Microbial composite bacterial preparation and application thereof to preparation of fermented feed
A technology of microbial compound bacteria and compound bacteria agent, applied in the direction of microorganism-based methods, chemicals for biological control, microorganisms, etc., can solve the problems of food safety, crop spoilage, feed spoilage, etc. Nutrient-rich, non-perishable safety effects
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Embodiment 1-1
[0066] Embodiment 1-1: Screening, identification, cultivation and observation of Bifidobacterium longum (Bifidobacterium longum)
[0067] 1. Screening
[0068] Take 1g of feces samples from healthy adults in Wuxi area, dilute them with normal saline, spread them on mMRS solid medium, culture them in an anaerobic environment at 37°C for 72 hours, observe and record the colony morphology; pick colonies in mMRS Streak on the solid medium, carry out purification culture at 37°C in an anaerobic environment, and obtain a purified single colony; pick a single colony and streak on the mMRS solid medium, culture anaerobically at 37°C for 48 hours, and test the obtained colony Gram staining (Gram staining method refers to the textbook "Industrial Microbial Breeding" author: Zhuge Jian), record the colony morphology, and investigate the physiological and biochemical characteristics of the strain according to the textbook "Common Bacterial System Identification Manual" (Author: Dong Xiuzh...
Embodiment 1-2
[0083] Example 1-2: Effect of Bifidobacterium longum CCFM1109 and its fermentation supernatant on the germination rate of filamentous fungal spores
[0084] 1. Effect of Bifidobacterium longum (Bifidobacterium longum) CCFM1109 on the germination rate of filamentous fungal spores (double-layer plate-growth inhibition method)
[0085] Pick the single bacterium colony of Bifidobacterium longum (Bifidobacterium longum) CCFM1109 screened in Example 1 and streak it on the mMRS solid medium, cultivate it at 37° C. for 48 hours in an anaerobic environment to obtain a single bacterium colony; pick a single bacterium colony and inoculate it in the mMRS liquid In the culture medium, culture was carried out at 37°C for 48 hours under anaerobic environment, and this operation was repeated 3 times to obtain the bacterial liquid cultivated to the third generation.
[0086] Use an inoculation loop to inoculate the Penicillium extensa liquid in the ampoule tube on the PDA medium, and incubate ...
Embodiment 1-3
[0102] Example 1-3: Effect of Bifidobacterium longum (Bifidobacterium longum) CCFM1109 fermentation supernatant on the growth of filamentous fungal mycelium
[0103] Mix the mMRS liquid medium with the PDA medium at a volume ratio of 1:9, 1.5:8.5, 2:8, 2.5:7.5, 3:7 (mMRS liquid medium: PDA medium) to obtain mMRS liquid medium Concentration is respectively the control group mixed solution of 10,15,20,25,30% (v / v); The fermentation supernatant that embodiment 2 obtains is respectively with 1:9,1.5:8.5,2:8,2.5: The volume ratio of 7.5, 3:7 (fermentation supernatant: PDA medium) was mixed with PDA medium to obtain experimental groups with fermentation supernatant concentrations of 10, 15, 20, 25, 30% (v / v) respectively Mixed solution; Pour the mixed solution of the control group and the experimental group into the plate respectively; add 10 μL of the Penicillium expanse spore suspension obtained in Example 2 dropwise to the center of the plate, and cultivate it at 28°C for 6 days....
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