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Preparation method of peptone specially used for producing paecilomyces hepiali powder through submerged fermentation

A bat moth Paecilomyces spp., deep fermentation technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions to achieve the effects of complete hydrolysis, abundant sources and low production costs

Inactive Publication Date: 2015-11-11
南通凯恒生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no specific peptone for the fermentation and development of Paecilomyces spp. powder in the market for peptone products, and there is no relevant patent report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] The bones, viscera, and lean meat of pigs and cattle are used as raw materials. The weight proportion ranges are 22% of bovine bone, 22% of pork bone, 8% of bovine viscera, 8% of porcine viscera, 15% of refined pork and 25% of refined beef. Put the prepared raw materials in a basin, rinse with 40°C water, crush each raw material with a corresponding machine, the bone area does not exceed 2cmX2cm, grind the internal organs and lean meat twice with a meat grinder, then mix all the raw materials and soak in water , the ratio of adding water is 1.0 times of the raw material. Put the prepared raw materials into the high-pressure steam furnace, raise the pressure to 0.1Mpa, keep the pressure balance, and keep the temperature at 150°C, cook for 4 hours, and let the dirty oil layer into layers to facilitate the soup. Cool the bone broth and control it at 45°C, adjust the pH to 7.0, add bovine trypsin, and carry out enzymatic hydrolysis for 1 hour. When the pH drops to 6.0, rai...

example 2

[0020] The bones, viscera, and lean meat of pigs and cattle are used as raw materials. The range of weight proportions is 28% of bovine bone, 28% of pork bone, 12% of bovine viscera, 12% of porcine viscera, 5% of refined pork and 15% of refined beef. Put the prepared raw materials in a basin, rinse them with 50°C water, crush each raw material with a corresponding machine, the bone area does not exceed 2cmX2cm, grind the internal organs and lean meat with a meat grinder for 3 times, then mix all the raw materials and soak them in water , the ratio of adding water is 1.2 times of the raw material. Put the prepared raw materials into the high-pressure steam furnace, raise the pressure to 0.2Mpa, keep the pressure balance, and keep the temperature at 180°C. Cook for 7 hours to allow the dirty oil to separate into layers for easy soup release. Cool the bone broth and control it at 55°C, adjust the pH to 8.0, add bovine trypsin, and carry out enzymatic hydrolysis for 3 hours. When...

example 3

[0023] The bones, viscera, and lean meat of pigs and cattle are used as raw materials. The weight proportion ranges are 25% of bovine bone, 23% of pork bone, 11% of bovine viscera, 9% of porcine viscera, 7% of refined pork and 25% of refined beef. Put the prepared raw materials in a basin, rinse with 45°C water once, crush each raw material with a corresponding machine, the bone area does not exceed 2cmX2cm, grind the internal organs and lean meat twice with a meat grinder, then mix all the raw materials and soak in water , the ratio of adding water is 1 times of the raw material. Put the prepared raw materials into the high-pressure steam furnace, raise the pressure to 0.2Mpa, keep the pressure balance, and keep the temperature at 170°C, cook for 5 hours, and let the dirty oil layer into layers to facilitate the soup.

[0024]Cool the bone broth and control it at 50°C, adjust the pH to 7.0, add bovine trypsin, and carry out enzymatic hydrolysis for 2 hours. When the pH drops...

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PUM

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Abstract

The invention belongs to the technical field of fermentation of staple medical fungi. Paecilomyces hepiali is an important substitute product of wild cordyceps sinensis, about 3000 tons of liquid fermented powder of paecilomyces hepiali is needed in China each year, the production value of the product taking the liquid fermented powder of paecilomyces hepiali as the main raw material breaks through 5 billion yuan, and animal peptone is the main nitrogen source for producing the fermented powder of paecilomyces hepiali. The invention specifically relates to a preparation method of peptone specially used for producing paecilomyces hepiali powder through submerged fermentation. The preparation method comprises the following steps: mixing pork material and beef material, carrying out high-pressure steaming, carrying out enzymolysis, carrying out concentrating, and carrying out spray drying, thereby obtaining the peptone product. Under the condition of a 10-ton fermentation tank and with the same cost, by using the peptone provided by the invention for replacing the common peptone to carry out fermentation production of paecilomyces hepiali powder, the yield and the quality of the paecilomyces hepiali powder are obviously improved.

Description

technical field [0001] The invention relates to the technical field of fermentation of bulk medicinal fungi varieties, in particular to a method for preparing a peptone product specially used for submerged fermentation of Paecilomyces spp. technical background [0002] Cordyceps sinensis (Cordyceps sinensis) is a traditional Chinese medicinal material in my country. It has the functions of inhibiting tumor growth and enhancing the body's immunity. Because the growth environment of Cordyceps sinensis is extremely harsh, and the excavation is before the spores of Cordyceps fungus mature and fall off, it brings a devastating blow to the wild resources of Cordyceps sinensis. In addition, the artificial cultivation technology of Cordyceps sinensis has not yet been broken through. These factors lead to limited resources and high prices of wild Cordyceps sinensis. How to solve this problem? [0003] The researchers used the Cordyceps fungus-Paecilomyceshepialichen&dai strain isol...

Claims

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Application Information

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IPC IPC(8): C12P21/06C12N1/14C12R1/79
Inventor 李新章陈龙戴任祥顾锦华
Owner 南通凯恒生物科技发展有限公司
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