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Application of Cordyceps fungal cell metabolites in preparation of skin-care products and skin-care product

A technology of metabolites, fungal cells, applied in the field of action in acne

Active Publication Date: 2018-01-30
徐跃
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] To sum up, in the existing published patents and published papers, there is no discussion about the efficacy and application of Cordyceps fungus metabolites, especially high molecular compound glycopeptides, in eliminating acne

Method used

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  • Application of Cordyceps fungal cell metabolites in preparation of skin-care products and skin-care product
  • Application of Cordyceps fungal cell metabolites in preparation of skin-care products and skin-care product
  • Application of Cordyceps fungal cell metabolites in preparation of skin-care products and skin-care product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Example 1 Purification and domestication of a single strain of Cordyceps fungus

[0089] Fungi isolated from fungal complexes such as wild Cordyceps sinensis are sometimes symbiotic, containing many symbiotic bacteria or other organisms, but appear as a single species on agar medium. There is no problem with solid fermentation, but with liquid submerged fermentation, occasional unexplained fermentation contamination often occurs. Therefore, all isolated fungal strains need to be isolated purely. Take Cordyceps sinensis, or Hirsutella hepiali Chen et Shen, or Hirsutella hepiali Chen et Shen as an example.

[0090] Purebred separation steps:

[0091] 1) Cordyceps strains isolated from wild species (symbiotic species)

[0092] 2) Add chloramphenicol and gentamycin to the peptone glucose liquid medium; after inoculation, cultivate it on a shaker at 25°C for 3-4 weeks

[0093] 3) Inoculate Bangladesh red chloramphenicol agar medium; cultivate at 25°C for 3 weeks, and the...

Embodiment 2

[0115] Example 2 Production of Cordyceps fungus thalline cells by submerged liquid culture

[0116] Fermentation method:

[0117] Step 1: In order to produce cells of the Cordyceps fungus, the isolated CSX series or HHX series strains (CS-X100 or HHX-100 are used in this embodiment) are first cultured on solid medium. Cultivate on potato dextrose agar medium (PDA) slant or plate agar medium (see Table a) for 3 days at 25°C.

[0118] Step 2: 1 liter of liquid culture medium (table b) is packed into 5 liters of Erlenmeyer flasks, after sterilizing at 121 ℃ for 20 minutes, inoculate 3-6 inclined plates of Cordyceps sinensis strains, then grow under the following conditions: pH value 5.5 ( Adjustment before inoculation), 25°C, shake flask rotation speed 150rpm, incubation time 36 hours.

[0119] Step 3: Cultivate secondary seeds Use a stirred bioreactor of 75 liters (or larger), with a liquid volume of 50 liters or more in the fermentation medium (Table c), sterilize at 121°C fo...

Embodiment 3

[0128] Example 3 Preparation of coarse-grade Cordyceps fungus acne-removing concentrated liquid preparation

[0129] Harvest 400 kilograms of fermented yeast liquid produced by the above-mentioned fermentation process, that is, the extracellular metabolite of the Cordyceps fungus, containing 6% of solids, that is, 24 kilograms; it is concentrated to 16% solids in a vacuum concentrator. Add 0.48 kg of activated carbon to about 150 kg of liquid, the ratio is 2% of the solid, stir at 80°C for 30 minutes, then filter or centrifuge to remove the activated carbon, and continue to concentrate the filtrate to 65% of the solid with a vacuum concentrator °Bx, get 25 kg. 25 liters of 99% alcohol were mixed with 25 kg of concentrate, stirred for 4 hours, and then the alcoholic phase was separated from the precipitated phase. Add a small amount of water to the precipitated phase and put it into a vacuum concentrator to thoroughly remove residual alcohol and concentrate to 65 ° Bx of solid...

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Abstract

The invention provides application of Cordyceps fungal cell metabolites in the preparation of skin-care products and a skin-care product; Cordyceps fungi herein at least include any one of Cordyceps sinensis, Hirsutella sinensis, Paecilomyces hepiali, Hirsutella hepiali, Cordyceps militaris, Cordyceps algae, Cordyceps ophioglossoides, Cordyceps hawkesii, Cordyceps martialis, Ophiocordyceps sobolifera, and Cordyceps fruit flies. The Cordyceps fungal cell metabolites herein are not typical antibiotics, but provide germ-killing action; the Cordyceps fungal cell metabolites are not typical anti-inflammatory agents, but have an inflammation diminishing function and are natural and free of side effects.

Description

technical field [0001] The present invention relates to the use of a cell metabolite of the fungus of the genus Cordyceps in the preparation of skin care products and the skin care products, especially the effect on acne removal. Background technique [0002] Acne is one of the most common diseases in dermatology, also known as comedo, pimple or acne, folliculitis. The occurrence of acne is generally related to excessive androgen secretion, increased sebum secretion, hyperkeratosis of hair follicle ducts, infection of Propionibacterium acnes, environmental factors, genetic factors and destruction of sebum membrane. Acne is a typical "teenage Werther's troubles", 4 / 5 young people aged 12-25 will have acne problems. [0003] Drug treatment is basically divided into four categories, namely contraceptives, androgen blockers, antibiotics, retinoic acid / retinoic acid. Retinoic acid / retinoic acid drugs are the first-line drugs for the treatment of inflammatory acne. The mechanism...

Claims

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Application Information

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IPC IPC(8): A61K8/9728A61K8/73A61Q19/00A61Q17/00A61K36/068A61P17/10A61P31/04A61P31/02A61P29/00
Inventor 徐跃
Owner 徐跃
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