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Microbial composite bacterial preparation and application thereof to preparation of fermented feed

A technology of microbial compound bacteria and compound bacteria agent, applied in the direction of microorganism-based methods, chemicals for biological control, microorganisms, etc., can solve the problems of food safety, crop spoilage, feed spoilage, etc. Nutrient-rich, non-perishable safety effects

Active Publication Date: 2020-08-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to being rich in nutrients such as amino acids and moisture, crop wastes are easily attacked by filamentous fungi, while filamentous fungi such as Penicillium expanses, A. The leaves, fruits, seeds, etc. of the crops cause corruption of the crops, which in turn causes storage diseases of the crops, and they produce toxins such as patulin and citrinin, which will remain in the crops and then enter the human body through the food chain, which is potentially harmful nature) are one of the culprits causing crop spoilage, therefore, even after sterilization, the feed prepared from crops is also easy to spoil during storage
[0005] In order to solve this problem, people such as Ma Qingsong tried to add preservatives to the feed prepared from crops to prolong its shelf life (see references for details: Ma Qingsong, Wang Weiguo. The impact of different antifungal agents on the storage quality of pellet feed [J]. Feed Industry, 2019, 40(9): 38-44), however, there are food safety problems in adding a large amount of preservatives, and chemical additives have many restrictions such as high cost, low return and unfavorable environmental protection (specifically Visible references: Yu Zhengrong, Wu Yinchao, Xie Jiayu, etc. Antifungal agent sodium dehydroacetate induced bleeding test in rats [J]. Advances in Veterinary Medicine, 2018,39(1):73-78 and references: Wang Xueyang, Han Shumin, Li Jinku, etc. Application of antifungal agents in feed [J]. Animal Husbandry and Feed Science, 2019, 40(3):50-52), and insufficient preservative addition will lead to poor antiseptic effect

Method used

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  • Microbial composite bacterial preparation and application thereof to preparation of fermented feed
  • Microbial composite bacterial preparation and application thereof to preparation of fermented feed
  • Microbial composite bacterial preparation and application thereof to preparation of fermented feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0066] Embodiment 1-1: Screening, identification, cultivation and observation of Bifidobacterium longum (Bifidobacterium longum)

[0067] 1. Screening

[0068] Take 1g of feces samples from healthy adults in Wuxi area, dilute them with normal saline, spread them on mMRS solid medium, culture them in an anaerobic environment at 37°C for 72 hours, observe and record the colony morphology; pick colonies in mMRS Streak on the solid medium, carry out purification culture at 37°C in an anaerobic environment, and obtain a purified single colony; pick a single colony and streak on the mMRS solid medium, culture anaerobically at 37°C for 48 hours, and test the obtained colony Gram staining (Gram staining method refers to the textbook "Industrial Microbial Breeding" author: Zhuge Jian), record the colony morphology, and investigate the physiological and biochemical characteristics of the strain according to the textbook "Common Bacterial System Identification Manual" (Author: Dong Xiuzh...

Embodiment 1-2

[0083] Example 1-2: Effect of Bifidobacterium longum CCFM1109 and its fermentation supernatant on the germination rate of filamentous fungal spores

[0084] 1. Effect of Bifidobacterium longum (Bifidobacterium longum) CCFM1109 on the germination rate of filamentous fungal spores (double-layer plate-growth inhibition method)

[0085] Pick the single bacterium colony of Bifidobacterium longum (Bifidobacterium longum) CCFM1109 screened in Example 1 and streak it on the mMRS solid medium, cultivate it at 37° C. for 48 hours in an anaerobic environment to obtain a single bacterium colony; pick a single bacterium colony and inoculate it in the mMRS liquid In the culture medium, culture was carried out at 37°C for 48 hours under anaerobic environment, and this operation was repeated 3 times to obtain the bacterial liquid cultivated to the third generation.

[0086] Use an inoculation loop to inoculate the Penicillium extensa liquid in the ampoule tube on the PDA medium, and incubate ...

Embodiment 1-3

[0102] Example 1-3: Effect of Bifidobacterium longum (Bifidobacterium longum) CCFM1109 fermentation supernatant on the growth of filamentous fungal mycelium

[0103] Mix the mMRS liquid medium with the PDA medium at a volume ratio of 1:9, 1.5:8.5, 2:8, 2.5:7.5, 3:7 (mMRS liquid medium: PDA medium) to obtain mMRS liquid medium Concentration is respectively the control group mixed solution of 10,15,20,25,30% (v / v); The fermentation supernatant that embodiment 2 obtains is respectively with 1:9,1.5:8.5,2:8,2.5: The volume ratio of 7.5, 3:7 (fermentation supernatant: PDA medium) was mixed with PDA medium to obtain experimental groups with fermentation supernatant concentrations of 10, 15, 20, 25, 30% (v / v) respectively Mixed solution; Pour the mixed solution of the control group and the experimental group into the plate respectively; add 10 μL of the Penicillium expanse spore suspension obtained in Example 2 dropwise to the center of the plate, and cultivate it at 28°C for 6 days....

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Abstract

The invention relates to a microbial composite bacterial preparation and application thereof to preparation of a fermented feed, and belongs to the technical field of biology and the technical field of fermentation. The invention provides a microbial composite bacterial preparation which is high in safety and can effectively inhibit filamentous fungi such as penicillium expansum, aspergillus niger, penicillium roqueforti, penicillium digitatum and the like. The microbial composite bacterial preparation consists of bifidobacterium longum with the preservation number of GDMCC No.60926 and lactobacillus plantarum with the preservation number of CGMCC No.5494; the fermented feed prepared by utilizing the microbial composite bacterial preparation is rich in nutrient, not liable to decay and very high in safety; and the concrete embodiment is that: in the fermented feed prepared by utilizing the microbial composite bacterial preparation, content of mould is 0 CFU / g, and after the fermented feed is placed for 15d at a temperature of 30 DEG C, the content of the mould in the fermented feed prepared by utilizing the microbial composite bacterial preparation is still 0 CFU / g.

Description

technical field [0001] The invention relates to a microbial compound bacterial agent and its application in preparing fermented feed, and belongs to the field of biotechnology and fermentation technology. Background technique [0002] my country is a large agricultural country, but in the process of crop processing, it is inevitable that a large amount of waste will be generated, for example, apple pomace will be produced in the production of concentrated apple juice, blueberry pomace will be produced in the production of blueberry juice, and mulberry fruit will be produced in the production of mulberry juice. Fruit pomace, the production of grape juice will produce fruit waste such as grape pomace, the production of hawthorn extract will produce hawthorn pomace, the production of starch will produce cassava pomace, the production of dried carrots will produce vegetable waste such as carrot pomace, and the production of soybean oil will produce The production of soybean meal a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/12A01N63/20A01P3/00C12R1/01C12R1/25
CPCA23K10/12A01N63/20C12N1/20C12N1/205C12R2001/01
Inventor 于雷雷陈卫乔楠桢翟齐啸田丰伟赵建新张灏
Owner JIANGNAN UNIV
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