Fermented cordyceps sinensis mycelium powder and preparation method thereof

A technology of mycelium powder and fermentation of Cordyceps, applied in biochemical equipment and methods, methods based on microorganisms, fungi, etc., can solve the problems of lack of preparation methods, achieve high nutritional value, rich content, and promote growth

Inactive Publication Date: 2017-09-01
TIANJIN TIANSHI BIOLOGICAL DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is almost no Cordyceps mycelium product rich in calci...

Method used

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  • Fermented cordyceps sinensis mycelium powder and preparation method thereof
  • Fermented cordyceps sinensis mycelium powder and preparation method thereof
  • Fermented cordyceps sinensis mycelium powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Step 1: Strain inoculation and constant temperature culture:

[0086] Preparation medium: its formula composition and weight ratio are: 2% maltose, 5% egg protein powder, KH 2 PO 4 0.3%, magnesium sulfate 0.2%, water balance. The prepared medium was divided into conical flasks, sealed and sterilized at 120°C for 20 minutes, the medium was taken out, naturally cooled to 25°C, and Paecilomyces spp. was inserted under aseptic conditions. Culture at 27°C for 8 days.

[0087] Step 2: Seed tank cultivation:

[0088] Preparation medium: its formula composition and weight ratio are: potato flour 20%, glucose 3%, whey protein concentrate 4%, KH 2 PO 4 0.2%, magnesium sulfate 0.1%, water balance. The prepared culture medium was sterilized under high temperature and high pressure, and the sterilization conditions were: sterilization pressure 0.1 MPa; sterilization temperature 121° C.; sterilization time 30 minutes. After sterilization, insert the cultured bacteria in step ...

Embodiment 2

[0098] Step 1: Strain inoculation and constant temperature culture:

[0099] Preparation medium: its formula composition and weight ratio are: maltose 1.5%, egg protein powder 4%, KH 2 PO 4 0.2%, magnesium sulfate 0.5%, water balance. The prepared medium was divided into conical flasks, sealed and sterilized at 128°C for 25 minutes, the medium was taken out, naturally cooled to 27°C, and Paecilomyces spp. was inserted under aseptic conditions. Incubate at 30°C for 6 days.

[0100] Step 2: Seed tank cultivation:

[0101] Preparation medium: its formula composition and weight ratio are: potato flour 25%, glucose 2%, whey protein concentrate 3%, KH 2 PO 4 0.4%, magnesium sulfate 0.6%, water balance. The prepared culture medium was sterilized under high temperature and high pressure, and the sterilization conditions were: sterilization pressure 0.1 MPa; sterilization temperature 121° C.; sterilization time 30 minutes. After sterilization, insert the cultured bacteria in ste...

Embodiment 3

[0111] Step 1: Strain inoculation and constant temperature culture:

[0112] Preparation medium: its formula composition and weight ratio are: maltose 3%, egg protein powder 7%, KH 2 PO 4 0.4%, magnesium sulfate 1%, water balance. Divide the prepared medium into conical flasks, seal and sterilize at 120°C for 18 minutes, take out the medium, cool it down to 21°C naturally, insert Paecilomyces spp. under aseptic conditions, Culture at 19°C for 10 days.

[0113] Step 2: Seed tank cultivation:

[0114] Preparation medium: its formula composition and weight ratio are: potato flour 15%, glucose 4%, whey protein concentrate 2%, KH 2 PO 4 0.5%, magnesium sulfate 0.9%, water balance. The prepared culture medium was sterilized under high temperature and high pressure, and the sterilization conditions were: sterilization pressure 0.1 MPa; sterilization temperature 121° C.; sterilization time 30 minutes. After sterilization, insert the cultured bacteria in step 1, the inoculum si...

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Abstract

The invention discloses fermented cordyceps sinensis mycelium powder and a preparation method thereof. The preparation method comprises the following steps of performing inoculation with paecilomyces hepiali strain, performing constant-temperature culture, performing culture in a seeding tank, performing culture with a fermenter, performing ultralow temperature vacuum concentration, performing freeze drying and performing crushing. Compared with cordyceps sinensis mycelium powder produced in the patent of which the application number is 201310124056.3, the cordyceps sinensis mycelium powder made by the method disclosed by the invention has the advantages that the content of calcium in the cordyceps sinensis mycelium powder is increased by 15-20%, the content of magnesium is increased by 5-8%, the content of iron is increased by 5-7%, the content of zinc is increased by 6-9%, the content of cordycepic acid is increased by 5-8%, the content of cordycepin is increased by 6-10%, and the activity of SOD enzyme is improved by 4-7%. The invention further discloses an application of using the cordyceps sinensis mycelium powder as an active component in preparing a preparation for improving the immune function of organisms.

Description

technical field [0001] The invention relates to a fermented cordyceps mycelium powder, its preparation method and its application in the preparation of preparations for enhancing immune function. Background technique [0002] Cordyceps sinensis is a traditional and valuable strong and nourishing medicinal material in my country. Due to the strict parasitic nature and special growth environment of natural cordyceps, coupled with the serious damage to the ecological environment in recent years, the resources of natural cordyceps have decreased sharply and the price has skyrocketed. With the continuous improvement of people's living standards, the demand for Cordyceps is increasing day by day. Therefore, it is imperative to replace natural Cordyceps with artificially cultivated Cordyceps mycelium. Modern scientific research shows that Cordyceps mycelium is rich in functional ingredients such as cordycepic acid, cordycepin, SOD enzyme, and Cordyceps polysaccharide, and its conte...

Claims

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Application Information

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IPC IPC(8): A23L31/00A23L33/00C12N1/14C12R1/645
CPCA23L31/00A23L33/00A23V2002/00C12N1/14A23V2200/324
Inventor 黄永亮郁正刚
Owner TIANJIN TIANSHI BIOLOGICAL DEV
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