67 results about "Trichoderma aureoviride" patented technology

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, is used for improving soil / land and especially can be used for effectively improving soil polluted by heavy metals.

The invention discloses a crop straw decomposing inoculant and application in straw bioreactor and water-logged compost. The inoculant is compounded of 0.5-2 parts of phanerochaete chrysosporium, 0.5-2 parts of trichoderma aureoviride, 0.5-2 parts of bacillus subtilis and 0.5-2 parts of bacillus mucilagimosus krassilm, wherein the effective viable count is 6*10<8> per gram. The straw decomposing inoculant is applied to a tomato stalk bioreactor planting technology, and the average ground temperature is 18.3 DEG C and is increased by 2.2 DEG C as compared with that of contrast without use of the inoculant; the average greenhouse temperature is 14.3 DEG C and is increased by 2.0 DEG C as compared with the average greenhouse temperature of the contrast; the average CO2 concentration is 956ppm and is 2.17 times higher than that of the contrast; the harvest time is 15 days earlier than the harvest time of the contrast; the acre yield is 5881kg and is increased by 22.7 percent as compared with the acre yield of the contrast; the occurrence rate of plant diseases and insect pests is lower as compared with that of the contrast. The inoculant is applied to wheat straw retting and decomposing, is high in decomposition speed, high in compost flexibility, the straws are basically decomposed, and the fermentation time is shorter than that of an inoculant purchased in the market.

The invention provides a special biological organic fertilizer for iron-rich green vegetables as well as a preparation method and an application of the fertilizer. The organic fertilizer is prepared from the following components in parts by weight: 60-80 parts of edible mushroom residues, 15-35 parts of humic acid, 5-15 parts of rapeseed dregs, 5-10 parts of iron in a chelating state and ferric citrate and 0.2-2 parts of a compound microbial agent. By using edible mushroom residues, humic acid and rapeseed dregs as raw materials, adding trace element iron and taking bacillus amyloliquefaciens and trichoderma aureoviride as special effect microbial bacteria, the biological organic fertilizer provided by the invention has high fertilizer efficiency and has multiple functions of improving physical and chemical properties of soil, effectively reducing soil-borne disease, improving the crop output and nutritional quality and the like.

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, can survive for a long term in a plurality of improved soil to play a role, is good in adaptation of improving soil / land and especially can be used for effectively improving a plurality of soil, such as secondary salinization land, desertification soil.

The invention relates to the technical field of crop fertilizers, and particularly relates to a special stubble-resistant biological bacterial fertilizer for radix salviae miltiorrhizae. The special stubble-resistant biological bacterial fertilizer comprises the following components in percentage by weight: 8%-12% of microbial agents, 40%-50% of fresh pepper seeds, 40%-50% of organic substances and 2%-5% of trace elements. According to the special stubble-resistant biological bacterial fertilizer, the problems of serious blight, root rot and root-knot nematode disease, which are caused due to years of continuous cropping, of the radix salviae miltiorrhizae are well solved, so that the output and quality of the radix salviae miltiorrhizae are increased; the double roles of sterilization and protection are played by adopting a trichoderma aureoviride W2256 strain, so that the soil is improved, and the environment is protected; the addition of a pure natural organic fertilizer is favorable to the growth of trichoderma, and the pure natural organic fertilizer and the trichoderma complement each other; a raw material is a pure biological raw material without pollution on the environment and crops, so that the content of heavy metals contained in the soil can be effectively reduced, and the content of organic matters contained in the soil can be increased; in addition, the reasonable formula, equivalent nutrition and better using effect are achieved, the utilization ratio of the fertilizer can be obviously enhanced, and the output and quality of the radix salviae miltiorrhizae can be increased.

The invention discloses a preparation technology of fly maggot chitin and chitosan and an application of fly maggot chitin to inducing a trichoderma aureoviride T2 bacterial strain to generate chitinase, belonging to the field of biological technology. The preparation technology of fly maggot chitin has the main characteristics that the traditional hydrochloric acid calcium removal technology is changed into an EDTA (Ethylene Diamine Tetraacetic Acid) calcium removal technology, the environmental pollution caused by waste acid discharge can be reduced, the damage of concentrated acid on chitin molecular chains can be avoided, and the chitin product with the high quality is obtained; and according to the preparation technology of fly maggot chitin, the traditional technology of 4-hour high-temperature concentrated alkaline refluxing is changed into a sodium hydroxide-amyl alcohol reaction system, microwave heating treatment is carried out twice, the utilization rate of alkali liquid is increased, the reaction time is greatly shortened, the energy consumption is saved, and a white powdery chitosan product with the lower moisture content and the higher degree of deacetylation is obtained. The fly maggot chitin disclosed by the invention can be used as a carbon source for inducing the trichoderma aureoviride T2 bacterial strain to generate the chitinase and has obvious induction effect.

The invention discloses preparation processes of flyblow chitin and chitosan and application of the flyblow chitin in inducing trichoderma aureoviride T2 strains to produce chitinases, belonging to the technology field of biology. In the preparation process of the flyblow chitin, the traditional hydrochloric acid calcium removal process is mainly changed to an ethylene diamine tetraacetic acid (EDTA) calcium removal process, thereby reducing the environmental pollution caused by waste acid emission, avoiding the damage of concentrated acid to chitin molecular chains and obtaining the high-quality chitin product; and in the preparation process of the flyblow chitosan, the traditional process in which high-temperature concentrated alkali is refluxed for 4 hours is changed to a process in which a sodium hydroxide-amyl alcohol reaction system is adopted and microwave heating treatment is carried out twice, thereby improving the utilization of an alkali liquid, greatly shortening the reaction time, reducing energy consumption and obtaining the white powdery chitosan product with low water content and high deacetylation degree. The flyblow chitin provided by the invention can also be used as a carbon source so as to induce the trichoderma aureoviride T2 strains to produce the chitinases, wherein the induction effect is obvious.

The invention belongs to the field of microorganisms and microorganism application, and in particular relates to a trichoderma aureoviride strain TW351 (Trichodermaaureoviridesp.) and application of the trichoderma aureoviride strain in inhibition against plant diseases. The strain is stored in China Center for Type Culture Collection in Oct., 17th in 2012, and the preservation number is CCTCC NO:M2012407; the strain can quickly grow on a personal digital assistant (PDA) solid culture medium under the temperature of 28 DEG C; after 48 hours, the radius of a bacterial colony is 3.4 cm; after 72 hours, the radius of the bacterial colony is 5.4 cm; the form of the strain under an optical microscope is that formed mycelium is white or colorless and is in a short fur type; after being matured, the bacterial colony is light yellow green. When ginger seeds are planted, the seed gingers are soaked in a trichoderma preparation according to the proportion of the preparation to water of 1:100; and therefore, the ginger damping off disease prevention rate is up to 82 percent. When the damping off diseases are prevented, effects for accelerating germination of the ginger seeds and hestening the rooting of the ginger seeds can be achieved.

The invention relates to a trichoderma aureoviride particle adsorbent for treating uranium-bearing wastewater as well as a preparation method and application of the absorbent, and belongs to the technical field of production and manufacturing of a sewage treatment material. The adsorbent is prepared from trichoderma aureoviride fungus purely-cultured viable bacteria, corncob particles and an embedding agent and is 4mm-6mm in particle size; the preparation method comprises the following steps: culturing the trichoderma aureoviride bacteria and washing with sterile water to obtain conidium suspension liquid; washing, drying and crushing corncobs, and then sieving the particles of which the particle size is 1mm-2mm; and adsorbing, embedding, culturing and freeze-drying conidiums to obtain the trichoderma aureoviride particle adsorbent. The application is as follows: the trichoderma aureoviride particle adsorbent is added into a uranium-bearing water solution, and then the vibration and the adsorption are performed. The particle adsorbent prepared by adopting the method disclosed by the invention is low in cost, simple and convenient to operate, free of secondary pollution, liable to separate from the water solution, reusable, and capable of being used for effectively adsorbing and recycling uranium ions in the water solution.

The invention discloses composite microbial fluid fertilizer. The composite microbial fluid fertilizer is prepared by using biogas fluid obtained by carrying out anaerobic fermentation on hen manures of laying hens as a main raw material, compounding urea, potassium dihydrogen phosphate and microbial flora, and then mixing, dissolving, filtering, weighing and packing; the microbial flora is prepared from bacillus subtilis microbial agent and trichoderma aureoviride; the composite microbial fluid fertilizer is mainly prepared from the following raw materials in percentage by weight: 89.3 to 91.8 percent of biogas fluid obtained by carrying out anaerobic fermentation on the hen manures of the laying hens, 4 to 5 percent of the urea, 4 to 5 percent of potassium dihydrogen phosphate, 0.3 to 0.4 percent of the bacillus subtilis microbial agent and 0.2 to 0.3 percent of the trichoderma aureoviride. The invention also provides a preparation method of the composite microbial fluid fertilizer. The composite microbial fluid fertilizer disclosed by the invention is higher in fertilizer efficiency and security; the activity of soil microorganism can be quickly improved, heavy metal is degraded, soil-borne diseases are inhibited, and an indelible effect on development of crop roots and prevention and control of plant diseases and insect pests is achieved; moreover, the composite microbial fluid fertilizer can be widely applied to water and fertilizer integrated planting on crops such as a variety of grain crops, economic crops, and fruits and vegetables, and also can be used as spraying and water-jetting fertilizer applied to foliage.

The invention belongs to the technical field of vegetative propagation of plants, and particularly relates to an accelerant facilitating plant cutting rooting.The accelerant is prepared from 4 parts of sinorhizobium fredii fermentation liquor, 3 parts of trichoderma aureoviride fermentation liquor, 4 parts of candida albican fermentation liquor, 3 parts of bacillus licheniformis fermentation liquor, 2 parts of gibberellin, 5 parts of grass carbon and 2 parts of dipotassium phosphate.The accelerant is a nutritional preparation special for asexual cutting rooting of torreya jackii, a good microecosystem can be formed, plant growth is promoted, beneficial microbiological florae are formed, nutrition environments of plant rhizosphere are improved, cutting rooting and the survival rate are greatly promoted, and an application prospect is achieved.

The invention provides a new method for preparing fermentation feed with a health-care function for cows and sheep. The method comprises the following steps: crushing sugarcane stalks by an ordinary straw crusher; mixing the following raw materials in parts by mass to obtain a mixture: the sugarcane stalks, vegetable dregs or peanut meal, bran and mineral substances; preparing microbial inoculum (which consists of trichoderma viride, trichoderma aureoviride and bacillus in the type of feed) for degrading stalks, and to prepare preparing fermentation composite liquid; sealing the fermentation composite liquid in a fermenting tank for more than 14 days for fermenting, to obtain the feed with the health-care function for cows and sheep. The method disclosed by the invention uses crop straws of which the resources are rich and food processing by-products as main coarse feed raw materials, and studying and developing the new technology of the fermentation feed with the health-care function, comprehensive nutrition, high digestibility and good palatability have important realistic significance and practical value.

The invention discloses trichoderma aureoviride which can produce IAA (Indole Acetic Acid) and siderophores and is high in phosphate solubilization property. The trichoderma aureoviride is characterized by comprising applications of relieving cucumber seedling salt stress and prompting seedling grow, wherein specifically the contents of cucumber seedling chlorophyll, soluble sugar and the soluble protein are increased, the content of MDA (Methylene Aioxyamphet Amine) is reduced under the salt stress, and the phosphate solubilization capablility of the strain is one of the desalting and grow prompting mechanisms.

The invention relates to a preparation for treating aniline waste water and a treatment method thereof. The preparation is prepared from pseudomonas putida, candida albican, bacillus amyloliquefaciens, paper dissolving clostridium, nitrosobacteria, trichoderma aureoviride, nitrifying bacteria, denitrifying bacteria and a carrier. Compatibility among the strains is reasonable, the strains grow together, coordinate, are not antagonistic, are placed into a waste water treatment system, have a good effect of degrading aniline compounds difficult to degrade and are suitable for industrial sewage treatment, the water treatment capacity is increased, the water treatment quality is improved, the operating cost is reduced, and the waste water reaches the standard and is discharged.

The invention discloses a trichoderma aureoviride strain and a method for preparing a notoginseng bud cigarette fragrance by utilizing the same.The strain is trichoderma aureoviride HLMM-04 CGMCC No.10839, and has the capability of producing cellulase and promoting the decomposition of cellulose into oligosaccharide or monosaccharide.The strain undergoes activation, fermentation, filtration and dialysis to prepare cellulase crude enzymes, the cellulase crude enzymes are used for fermenting notoginseng buds, and the cigarette fragrance is prepared after extraction, filtration and decompress concentration.The optimal enzyme-producing conditions of the HLMM-04 strain are that the initial pH of a culture medium is 6.5, the culture temperature is 28 DEG C, and the culture time is 5 d.Cigarette perfuming results indicate that the prepared fragrance has obvious characteristic fragrance of the notoginseng buds, offensive odor is lowered, and the fragrance is mellower in taste and has obvious effects of engendering liquid and improving throat comfort.

The invention relates to a bioremediation preparation of aniline contaminated water. The bioremediation preparation is prepared from a compound bacterium agent and auxiliary materials. The compound bacterium agent is prepared from pseudomonas putida, candida mycoderma bacteria, bacillus amyloliquefaciens, clostridium, nitrosomonas, trichoderma aureoviride and nitrifying bacteria. The strains are reasonable in compatibility, coexist and coordinate with one another, and are not mutually antagonistic. If the preparation is put into a wastewater treatment system, a good degradation effect is achieved on aniline degradation-resistant compounds, the preparation is suitable for industrial sewage treatment, the amount of treated water is increased, the quality of the treated water is improved, operation cost is reduced, and up-to-standard discharge is promoted.

The invention discloses a biocontrol strain for preventing and treating poplar canker and an application method thereof. The strain is trichoderma aureoviride, with number of CGMCC No.8891. Fermentation liquor of the strain is mixed with wheat bran and corn powder, the product is naturally air-dried and ground to prepare raw powder after fermentation for seven days, and 38wt% of raw powder, 38wt% of kaolin, 13wt% of sodium lignosulphonate, 8.5wt% of SDS and 2.5wt% of humic acid are added to prepare an agent. After being diluted, the agent is used for preventing and treating the poplar canker by spraying and root irrigation. The strain has the advantages of various modes of action on pathogenic bacteria, high reproduction speed, stable action effects, and the like, has strong inhibiting effects on the pathogenic bacterium botryosphaeria dothidia of the poplar canker, is of great importance in prevention and control of the poplar canker and has good development and application prospects.

The invention discloses a trichoderma aureoviride strain and a method for preparing fig cigarette flavor with the same. The strain is trichoderma aureoviride HLMM-04 CGMCC No.10839 and has capacities of producing cellulase and further promoting cellulose to be decomposed into oligosaccharide or monosaccharide. The strain is subjected to activation, fermentation, filtration and dialysis, and a crude cellulase liquid is prepared and used for fermenting figs, extraction, filtration and vacuum concentration are performed, and the cigarette flavor is prepared. The optimum cellulase producing conditions of the HLMM-04 strain are as follows: the initial pH of a medium is 6.5, the culture temperature is 28 DEG C, and the culture time is 5d. Results of cigarette flavoring prove that the prepared flavor has the advantages that the aroma richness is increased and the fruit sweetness is more outstanding while original characteristic aroma of the figs is maintained.

The invention belongs to the technical field of biological pesticide, and particularly relates to a plant immune resistant inducer and application thereof. The plant immune resistant inducer is prepared by using a Trichoderma aureoviride T2 protein elicitor (TraT2A) which is adopted as a main effective active ingredient and a surfactant, a thickener, a stabilizer, an antifreeze and an antifoamingagent at a specific ratio. The prepared plant immune resistant inducer can be applied to induction of supply of enzyme activities related to disease resistance of lily, the disease resistance of lilycan be enhanced, and the preparation has no pollution to the environment and no harm to human beings and animals, and has good pesticide efficacy.

The invention discloses a method for preparing fermented flavor for tobacco with seedless roxburghii. Wash the seedless prickly pears, squeeze them, and filter to separate the juice from the residue. Pectinase and glucoamylase were used to enzymatically hydrolyze the juice of Rosa roxburghii, and Trichoderma chrysogreen HLMM‑04 strain was used to prepare crude enzyme solution, and the residue of Rosa roxburghii was fermented. The juice after enzymolysis is mixed with the residue after fermentation, extracted by reflux, filtered to remove residue, and concentrated under reduced pressure to obtain the seedless prickly pear fermented spice. The preservation number of the Trichoderma chrysogreen HLMM‑04 strain is CGMCC No. 10839, which has the ability to produce cellulase. The prepared fermented flavor for tobacco has obvious fruity aroma, is well coordinated with smoke, and has the functions of increasing the richness of smoke and improving taste.

The invention belongs to the field of saline-alkali land plant planting, and relates to a saline-alkali land crop planting method. According to the method, after fertilizer application, horizontally-arranged {-shaped cultivation is performed, wherein the width of the zone is 70-110 cm, a small ditch with a width of 8-12 cm and a depth of 8-12 cm is formed in the middle of the sowing zone, ridges with a height of 10-20 cm and a bottom width of 15-25 cm are formed on the edge sides, the row spacing is 35-55 cm, the whole zone is subjected to mulching planting, both edge sides, having the width of 3-10 cm, of the zone are not subjected to mulching, and pores are drilled on a ditch bottom film every 3-8 cm in the small ditch, and are covered with soil. The invention further provides a specialfertilizer for the method, wherein the special fertilizer comprises: Trichoderma aureoviride, wheat bran, sulfur plus resin coated controlled release urea, calcium ammonium nitrate, calcium superphosphate, potassium sulfate, ferrous sulfate, and humic acid. According to the present invention, with the planting method, the salt content of the soil in the sowing zone is reduced; and by combining theplanting method and the special fertilizer, the microbial environment is improved, and the populations and the quantity of the rhizosphere microorganisms are increased so as to improve the peanut root development, such that the great significance is provided in the promotion of the production of oil crops such as peanuts and the like in saline-alkali land.

The invention relates to ecological organic fertilizer with a soil improving function. The ecological organic fertilizer with the soil improving function is prepared from the following components in parts by mass: 35-60 parts of organic fertilizer, 10-20 parts of urea, 4-12 parts of polyglutamic acid, 8-20 parts of humic acid, 5-9 parts of ammonium chloride, 10-15 parts of trichoderma aureoviride,20-30 parts of straw, 3-5 parts of iron ore powder, 9-15 parts of waste tobacco leaf dottle, 20-30 parts of fishpond sludge and 6-10 parts of bacillus subtilis. By means of the ecological organic fertilizer with the soil improving function, a soil structure can be improved, a proper ratio of large pores to small pores of soil is guaranteed, soil ventilation is improved, the effects of removing and preventing soil hardening are obvious, and meanwhile, the ecological organic fertilizer with the soil improving function has huge ion exchange and adsorption capability, high water absorption, waterretaining and fertilizer retaining capability, and good adsorption and buffering effects on acid-causing ions and alkali-causing ions, so that the ecological organic fertilizer has a good effect of improving the soil.

The invention discloses a biocontrol Bacillus thuringiensis strain and an application thereof. The collection number of Bacillus thuringiensis BCN10 is CGMCC No: 16675. Fermentation liquor of the Bacillus thuringiensis effectively inhibits common loquat surface putrefying fungi (fusarium oxysporum, botryosphaeria parva, trichoderma aureoviride, colletotrichum gloesporioides and penicillium expansum) on a bi-zone flat plate and a loquat fruit. Volatile substances generated by the fermentation liquor are applied to corrosion resistance in loquat storage, natural putrefaction disease time of theloquat fruit can be delayed, and incidence rate is decreased. Volatile antibacterial substances generated by the Bacillus thuringiensis BCN10 can avoid direct contact with the fruit, the fruit is preserved at normal temperature, food storage time is prolonged, and the strain can be used for preparing a biocontrol preservative which is used for inhibiting the putrefying fungi such as the fusarium oxysporum, the botryosphaeria parva, the trichoderma aureoviride, the colletotrichum gloesporioides and the penicillium expansum and has a wide antibacterial spectrum.

The invention discloses a straw fiber seedling-raising substrate for colonizing biocontrol bacteria, a production method of the straw fiber seedling-raising substrate, and a two-step biocontrol bacteria colonizing method of the straw fiber seedling-raising substrate, and belongs to the technical field of crop colonization biocontrol bacteria. Specifically the two-step biocontrol bacteria colonizing method comprises the following steps: the first step is the colonization of a biocontrol bacteria powder mixed with the substrate, the spray colonization of a biocontrol bacteria water agent, or thespray colonization of a biocontrol bacteria liquid; and the second step is that 10-15 days after the straw fiber substrate colonization biocontrol bacteria, the straw fiber substrate colonized with biocontrol bacteria, and crop seedlings are transplanted into a field simultaneously, and the substrate and crop seedling biocontrol bacteria are colonized in the field, wherein the used biocontrol bacteria are one or a mixture of more than one of Trichoderma aureoviride, Bacillus amyloliquefaciens, Streptomyces parvus, Talaromyces flavus, and Chaetomium aureum. According to the straw fiber seedling-raising substrate for colonizing biocontrol bacteria, the production method of the straw fiber seedling-raising substrate, and the two-step biocontrol bacteria colonizing method of the straw fiber seedling-raising substrate, the biocontrol bacteria can be effectively colonized, thereby the disease resistance of the seedlings is improved, and the stress resistance of seedling transplanting is significantly improved.

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, is used for improving soil / land and especially can be used for effectively improving soil polluted by heavy metals.

The invention discloses a trichoderma aureoviride strain and method for preparing a Pu'er Tea tobacco flavor. The strain is trichoderma aureoviride HLMM-04 strain (CGMCC No .10839), and has the abilities to produce cellulase and then promote cellulose decomposition into oligosaccharide or monosaccharide. The strain is made into cellulase crude enzyme liquid through activation, fermentation, filtration and dialysis, the cellulase crude enzyme liquid is used for fermenting Pu'er Tea, then, extraction, filtration and concentration under reduced pressure are conducted, and the tobacco flavor is obtained. The optimum enzyme producing conditions of the HLMM-04 strain are as follows: the initial PH value of a culture medium is 6.5, the culture temperature is 28 DEG C, and the culture time is 5 days. The cigarette flavoring results show that the prepared flavor has the effects of improving smoking aroma quality, increasing aroma amount, and improving the aftertaste.

The invention discloses a trichoderma aureoviride HLMM-04 strain and a method for preparing a tobacco flavor.The strain is trichoderma aureoviride HLMM-04 CGMCC No.10839, and has the capacity of producing cellulase and promoting decomposition of cellulase into oligosaccharide or monosaccharide.The strain is subjected to activation, fermentation, filtering and dialysis and prepared into cellulase crude enzyme liquid which is used for fermenting a mixture of loquat, loquat leaves, lilies, hawthorn fruit and boat-fruited scaphium seeds, and then the mixture is subjected to extraction, filtering and vacuum concentration to be prepared into the tobacco flavor.According to the most appropriate enzyme production conditions of the HLMM-04 strain, the culture medium initial pH is 6.5, culture temperature is 28 DEG C, and culture time is 5 d.A flavoring result shows that the prepared flavor has the effects of reducing irritation and improving the comfort degree of the throat, and has remaining taste.

The invention discloses a trichoderma aureoviride strain and a method for preparing a panax notoginseng cigarette fragrance by utilizing the same.The strain is trichoderma aureoviride HLMM-04 CGMCC No.10839, and has the capability of producing cellulase and promoting the decomposition of cellulose into oligosaccharide or monosaccharide.The strain undergoes activation, fermentation, filtration and dialysis to prepare cellulase crude enzymes, the cellulase crude enzymes are used for fermenting panax notoginseng, and the cigarette fragrance is prepared after extraction, filtration and decompress concentration.The optimal enzyme-producing conditions of the HLMM-04 strain are that the initial pH of a culture medium is 6.5, the culture temperature is 28 DEG C, and the culture time is 5 d.Cigarette perfuming results indicate that the prepared fragrance has obvious panax notoginseng characteristic aroma and is light in bitterness and astringency and comfortable in taste.

The invention discloses a Trichoderma chrysanthemum HLMM‑04 strain and a method for preparing tobacco spices. The strain is Trichoderma aureoviride HLMM-04 CGMCC No.10839, which has the ability to produce cellulase, and then promote the decomposition of cellulose into oligosaccharides or monosaccharides. The cellulase crude enzyme solution is obtained by activating, fermenting, filtering and dialysis of the bacterial strain, which is used to ferment the mixture of loquat, loquat leaf, lily, hawthorn and prawn sea, and then extracted, filtered and concentrated under reduced pressure to make tobacco flavor. The optimum conditions for enzyme production of HLMM‑04 strain were as follows: the initial pH of the medium was 6.5, the culture temperature was 28°C, and the culture time was 5 days. The results of perfuming showed that the prepared spices could reduce irritation, improve throat comfort and aftertaste.