Complex bacteria inoculant for preparing organic fertilizer from effluents of animal slaughter houses and breeding plants serving as raw materials and preparation method thereof

A technology for slaughterhouses and inoculants, applied in the field of agricultural microbe applications, can solve the problems of not being able to meet the production requirements and the difficulty of achieving ideal results, and achieve the effects of promoting growth, improving plant disease resistance, and improving soil

Inactive Publication Date: 2011-02-02
许民强
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the development of bacterial fertilizers that are suitable for specific soil conditions, specific fermentation raw materials, and have specific effects on specialized crops is far from meeting the production requirements.
[0005] There are also many

Method used

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  • Complex bacteria inoculant for preparing organic fertilizer from effluents of animal slaughter houses and breeding plants serving as raw materials and preparation method thereof
  • Complex bacteria inoculant for preparing organic fertilizer from effluents of animal slaughter houses and breeding plants serving as raw materials and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Embodiment 1: the preparation of two bacterium composite inoculants:

[0082] The formulations of the two bacteria are as follows:

[0083] A lactobacillus fermentum 50%

[0084] Bacillus laterosporus 50%

[0085] 1. Preparation of Lactobacillus fermentum fermentation broth A

[0086] Separated and stored, the seed medium is 1% glucose, 1% peptone, and 0.5% beef extract;

[0087] Production adopts deep static fermentation in fermenter;

[0088] The general culture medium for lactic acid bacteria using the prior art: 3% soybean peptone, 2% yeast extract powder, 0.5% sodium acetate, compound trace element solution, compound vitamin solution;

[0089] The fermentation temperature is 40±2°C, and the fermentation time is 48 hours;

[0090] The obtained fermentation broth cfu≥2×10 8 pieces / ml.

[0091] 2. Preparation of single-bacteria fermentation broth B of Bacillus lateralis

[0092] The medium formula is: 3.0% sucrose, 0.8% yeast extract, 1.2% peptone, MgSO 4 0.0...

Embodiment 2

[0101] Embodiment 2: by live bacteria (comprising live spore) quantity percentage content ratio is as follows in the final product:

[0102] A. Lactobacillus fermentum 20%

[0103] B. Bacillus laterosporus 20%

[0104] C, Corning Trichoderma T.koningii 20%

[0105] D. Bacillus azotofixans 20%

[0106] E. Bacillus mucilajginosus 20%

[0107] Completely follow the steps and methods in the implementation method, first obtain the bacterium liquid fermented separately by 5 strains, according to the content of live bacteria (including spores) actually measured in the fermentation liquid of each single strain, live bacteria according to the requirements of the aforementioned formula (spore) ratio, convert the mixed volume ratio of five kinds of bacterial strain fermentation broth, then mix and process into mixed liquid inoculant, concentrate, absorb, dry according to the technology in the embodiment, process into solid inoculant, in the final product The total bacterial content C...

Embodiment 3

[0110] Embodiment 3: by live bacterium (comprising living spore) quantity percentage content ratio is as follows in the final product:

[0111] A. Lactobacillus fermentum 35%

[0112] B. Bacillus laterosporus 35%

[0113] C. Trichoderma koningi T.koningii 10%

[0114] D. Bacillus azotofixans 10%

[0115] E. Bacillus mucilajginosus 10%

[0116] Completely follow the steps and methods in the implementation method to obtain 5 kinds of liquid inoculants respectively.

[0117] The above-mentioned inoculants were circulated and concentrated by using a 50,000-molecular-weight vacuum fiber membrane with a diameter of 18 cm and a length of 110 cm. Concentrate to 20% of original volume.

[0118] The high-temperature sterilized montmorillonite and rice husk powder are mixed and stirred at a ratio of 50% by weight each, and crushed to 80 meshes to form a carrier for dilution and adsorption.

[0119] The above-mentioned five kinds of concentrated liquid inoculants are respectively us...

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Abstract

The invention provides a complex bacteria inoculant for preparing an organic fertilizer from effluents of animal slaughter houses and breeding plants serving as raw materials, which has the advantages of effectively deodorizing and enhancing the fertility, and a preparation method thereof. The complex bacteria liquid inoculant is prepared by the following steps of: independently performing liquidfermentation on each single bacterium, namely A, lactobacillus fermentium, B, bacillus laterosporus, C, trichoderma koningii, D, nitrogen-fixing bacteria spores and E, bacillus mucilaginosus; and mixing the bacteria, wherein the final product comprises the living bacteria (including living spores) in percentage by weight: 20 to 50 percent of A, 20 to 50 percent of B, 0 to 20 percent of C, 0 to 20percent of D and 0 to 20 percent E. The liquid inoculant is further processed so as to obtain solid inoculant. The complex inoculant of the invention is specially used for preparing the organic fertilizer from excrements of pig farms (or chicken farms) and wastes of slaughter houses thereof, which serve as basic raw materials, and a small amount of straws, and has an obvious effect of reducing odor of the fertilizer and the field. The complex inoculant combines the functions of improving the disease resistance of plants, promoting growth and improving soil and the fertility of the fertilizer.

Description

technical field [0001] The invention belongs to the application field of agricultural microorganisms, in particular to the professional scope of microbial organic fertilizers. technical background [0002] The extensive use of chemical fertilizers in the agricultural field has seriously damaged human life and the ecological environment. Due to the residue of drugs and the deterioration of soil structure, agriculture has fallen into a serious ecological crisis. Modern biotechnology and biological control technology, with agricultural microbial technology as an important aspect, are effective and important measures to deal with the above crisis, and are also one of the important trends of modern agriculture. [0003] Over the past half a century, especially in the past two decades, in the field of biotechnology, the application of nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-solubilizing bacteria, and photosynthetic bacteria in agriculture has also ma...

Claims

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Application Information

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IPC IPC(8): C12R1/225C12R1/885C12R1/07C12N1/14C05F3/00C05F11/08C12N1/20
CPCY02A40/20
Inventor 许民强
Owner 许民强
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