54 results about "Trichoderma pseudokoningii" patented technology

The invention discloses a composite bio-formulation for preventing crop diseases, comprising the following raw materials: trichoderma pseudokoningii fermenting liquid, bacillus subtilis fermenting liquid, water-soluble chitosan, sodium glutamate residue liquid and potassium humate, wherein the trichoderma pseudokoningii and the bacillus subtilis are high-effect strains which are obtained after being screened and processed, later the fermenting liquid of the trichoderma pseudokoningii and the bacillus subtilis is obtained through a submerged fermentation technique, and the composite bio-formulation for preventing crop diseases is obtained through combining. The composite bio-formulation for preventing crop diseases in the invention has obvious effects for preventing blight, verticillium, gray mold, phytoph-thora capsici leonian, downy mildew, powdery mildew, gray mold, fulvia fulva and so on, and can be especially used for preventing the blight and the verticillium which have large danger in agriculture. The invention is suitable for preventing the diseases and improving yields of various vegetables, melons, fruits and crops, such as tomatoes, chili peppers, eggplants, cucumbers, watermelons, apples, Chinese chives, cottons and so on.

The invention discloses an unpolluted, green and high-efficiency composite biological soil modifier which comprises the following components: trichoderma pseudokoningii fermentation liquor, bacillus subtilis fermentation liquor, water-soluble chitin, potassium humate and decomposed organic matter. The trichoderma pseudokoningii and the bacillus subtilis are high-efficiency bacterial strains which are obtained through screening and mutating, the trichoderma pseudokoningii fermentation liquor and the bacillus subtilis fermentation liquor are obtained by a liquor submerged fermentation technology, and the unpolluted, green and high-efficiency composite biological soil modifier can be prepared in a combination way. The soil modifier has the following functions that the physicochemical property of the soil can be improved, the hardening of the soil can be broken up, the volume weight of the soil can be reduced, the air permeability of the soil can be improved, the microbiological activity of the soil can be promoted, the growth of the plant root system can be promoted, the disease resistance of the crop can be enhanced, the yield of the crop can be improved, the quality of the crop can be improved, the original ecology of the crop can be recovered and the like, and the soil modifier is the novel soil modifier which is high-efficiency, environment-friendly and convenient to use.

The invention relates to a trichoderma pseudokoningii (SMF2) strain and application thereof, which belong to the technical field of microbiology. The invention discloses the trichoderma pseudokoningii (SMF2) strain preserved in China Center for Type Culture Collection on February 24, 2009 with the preservation number being CCTCCNO: M209031, and the application of the strain to preparation of soil microbial agents of trichoderma preparations. The trichoderma pseudokoningii (SMF2) strain disclosed by the invention can be used for producing plant disease fungus cell wall digestive enzymes (CWDEs) and antibiotics of peptaibols at high yield, the CWDEs and the peptaibols cooperate in situ, and the inhibition effect of the trichoderma pseudokoningii strain to the plant disease fungus is further enhanced.

The invention provides a soil improvement and anti continuous cropping multifunctional biological agent which contains the mixed microorganism of trichoderma pseudokoningii and bacillus subtilis, faveolate humic acid and zinc sulphate; the trichoderma pseudokoningii is a bacterial strain obtained by separating and screening maize straw; the bacterial strain is named after (Trichoderma pseudokoningii) DLDI and is preserved in China general microbiological culture collection (CGMCC), and the number thereof is CGMCC NO.3221; the bacillus subtilis obtained by separating soil is named after (bacillus subtilis) DLD2 and is preserved in China general microbiological culture collection (CGMCC), and the number is CGMCCNO.3222; the biological agent has the effects that (1) biocontrol microorganism is used for blocking harmful flora from infecting plants and killing pathogenic bacteria, induces the plants to generate disease resistance and prevents dead seedlings from rotting roots; (2) the microorganism and the faveolate humic acid are compounded to induce the root system to grow, thus realizing root regeneration and plant regeneration and inhibiting the anti continuous cropping diseases; and (3) the faveolate humic acid and the zinc sulphate are used for breaking the soil hardening of the soil, forming the crumble structure, realizing no deep ploughing, retaining water, alleviating fertilizer damage and increasing the soil productivity.

The preparation method of organic ecological soilless culture medium belongs to the field of agricultural bio technology. It is made up by using 100 portions of straw powder, 50-60 portions of cumulose fermented livestock and fowl dried excrements and 3-5 portions of compound bacterial preparation formed from azotogen and trichoderma preparation through the processes of uniformly mixing and stirring. The preparation of azotogen adopts cellulose Nocardia HD-86 with functions of decomposing cellulose and fixing nitrogen as strain and adds the corn stover, bran and water, and utilizes the aseptic fermentation process to obtain the azotogen, and the preparation of trichoderma preparation uses pseudo-Kan's trichodermin S28 as strain, and uses cob, bran, ammonium sulfate as culture medium through the processes of pulverizing, mixing, moistening, inoculating S28 and fermentation so as to obtain the trichoderma preparation. Said invention is favorable for industrial production of vegetables.

The invention relates to a soil conditioner as well as a preparation method and application of the soil conditioner. The soil conditioner comprises the components based on parts by weight: 10-15 parts of biochemical fulvic acid, 10-20 parts of polyacrylamide, 5-10 parts of burdock oligosaccharide, 5-10 parts of chitosan, 1-5 parts of zinc sulfate, 1-2 parts of boric acid and 50-70 parts of trichoderma pseudokoningii fermentation liquor. The invention also provides the preparation method and the application of the soil conditioner. According to the invention, biochemical fulvic acid and the polyacrylamide are combined, and trace elements zinc and boron, the disease-resistant elicitor burdock oligosaccharide, chitosan and the biological control fungi trichoderma pseudokoningii which are necessary to plants and deficient in the soil are loaded, so that the soil conditioner having the functions of improving the physicochemical property of the soil, inhibiting the soil-borne diseases, promoting the growth of the plants and inducing the resistance of botanical system is prepared, and is environment-friendly.

The invention discloses a composition for preventing plant diseases. The composition comprises, by weight, allicin, sterilizing agents, bacillus subtilis fermentation liquid, trichoderma pseudokoningii fermentation liquid, water-soluble chitin, monosodium glutamate residual liquid, fulvic acid potassium, boric acid, monopotassium phosphate, kasugamycin, methionine, thiamine, momordica grosvenori, polygonatum, astragalus, goldthread, asarum, honeysuckle, rosin, hot pepper and green Chinese onion. The allicin and the sterilizing agents serve as active components and are complexly combined, obvious synergistic interaction can be obtained, sterilizing and disease prevention capacities are improved, sterilizing spectrums are widened, the production of crops is improved, harmful bacteria is killed, diseases are restrained, residues are omitted, drug resistance is not easily generated, prevention and treatment of the plant diseases are improved, disease resistance of plants is improved, roots of the plants are lengthened and increased, stems of the plants are thickened, chlorophyll is increased, seeds of food crops are increased, grains of the food crops are plumped, ears and corners of the food crops are increased, fruits of fruit trees are increased and enlarged, and good quality and high production are obtained.

The invention relates to a production process for bioorganic fertilizer with high bacteria content. The production process comprises the following steps: separately activating a trichoderma viride strain and a trichoderma pseudokoningii strain; separately activating a bacillus amyloliquefaciens strain, a bacillus subtilis strain and a candida utilis strain; carrying out step-by-step enlarged culture and the like. A preparation method provided by the invention has the advantages of low cost, simple process, high operability and convenience in popularization and application; the obtained bioorganic fertilizer is high in content of effective living bacteria; a large amount of corn stalk resources can be really and efficiently converted, soil is remediated, the fertility of the soil is improved, and wastes are changed into valuables; in addition, the problem of environmental pollution caused by straw burning can be relived to a certain degree.

The invention belongs to the technical field of prevention and control of plants diseases and particularly relates to a trichoderma koningiopsis T5-1 bacterial strain and an application thereof in improvement of growth of panax notoginseng and prevention and control of root rot. The bacterial strain is named trichoderma koningiopsis, and is assigned the accession number of CGMCC No. 12779 in China General Microbiological Culture Collection Center. The trichoderma koningiopsis T5-1 bacterial strain has developed mycelia and fluffy and dense aerial mycelia, and is free of generation of pigments. The trichoderma koningiopsis T5-1 bacterial strain can improve growth of panax notoginseng and also can inhibit growth of root rot fungus. The bacterial strain can be hyper-parasitized on the root rot fungus, so that spore production of the root rot fungus is inhibited by means of volatile organic compounds from the bacterial strain in a sealed condition. The bacterial strain lays a good foundation for development and utilization of bio-bacterial fertilizers.

The invention relates to a Trichoderma koningiopsis TZ-11 bacterial strain, an application and a method for preparing a preparation of the Trichoderma koningiopsis TZ-11 bacterial strain. According to the bacterial strain, the wettable powder prepared by conducting solid fermentation can be used for preventing and treating the anthracnose, caused by infections of Glomerella cingulata, of many crops such as pepper, mango, banana and loquat and especially for preventing and treating the grape anthracnose, the strawberry anthracnose and other plant diseases caused by infection of Glomerella cingulata. The field test result shows that the fungicide has a good preventing and controlling effect on the grape anthracnose and the strawberry anthracnose, the bacterial strain fermenting technology is simple, and the production cost is low. The fungicide living spore content is high, and the storage quality is stable.

The invention relates to the application of trichoderma pseudokoningii SMF2 bacterial strains in plant growth promotion, and belongs to the technical field of microorganism application. According to the technical scheme, plant growth is promoted according to the following the steps of firstly, preparing a trichoderma pseudokoningii SMF2 microbial agent into a solution which is 100 times the concentration of the trichoderma pseudokoningii SMF2 microbial agent and conducting seed soaking processing; secondly, conducting root dipping processing when trichoderma pseudokoningii SMF2 microbial agent seedlings are transplanted; thirdly, evenly mixing the trichoderma pseudokoningii SMF2 microbial agent with organic fertilizer or fine sandy soil according to the ratio of 1:10 and conducting basal application processing. The trichoderma pseudokoningii SMF2 microbial agent can effectively promote the germination of seeds, the growth of the seedlings and the yield and the quality of crops, and has good application potential in the agricultural production process.

The invention discloses a polysaccharide from Trichoderma pseudokoningii, which is characterized in that (1) the high performance gel filtration chromatography test shows that the polysaccharide has a symmetrical peak with 18325 molecular weight; (2) an improved sulfuric acid-phenol method and a 3-aminophenol method test show that the neutral polysaccharide content is 65.2 percent, while the uronic acid content is 32.6 percent; (3) the GC analysis of the alditol acetate shows that the monosaccharide components are rhamnose, glucose and galactose, the molar ratio is Rha:Glc:Gal relates to 5.6:2.7:1.0, while when the polysaccharide is completely reduced, the molar ratio is Rha:Glc:Gal relates to 14.5:9.3: 1.0, having 26.6 molar percent glucuronic acid. The preparation method of the polysaccharide from Trichoderma pseudokoningii consists of the crude polysaccharide preparation and the polysaccharide purification. The polysaccharide from the Trichoderma pseudokoningii has broad applications in preparing the drugs or functional food for improving the mammal immunocompetence and for treating or adjuvant treating the tumors.

The invention relates to an application of Trichoderma pseudokoningii extracellular polysaccharides having anticancer activities. The Trichoderma pseudokoningii extracellular polysaccharides having the anticancer activities can reduce the vitality of K562 cells in vitro when they are applied to tumor growth inhibition (auxiliary) medicines, so intracellular active oxygen and free calcium ion concentrations are improved, the expression abundance of mRNA of p53 and Bax genes is improved, the expression of the Bc1-2 transcription level is reduced, the ectropion of cell membrane phosphatidylserine of the human chronic granulocyte leukemia cells K562 is induced, and the nuclear polycondensation and the fragmentation of K562 are induced to generate apoptotic bodies and apoptosis; and in-vivo administration can increase weights of tumor bearing mice and alleviate growth speeds and weights of tumors of the tumor bearing mice, thereby tumor cell killing and tumor growth inhibiting purposes are reached.

The invention relates to a novel method for extracting fulvic acids from industrially-produced waste edible fungi residues by fermentation. The novel method includes the steps of (1) preparing a primary seed solution; (2) preparing a secondary seed solution; (3) inoculating the secondary seed solution, bacillus subtilis, candida tropicalis and trichoderma pseudokoningii into a 1000-2000L of fermentation tank respectively and fermenting for 10-15 days so as to obtain a fermented seed solution; (4) treating pleurotus ostreatus residues; (5) inoculating the fermented seed solution into the pleurotus ostreatus residues to be inculated, and fermenting at the temperature 20-40 DEG C aerobically for 4-5 days so as to obtain fermented fungi residues; (6) extracting the fulvic acids; (7) performing spray drying so as to obtain flavescent fulvic acid powder. The fulvic acid content can be above 35% after fermentation, and yield of crude fulvic acids can be up to about 30% (dry matter percentage) through extraction.

The invention discloses an unpolluted, green and high-efficiency composite biological soil modifier which comprises the following components: trichoderma pseudokoningii fermentation liquor, bacillus subtilis fermentation liquor, water-soluble chitin, potassium humate and decomposed organic matter. The trichoderma pseudokoningii and the bacillus subtilis are high-efficiency bacterial strains which are obtained through screening and mutating, the trichoderma pseudokoningii fermentation liquor and the bacillus subtilis fermentation liquor are obtained by a liquor submerged fermentation technology, and the unpolluted, green and high-efficiency composite biological soil modifier can be prepared in a combination way. The soil modifier has the following functions that the physicochemical property of the soil can be improved, the hardening of the soil can be broken up, the volume weight of the soil can be reduced, the air permeability of the soil can be improved, the microbiological activity of the soil can be promoted, the growth of the plant root system can be promoted, the disease resistance of the crop can be enhanced, the yield of the crop can be improved, the quality of the crop can be improved, the original ecology of the crop can be recovered and the like, and the soil modifier is the novel soil modifier which is high-efficiency, environment-friendly and convenient to use.

The invention relates to a preparation method and application of total nutrient microorganism biocontrol organic fertilizer. The preparation method of the total nutrient microorganism biocontrol organic fertilizer comprises the following steps: firstly, carrying out plate cultivation on trichoderma pseudokoningii TRIPI and rhizopus nigricans RN-9; secondly, carrying out PDB liquid culture on TRIPI and RN-9 hypha blocks; thirdly, carrying out fermentation culture on a liquid culture solution, so that fermentation liquor is obtained; fourthly, inoculating the fermentation liquor to farmyard manure raw materials, uniformly mixing, stockpiling and fermenting, so that a fermented material is obtained; fifthly, adding other ingredients into the fermented material, and uniformly mixing, so that the total nutrient microorganism biocontrol organic fertilizer is obtained. The prepared total nutrient microorganism biocontrol organic fertilizer has high inhibition ratio on pathogenic bacteria and high relative control efficiency on fusarium wilt of cucumber; compared with biocontrol organic fertilizer prepared by adopting single strain fermentation liquor, content of pathogenic bacteria in cucumber rhizosphere soil, cucumber root system and cucumber stalk is low, and cucumber blade protective enzyme is quick in response, high in activity and long in duration of time.

Disclosed is an invention that relates to the formation of microsclerotial propagules by mycoparasitic fungi and the use of those microsclerotia for plant disease control. Representative microsclerotia propagules formed are from fugal species Trichoderma harzianum, Trichoderma lignorum, Trichoderma viridae, Trichoderma reesei, Trichoderma koningii, Trichoderma pseudokoningii, Trichoderma polysporum, Trichoderma hamatum, and Trichoderma asperellum.

The invention relates to trichoderoma koningii TC-72 and application of the trichoderoma koningii TC-72 to biological control of aspergillus flavus. The trichoderoma koningii TC-72 is preserved in the China Center For Type Culture Collection on January 7th, 2016, and the preservation number is CCTCC NO:M 2016015. The trichoderoma koningii TC-72 is obtained by being separated out of peanut pods of Babu Town, Hezhou City, Guangxi Province. It is found through indoor antagonistic experiments, living body bacteriostatic detoxification experiments and field controlling experiments that a bacterial strain is high in bacteriostatic ability, good and stable in bacteriostatic detoxification effect, easy to culture, free of pollution and safe to environment. After bacterial manure is applied to soil, antagonistic bacteria can be fast reproduced on the rhizosphere of crops and survive for a long time, a dominant bacterial community is formed, and harm of aspergillus flavus to peanuts can be effectively controlled.

The invention discloses a microbial preparation and application thereof and belongs to the technical field of microorganisms. According to the microbial preparation disclosed by the invention, agricultural microorganisms of photosynthetic bacteria, nodule bacteria, trichoderma pseudokoningii and the like and lactobacillus plantarum which can generate a synergistic effect with growth and metabolismof the agricultural microorganisms are utilized and compounded to prepare a safe and pollution-free microbial inoculum. The prepared microbial preparation disclosed by the invention has important application potential in the food field to prepare products with pepper as a main raw material or the agricultural planting field.

The invention discloses preparation and an application method of a special microorganism composite bacterial agent for directly decomposing and fermenting crop straws to generate marsh gas. The special microorganism composite bacterial agent is liquid or solid, and is formed by fermenting, culturing, combining and compositing of one or more of bacillus cereus, bacillus subtilis, bacillus licheniformis, bacillus megaterium, bacillus mucilaginosus, bacillus circulans, bacillus sphaericus, clostridium sporogenes, clostridium acetobutylicum, clostridium pasteurianum, clostridium beijerinckii, clostridium thermocellum, trichoderma viride, trichoderma pseudokoningii and aspergillus niger. Meanwhile, by taking the special microorganism composite bacterial agent as an inoculation bacterial agent,the invention provides the application method for decomposing the crop straws and accelerating the fermentation to generate marsh gas; the crop straws treated by the microorganism composite bacterialagent have fast fermentation speed, large marsh gas generation quantity, low treating cost and no secondary pollution, can be fermented to generate the marsh gas under the condition of no any faeces of poultry and livestock, and the application range is quite wide.

The invention discloses a Chinese medicament group for assisting chemotherapy, relating to the field of medication. The Chinese medicament group comprises trichoderma pseudokoningii extracellular polysaccharide, astragalus root, red ginseng, Chinese angelica root, dwarf lilyturf turber, suberect spatholobus stem, sesame, common yam rhizome, lotus plumule, licorice root, lucid ganoderma and spreading hedyotis herb. The Chinese medicament group is prepared according to treatment principles of safety, reliability, healthy energy supporting of the traditional Chinese medicine, blood activation, heat clearing and the like, and can reduce adverse use effects according to regulation of medicament properties among the medicaments, and meanwhile, the Chinese medicament group can solidify the fundamental essence of life to ensure that both qi and yin are invigorated and nourished, and can also inhibit the rapid development of tumors and treat both symptoms and root causes, so that the Chinese medicament group can be taken for a long time.

The invention discloses application of trichoderma pseudokoningii exopolysaccharide in preventing and treating colon cancer and application of a chemotherapeutic drug combined with the trichoderma pseudokoningii exopolysaccharide in treating the colon cancer. The trichoderma pseudokoningii exopolysaccharide (EPS) can significantly inhibit the growth of tumor masses in a mouse with the colon cancer, and can improve mouse immune organ index and the levels of interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-alpha) in mouse serum, increase the proportion of mouse cytotoxic T lymphocytes (CD8+T cells) in total T lymphocyte calculation and significantly activate mouse T lymphocytes and B lymphocytes; therefore, the EPS has an effect on treating the colon cancer; and the EPS, when taken for a preventive purpose, can also effectively improve the antitumor capacity of a body. In addition, the EPS, when combined with an existing chemotherapeutic drug, namely 5-fluorouracil (5-FU), is significantly better than the single medication of the 5-FU in antitumor effect. Moreover, the EPS can improve the life quality of the mouse with the colon cancer and can prolong the service life of the mouse.

The biological and biochemical preparation is a kind of multiple target site preparation containing Trichoderma pseudokiningii and Bacillus spp as well as cell membrane protecting matter, composite inductor, natural antibiotic matter, such as trace elements, and beneficial matters, such as taurine, vitamin C, etc. Trichoderma pseudokiningii is strain obtained through separating and screening stalk and named as TRIP1, and Bacillus spp is separated from soil and named as BAC1. The biological and biochemical preparation may be used in preventing and controlling several kinds of plant diseases, promoting growth of plant, protecting cell membrane and raising plant photochemical efficiency.

The invention discloses mixed fermentation liquid inducing botrytis cinerea resistance and a preparation method of the mixed fermentation liquid. The mixed fermentation liquid is prepared by mixed fermentation of rhizopus nigricans with a preservation number of CGMCC NO. 8436 and trichoderma pseudokoningii with a preservation number of CGMCC NO. 1443. The mixed fermentation liquid can obviously improve induced resistance of tomatoes against botrytis; the rhizopus nigricans and the trichoderma pseudokoningii used in the mixed fermentation liquid are derived from a natural environment and can effectively induce the systematic resistance of the tomatoes against botrytis cinerea; a use method of the mixed fermentation liquid is simple and convenient; the mixed fermentation liquid has no environment pollution and is nontoxic to human and livestock; a production method of the fermentation liquid is simple and convenient and short in period; the used raw materials are cheap; large-scale massive production is easy; the production cost is low.

The invention provides a magnetic biological fertilizer. The magnetic biological fertilizer comprises the following raw materials in parts by mass: 200-400 parts of nitrate mineral, 200-400 parts of plant stalk, 150-250 parts of soybean molasses, 100 parts of plant ash, 100 parts of pulverized fuel ash, 20 parts of composite strain and 10 parts of trace element addition solution. The nitrate mineral comprises the following nutrient components: greater than 30% of silicon dioxide, greater than 20% of calcium carbonate, greater than 12% of iron element, greater than 3% of magnesium element and greater than 5% of potassium element. The trace element addition solution comprises boron element, zinc element, molybdenum element, selenium element and the like. The composite strain comprises trichoderma pseudokoningii and bacillus subtilis. Through the magnetic biological fertilizer, the soil structure can be effectively improved; breathability and water permeability of the soil can be enhanced; nutrients in the soil are increased; adsorption quantity of organic matters is large; the nutrients are not liable to loss, easy to decompose and easy to absorb; the nitrate mineral, the biological fertilizer technique and the magnetic technology are effectively integrated; the magnetic biological fertilizer is complete in nutrients, strong in functionality, stable in physical and chemical indexes and excellent in mutual promotion effect.

The invention discloses application of a compound biological agent in promoting quality of vegetable products. The compound biological agent is prepared from bacillus subtilis fermentation liquor, trichoderma pseudokoningii fermentation liquor, water-soluble chitin, aginomoto residue and potassium fulvic acid. An experimental result proves that the strawberry treated with the compound biological agent, compared with the control group, has the advantages of obviously increased weight of single fruit, soluble solids, soluble sugar, titratable acid content and solid acid ratio. The invention aimsat researching the practice feasibility and production operability; the compound biological agent meeting the national standard for organic products is applied in the growth season of the strawberry;the compound biological agent is sprayed onto the leaves at regular intervals according to the growth characteristics of the strawberry, so that the purpose of improving the strawberry quality is achieved. The probiotic has a certain function of preventing and controlling the crop plant diseases, so that a planter is willing to use the method; no additional labor cost or equipment cost is added;the quality of the vegetable products is increased; the operation is easily mastered; the popularization is strong.

The invention discloses a straw decomposition microbial inoculant and application thereof. The straw decomposition microbial inoculant disclosed by the invention is composed of the following active components: Aspergillus niger, Trichoderma pseudokoningii, Penicillium viridicatum, xylan-solubilizing clostridium, pseudomonad and Bacillus subtilis. The straw rotten microbial inoculant has quick decomposition effect on crop straws. After the straw rotten microbial inoculant is applied for 7 days, the total decomposition rates of the wheat straws and rice straws are respectively up to 25.6% and 28.9%; and after 10 days, the total decomposition rates of the corn straws and rape straws are respectively up to 30.8% and 23.5%. After the straw rotten microbial inoculant is applied for 15 days, the total decomposition rates of the wheat straws and rice straws are respectively up to 60.1%, and the total decomposition rates of the corn straws and rape straws are respectively up to 63.8% and 57.7%.

The present invention relates to a strain Trichoderma pseudoconii ( Trichoderma koningiopsis ) TZ-11 strain, application and preparation method thereof. The wettable powder prepared by the strain through solid fermentation can be used to prevent and control the bacteria caused by the small clusters of bacteria ( Glomerella cingulata ) infection caused by anthracnose, such as capsicum, mango, banana and loquat and many other crop anthracnose control, especially by the small clusters of bacteria ( Glomerella cingulata ) infection caused by grape anthracnose and strawberry anthracnose and other diseases. Field test results showed that the fungal agent had good control effects on both grape anthracnose and strawberry anthracnose, and the strain fermentation process was simple, and the production cost was low; the fungal agent had high content of live spores and stable storage properties.

The invention discloses a composite bio-formulation for preventing crop diseases, comprising the following raw materials: trichoderma pseudokoningii fermenting liquid, bacillus subtilis fermenting liquid, water-soluble chitosan, sodium glutamate residue liquid and potassium humate, wherein the trichoderma pseudokoningii and the bacillus subtilis are high-effect strains which are obtained after being screened and processed, later the fermenting liquid of the trichoderma pseudokoningii and the bacillus subtilis is obtained through a submerged fermentation technique, and the composite bio-formulation for preventing crop diseases is obtained through combining. The composite bio-formulation for preventing crop diseases in the invention has obvious effects for preventing blight, verticillium, gray mold, phytoph-thora capsici leonian, downy mildew, powdery mildew, gray mold, fulvia fulva and so on, and can be especially used for preventing the blight and the verticillium which have large danger in agriculture. The invention is suitable for preventing the diseases and improving yields of various vegetables, melons, fruits and crops, such as tomatoes, chili peppers, eggplants, cucumbers, watermelons, apples, Chinese chives, cottons and so on.