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Trichoderma koningii and bacterial agent containing same and application thereof in prevention of cylindrocarpon destructans

A technology of Trichoderma and microbial inoculants, applied in the application, fungicides, biocides and other directions, can solve the problem that the control effect of ginseng rust rot is not ideal, and achieve the effect of high control effect

Active Publication Date: 2015-04-08
HAINAN JINYUFENG BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the microorganisms currently used to control ginseng rust and rot are not ideal for the control of ginseng rust and rot, and new microbial resources need to be discovered.

Method used

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Experimental program
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Embodiment approach

[0030] Wherein, as a particularly preferred embodiment of the present invention, based on the total weight of the Trichoderma liquid fermentation medium, calculated by weight ratio, the Trichoderma liquid fermentation medium contains 2.0-3.5% starch, 1.0- 2.0% yeast powder, 4.0-8.0% corn steep liquor, 0.1-0.5% CaCO 3 , 0.01-0.02% ZnSO 4 and 0.01-0.03% MgSO 4. Wherein, the culture medium may also contain the rest of water and / or other components. In this preferred mode, on the one hand, Trichoderma can be allowed to grow rapidly during the fermentation process to produce a sufficient number of chlamydospores; application in disease control.

[0031] Among them, as a particularly preferred embodiment of the present invention, the liquid fermentation medium of Bacillus subtilis and Bacillus amyloliquefaciens contains 1-1.5% corn starch, 1-1.5% peanut cake powder, 0.2-0.5% ammonium sulfate, 0.1-0.4% calcium carbonate, 0.02-0.05% magnesium sulfate and 0.01-0.05% sodium chlorid...

Embodiment 1

[0039] This embodiment is used to illustrate the cultivation of Trichoderma of the present invention and the preparation of microbial inoculum.

[0040] The Trichoderma (Trichoderma koningii) whose preservation number is CGMCC No.9726 is inoculated into the activated medium PDA medium (get the potato 20g after peeling and boil for 20 minutes to get the juice, and the juice is made into 2g glucose, 1.5g agar and water) 100ml, pH6.8-7.0, packed into 2 eggplant bottles, each 50ml, sterilized and made on a slant), and cultivated at 28°C for 6 days. The Trichoderma obliquus bacterial classification that will activate is inoculated to the liquid fermentation medium of sterilization (by weight, containing 2.5% starch, 1.5% yeast powder, 5.0% corn steep liquor, 0.4% CaCO 3 , 0.01% ZnSO 4 , 0.03% MgSO 4 ), cultivated at 28° C. for 144 hours, until 90% of mycelia formed chlamydospores, and obtained Trichoderma liquid.

[0041] Centrifuge 5000mL of the above-mentioned Trichoderma liqu...

Embodiment 2

[0043] This embodiment is used to illustrate the preparation of the composite microbial bacterial agent of the present invention.

[0044] The commodity number is the Bacillus subtilis (Bacillus subtilis) of ACCC 03221 inoculated on the slant of LB medium (containing the tryptone of 10g / L, the yeast extract of 5g / L, the NaCl of 10g / L and 15g / L agar), Cultivate at 37°C for 18 hours to obtain activated strains. Inoculate the liquid fermentation medium (by weight, containing 1% cornstarch, 1.5% peanut powder, 0.5% ammonium sulfate, 0.4% calcium carbonate, 0.03% magnesium sulfate, 0.02% chlorine sodium chloride), cultured at 35°C until the concentration of viable bacteria was 5-10×10 9 CFU / mL to obtain the Bacillus subtilis bacterial liquid.

[0045] Inoculate Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) with product number ACCC 10166 on the slant of LB medium (containing 10g / L tryptone, 5g / L yeast extract, 10g / L NaCl and 15 / L agar) , cultivated at 37°C for 16 hours ...

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Abstract

The invention provides trichoderma koningii with a collection number CGMCC No.9726. The invention also provides a compound microorganism bacterial agent; and the active ingredients of the compound microorganism bacterial agent include the trichoderma koningii, bacillus subtillis and bacillus amyloliquefaciens. The invention also provides application of the trichoderma koningii or the microorganism bacterial agent in the prevention of cylindrocarpon destructans. Through the technical scheme, the trichoderma koningii and the compound microorganism bacterial agent can effectively prevent cylindrocarpon destructans.

Description

technical field [0001] The invention relates to the field of microorganism application, in particular to a Trichoderma koningii, microbial inoculants containing the Trichoderma koningii and their application in the prevention and treatment of ginseng rust and rot. Background technique [0002] Ginseng rust and rot is one of the most serious and common diseases in ginseng production, and it is the primary disease of ginseng root diseases. Rust rot occurs in countries and regions where ginseng is grown. Ginseng rust and rot is a typical soil-borne root disease, which occurs from seedling stage to harvest stage. The longer the growth period of ginseng, the heavier the disease and the greater the loss. According to statistics, the current average annual incidence rate in my country is 20%-30%, and it is as high as 70%-80% in some severe plots, with an average loss rate of 20%-30%, which seriously affects the ginseng production, processing quality and commodity value, causing H...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
CPCA01N63/10A01N63/30C12N1/145C12R2001/885A01N2300/00
Inventor 李梅陈书华蒋细良汪浩
Owner HAINAN JINYUFENG BIOLOGICAL ENG
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