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Preparation method of biodiesel from microalgal oil by enzyme method

A technology for microalgae oil and biodiesel, which is applied in the production of oil/fat, biofuel, and fat production, can solve the problems of high price and unfavorable industrial production of biodiesel, and achieve the effects of low price, reduced investment and easy separation.

Inactive Publication Date: 2011-09-21
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the lipase used for biodiesel research at home and abroad is mainly Novozym 435 produced by Novozymes. This enzyme is a resin-immobilized enzyme, and the carrier is a macroporous acrylic resin. The price is high, which is not conducive to the industrial production of biodiesel prepared by enzymatic method.

Method used

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  • Preparation method of biodiesel from microalgal oil by enzyme method
  • Preparation method of biodiesel from microalgal oil by enzyme method
  • Preparation method of biodiesel from microalgal oil by enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of biodiesel from microalgae oil catalyzed by PEL in ionic liquid [BMIm][PF6]:

[0030] The reaction conditions in the ionic liquid [BMIm][PF6] system are: alcohol to oil molar ratio 3: 1, enzyme amount 100mg, solvent 2mL / g (based on oil weight, v / w), water content 3% (based on oil weight, w / w), the reaction temperature is 45°C. The reaction solution was taken regularly for methyl ester content analysis. Take 50 μL of the reaction solution and centrifuge to separate layers, take 10 μL of the upper liquid sample, dissolve it in 290 μL of n-hexane, mix well, then add 300 μL of methyl heptadecenenoate (2 mg / mL) as an internal standard; The amount of fatty acid methyl ester in the reactant was determined by chromatography.

[0031] Under the above conditions, the conversion rate of biodiesel can be as high as 91% after 48 hours of reaction.

Embodiment 2

[0033] Preparation of biodiesel from microalgae oil catalyzed by PEL in tert-butanol:

[0034] The reaction conditions in the tert-butanol system are: alcohol oil molar ratio 3: 1, enzyme amount 100mg, solvent 1mL / g (based on oil weight, v / w), water content 3% (based on oil weight, w / w), The reaction temperature is 45°C. The reaction solution was taken regularly for methyl ester content analysis. Take 50 μL of the reaction solution and centrifuge to separate layers, take 10 μL of the upper liquid sample, dissolve it in 290 μL of n-hexane, mix well, then add 300 μL of methyl heptadecenenoate (2 mg / mL) as an internal standard; The amount of fatty acid methyl ester in the reactant was determined by chromatography.

[0035] Under the above conditions, the conversion rate of biodiesel can reach 49% after 48 hours of reaction.

[0036] from figure 1 with It can also be seen from Figure 2 that in the ionic liquid [BMIm][PF6], PEL catalyzes the preparation of biodiesel from mic...

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Abstract

Belonging to the biochemical field, the invention provides a preparation method of biodiesel from microalgal oil by enzyme method in a novel reaction medium. The method comprises the steps of: (1) providing algae powder made from dried microalgaes; (2) extracting microalgal oil from the algae powder; (3) characterizing the microalgal oil with physiochemical constants, and acquiring a saponification value so as to determine the amount of methanol needed; and (4) with ionic liquid (BMIm)(PF6) or tert-butyl alcohol as the reaction medium, adding methanol which contains alcohol and oil in a ratio ranging from 1:2 to 5:1 by mole into the microalgal oil, and adding 50-400mg penicillium expansum lipase for reaction, thus obtaining fatty acid methyl ester as the product.

Description

【Technical field】 [0001] The invention belongs to the field of biochemical industry, in particular to a method for preparing microalgae oil biodiesel by enzymatic method. 【Background technique】 [0002] Facing the environmental problems caused by the depletion of resources and the increase in the proportion of atmospheric carbon dioxide, people began to realize that renewable biofuels are crucial to the sustainable development of the environment and economy. Biodiesel refers to the alkyl ester compound produced by esterification and transesterification of oil and short-chain alcohol under the action of catalyst. Biodiesel is a typical "green energy". Vigorously developing biodiesel has important strategic significance for sustainable economic development, promoting energy substitution, reducing environmental pressure, and controlling urban air pollution. [0003] Matti Lievonen, CEO of Nestor Petroleum, the world's leading biodiesel producer, recently stated that by 2020, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/04C11B1/10C11C3/04C12P7/64C10L1/02
CPCY02E50/13Y02E50/10
Inventor 杨缜胡章立赖璟琦
Owner SHENZHEN UNIV
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