Method of inhibiting penicillium expansum and application of method

A technology to expand Penicillium and light intensity, applied in the direction of microorganism-based methods, biochemical equipment and methods, and treatment of microorganisms with electricity/wave energy, which can solve problems such as unsuitability for industrial applications, high processing costs, and radiation pollution. Achieve the effects of no radiation pollution risk, simple operation, and no secondary pollution

Inactive Publication Date: 2018-05-22
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The current physical prevention and control technologies such as γ-rays, pulsed strong light, pulsed magnetic field, infrared radiation, microwave radiation, and ultraviolet

Method used

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  • Method of inhibiting penicillium expansum and application of method
  • Method of inhibiting penicillium expansum and application of method
  • Method of inhibiting penicillium expansum and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The first step: Prepare PDA solid medium: glucose 20g, agar 15g, water 1000ml, weigh 200g of peeled potatoes and fresh potatoes according to the medium formula, cut the potatoes into small pieces and put them in the pot, add 1000ml of water, and heat on the heater Bring to a boil, keep it for 20-30 minutes, filter it on the measuring cup with 2 layers of gauze while it is hot, discard the filter residue, add water to the filtrate to 1000ml, heat to dissolve, put the filtrate into the pot, add 20g of glucose, 150g of agar, heat on low heat , and use a glass rod to stir continuously to prevent the agar from sticking to the bottom or overflowing. After the agar is completely dissolved, add water to the required amount. While hot, put 25ml of the prepared medium into a flat petri dish, and heat it at 121℃ Autoclaved, ready to use;

[0049] The second step: take out the Cerium sp. strains stored in the refrigerator at 4°C and inoculate them on the PDA solid medium;

[0050]...

Embodiment 2

[0052] Carry out the first step and the second step according to the method for embodiment 1, the difference is that the third step is implemented in this way: the PDA solid culture medium inoculated with the expanded Cerium strain is placed in an intelligent incubator to cultivate, and the blue light of the blue LED light Adjust the light intensity to 750lx, and irradiate at 37°C for 60 hours.

Embodiment 3

[0054] Carry out the first step and the second step according to the method for embodiment 1, the difference is that the third step is implemented in this way: the PDA solid culture medium inoculated with the expanded Cerium strain is placed in an intelligent incubator to cultivate, and the blue light of the blue LED light Adjust the light intensity to 1000lx, and irradiate at 20°C for 40 hours.

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Abstract

The invention discloses a method of inhibiting penicillium expansum and application of the method. The method comprises the step of illuminating the penicillium expansum with light in the growth process of the penicillium expansum, wherein the light is one or more of blue light, green light, red light and yellow light. According to the method provided by the invention, in the growth process of thepenicillium expansum, one or more of the blue light, the green light, the red light and the yellow light are applied to illuminating the penicillium expansum, and the inhibition effect to the penicillium expansum can be realized. The method does not have the radiation pollution risk, is low in cost and simple to operate, does not cause secondary pollution, has relatively high popularization and application value and is particularly suitable for inhibiting growth of the penicillium expansum at places (such as food preservation) needing to inhibit the penicillium expansum.

Description

technical field [0001] The invention relates to a method for inhibiting Penicillium, in particular to a method for inhibiting Penicillium extensa and its application. Background technique [0002] Phyllostachys extensa is the main fungus responsible for the occurrence of Phyllostachys extremosa, and it is easy to parasitize on fruits and vegetables. Phyllostachys extensa can produce a variety of mycotoxins, such as patulin, also known as patulin, patulin, and coral penicillin , can cause gastrointestinal dysfunction in animals and edema and hemorrhage in different organs, leading to symptoms such as diarrhea and poisoning; high doses can even cause cancer and teratogenicity. According to the statistics of the Food and Agriculture Organization of the United Nations, 25% of the food in the world is contaminated with mycotoxins every year, and the resulting economic losses amount to hundreds of billions of dollars. Therefore, it is particularly important to find a degradation ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N13/00C12R1/83
CPCC12N1/14C12N13/00
Inventor 蒋红梅方俊程翠利刘晓颖苏招红田云
Owner HUNAN AGRICULTURAL UNIV
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