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Novel application of neutral extracellular polysaccharides of paecilomyces hepiali

A technology of Paecilomyces spp. and Paecilomyces spp. applied in the field of neutral exopolysaccharides of P. spp. can solve problems such as increasing the production of short-chain fatty acids and lactic acid

Active Publication Date: 2015-12-16
HENAN INST OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Yu et al. used an in vitro fermentation model to study the regulation of polyglycopeptide (PSP) on the intestinal flora and the effect on the production of short-chain fatty acids in the system. The results showed that PSP can significantly increase the ratio of bifidobacteria and lactic acid bacteria in the fermentation system. , reduce the ratio of Clostridium, Staphylococcus, and Enterococcus, but have no significant effect on Escherichia coli, and the determination of short-chain fatty acids shows that PSP can increase the production of short-chain fatty acids such as acetic acid, propionic acid, butyric acid and lactic acid
However, there has been no report on the activity of the exopolysaccharide of Paecilomyces batatus in this respect. Therefore, this study deeply studied the effect of the exopolysaccharide produced by the bacteria on the intestinal microorganisms and the intestinal microenvironment. The research results It can provide a theoretical basis for the development of this polysaccharide as a prebiotic product

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of neutral exopolysaccharide from Paecilomyces basilisk moth:

[0027] (1) From the parent species of Paecilomyceshepiali (Paecilomyceshepiali) HN1 strain preserved in potato slant medium, excavate a piece of mycelium with a size of about 0.5cm×0.5cm, and inoculate it in a capacity of 100mL seed medium In a 250mL Erlenmeyer flask, after static culture at 20°C for 12 hours, shake culture at 120rpm for 72 hours to obtain seed liquid. The composition of the seed medium is: glucose 30.00g / L, yeast extract 6.00g / L, potassium dihydrogen phosphate 1.50g / L, magnesium sulfate 0.50g / L, wort juice 10.00g / L, potato juice 10.00g / L, pH6 .5;

[0028] (2) Get the seed liquid in the step (1) and inoculate it into a 500 mL Erlenmeyer flask with a capacity of 200 mL of fermentation medium, insert the seed liquid by 2% of the volume of the fermentation medium, and cultivate it statically for 12 hours at 20° C. Then, shake culture at 120 rpm for 120 hours, centrifuge the ferme...

Embodiment 2

[0033] Metabolic Changes of Neutral Exopolysaccharides from Paecilomyces spp. in Anaerobic System

[0034] Depend on figure 1 It can be seen that the neutral exopolysaccharide components of Paecilomyces bat mothi can be better decomposed and utilized by intestinal microorganisms in the anaerobic fermentation system of human feces samples. At 12 hours of anaerobic fermentation, part of the polysaccharide components had been hydrolyzed into small molecular sugars, and at 24 hours, most of the polysaccharides had been hydrolyzed. As the fermentation time prolongs, it can be seen that the content of polysaccharides and small molecule sugars after hydrolysis gradually decreases, and at 48 hours after the end of fermentation, almost all the neutral exopolysaccharides of Paecilomyces hematilis are absorbed by the intestinal tract in the fermentation system. Microorganisms are exhausted.

Embodiment 3

[0036] Effects of polysaccharides from Paecilomyces hematodes on intestinal flora

[0037] Studies have shown that some functional polysaccharides can affect the structure of intestinal flora, and then have beneficial effects on the body. To this end, we selected and monitored the proliferation of five genera of microorganisms that are closely related to intestinal health to evaluate the effects of adding different polysaccharide components on the intestinal flora in the in vitro fermentation system. The results are shown in Table 1.

[0038] Table 1 Changes of the bacterial flora of Paecilomyces bat moth in vitro anaerobic fermentation process of neutral exopolysaccharide

[0039]

[0040] * Different lowercase letters in each group indicate significant differences in experimental results (p<0.05).

[0041] It can be seen from Table 4-2 that, compared with the blank control group, adding the prebiotic fructo-oligosaccharide (FOS) significantly increased the two probiotics...

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Abstract

The invention discloses novel application of neutral extracellular polysaccharides of paecilomyces hepialid. The paecilomyces hepiali is an important endophytic fungus in late growth and development phases of natural cordyceps sinensis, and polysaccharide components which are secreted by the paecilomyces hepiali into fermentation liquid in liquid fermentation procedures have diversified biological activities. The novel application has the advantages that treatment effects of the neutral extracellular polysaccharides in the aspects of intestinal flora and micro-environment regulation are deeply developed by the inventor on the basis of mass pharmacodynamic study on the neutral extracellular polysaccharides of the paecilomyces hepiali, and the novel application of the neutral extracellular polysaccharides of the paecilomyces hepiali is increased.

Description

technical field [0001] The present invention relates to a new application of the neutral exopolysaccharide of Paecilomyces hematalis, in particular to the use of the neutral exopolysaccharide of Paecilomyces hematalis in improving intestinal flora and microenvironment. Background technique [0002] Paecilomyceshepiali is an important endophytic fungus in the late stage of differentiation and maturity of natural Cordyceps sinensis. The chemical composition analysis shows that the chemical composition of Paecilomyceshepiali is closer to that of natural Cordyceps sinensis. It contains cordycepin, mannitol, and Cordyceps Polysaccharides, alkaloids and other biologically active substances have various biological functions such as anti-oxidation, anti-tumor, anti-radiation, and regulation of the body's immunity. At present, liquid fermentation using endophytic fungi of artificial Cordyceps has become an important source of artificial Cordyceps products. Among them, we found that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P1/00C12P19/04C12R1/79
Inventor 武忠伟李勇超陈向东康壮丽王明艳何承云
Owner HENAN INST OF SCI & TECH
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