Trichoderma pseudokoningii (SMF2) strain and application thereof

A technology of Trichoderma pseudokoningii and strains, applied in the field of microorganisms, can solve problems such as research lag, and achieve the effects of huge quantities, reduced dosage, and wide sources

Inactive Publication Date: 2011-05-25
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, my country's research on Trichoderma biocontrol lags behind and urgently needs to be strengthened.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040]A strain of Trichoderma pseudokoningii SMF2 (Trichoderma pseudokoningii SMF2) was preserved in the China Center for Type Culture Collection on February 24, 2009, with the preservation number CCTCC NO: M209031.

[0041] The above-mentioned Trichoderma pseudokoningeni SMF2 can grow rapidly with cellulose as the sole carbon source, and can rapidly grow and colonize in soil with (hemi)cellulose as the carbon source, decompose organic matter in the soil, and promote plant growth. And the strain high-yielding fungal cell wall degrading enzymes (CWDEs) and antibiotics - peptaibols (peptaibols). Thereby, they can play a synergistic role and improve the effect of biological control.

Embodiment 2

[0043] The steps of expanding the cultivation of Trichoderma pseudokoningeni SMF2 are as follows:

[0044] (1) The preparation method and activation conditions of the activation medium are as follows:

[0045] Preparation method of activated medium:

[0046] Mix the treated potato pieces with 4 parts by weight of water per part by weight, soak at 80°C for 1 hour, filter, and dilute the filtrate to the original volume with water; add 2 parts of glucose, 1.5 parts of agar, and the natural pH value of 8 sterilized to obtain the activated medium;

[0047] Activation conditions: inoculate Trichoderma pseudokoningii SMF2 (Trichoderma pseudokoningii SMF2) strain by streaking, activate at 28-30°C for 30 hours, and mix with water to obtain a bacterial suspension.

[0048] (2) The preparation method and culture conditions of the liquid medium are as follows:

[0049] Preparation method of liquid culture medium:

[0050] Mix the treated potato pieces with 4 parts by weight of water p...

Embodiment 3

[0056] The application of the above-mentioned Trichoderma pseudokoningii SMF2 (Trichoderma pseudokoningii SMF2) bacterial strain in the preparation of soil microbial bacterial agent Trichoderma preparation, the steps are as follows:

[0057] (1) The strain preservation number is: CCTCC NO: M209031 Trichoderma pseudokoningii SMF2 (Trichodermapseudokoningii SMF2) is activated by the activation medium, inoculated into a liquid medium for cultivation, and then inoculated into a liquid fermenter medium for fermentation to produce Obtain liquid bacterial classification; This step can refer to embodiment 2;

[0058] (2) Inoculate the liquid strains prepared in step (1) into solid fermentation medium, ferment for 4 days at 25~28° C., relative air humidity of 80~85%, and turn the material once every 24 hours during the fermentation period. Layer ventilation and solid-state fermentation, the thickness of the fermentation material layer is 50cm, and the Trichoderma conidia fermentation c...

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Abstract

The invention relates to a trichoderma pseudokoningii (SMF2) strain and application thereof, which belong to the technical field of microbiology. The invention discloses the trichoderma pseudokoningii (SMF2) strain preserved in China Center for Type Culture Collection on February 24, 2009 with the preservation number being CCTCCNO: M209031, and the application of the strain to preparation of soil microbial agents of trichoderma preparations. The trichoderma pseudokoningii (SMF2) strain disclosed by the invention can be used for producing plant disease fungus cell wall digestive enzymes (CWDEs) and antibiotics of peptaibols at high yield, the CWDEs and the peptaibols cooperate in situ, and the inhibition effect of the trichoderma pseudokoningii strain to the plant disease fungus is further enhanced.

Description

technical field [0001] The invention relates to a strain of Trichoderma pseudokoningii SMF2 (Trichoderma pseudokoningii SMF2) strain and application thereof, belonging to the technical field of microorganisms. Background technique [0002] Plant diseases and insect pests have always been an important factor that seriously affects crop yields, and plant fungal diseases account for more than 90% of plant diseases, such as Verticillium wilt, Fusarium wilt, root rot and other soil-borne diseases of various crops, which are caused by protected cultivation. Downy mildew, gray mold and other leaf diseases in China have increased the incidence area year by year, and the losses caused have increased year by year. At present, there are few effective measures to prevent and control fungal diseases. In order to prevent and control these diseases, farmers have to use a large amount of highly toxic chemical pesticides, resulting in a large amount of pesticide residues in agricultural prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C09K17/00C05F11/08C12R1/885
Inventor 张玉忠石梅宋晓妍陈秀兰孙彩云周百成
Owner SHANDONG UNIV
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