressure of the beater to zero, and use the pressure difference to press 1000L of the fermented Paecilomyces hematodes fermentation liquid into the beater; add water equal to the volume of the fermentation liquid, turn on the beater for beating, and beat to a homogenous state;

[0089] The rotating speed during beating ...

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Abstract

The invention discloses a technology for extracting cordyceps polysaccharide from Paecilomyces hepiali fermentation broth. The technology comprises steps of pulping, water bath extraction, centrifugation, membrane separation and vacuum drying. In the water bath extraction step, the material-water ratio of a thallus breaking liquid to distilled water is (1:1)-(3:1) in volume, the water bath temperature is 85-95 DEG C, and the extraction time lasts for 4-8 h. In the pulping step, the rotating speed of pumping is 16,000-22,000 r/min, and pulping is performed for 1-2 min in every other 1-2 min and performed 2-12 times. The mass percentage of the cordyceps polysaccharide is 90% or higher.

Description

technical field [0001] The invention relates to a process for extracting Cordyceps polysaccharides, in particular to a process for extracting Cordyceps polysaccharides from Paecilomyces hematoptera fermentation liquid, and belongs to the field of biotechnology. Background technique [0002] The active ingredient of Cordyceps sinensis is mainly Cordyceps polysaccharide, which is a polysaccharide composed of mannose, cordycepin, adenosine, galactose, arabinose, xylose, glucose, and fucose. [0003] A large number of research data at home and abroad show that Cordyceps polysaccharides have physiological activities such as anti-tumor, anti-aging, anti-bacterial, and lowering blood sugar. Viruses and microorganisms etc. Therefore, Cordyceps polysaccharide has broad development prospects as health food and pharmaceutical products. [0004] At present, the purification of Cordyceps polysaccharides mostly adopts the traditional method: water extraction and alcohol precipitation. ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 郭海岩刘刚王茂超王兴业韩国英刘东东郝利利郭芹王红军
Owner 山东仙普爱瑞科技股份有限公司
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