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Culture medium for producing griseofulvin through fermentation of penicillium expansum and culture method

A fermentation medium and culture method technology, applied in the field of medium for producing griseofulvin, can solve the problems of high energy consumption, long fermentation period of kasugamycin, inability to provide effective technical support for large-scale production mode, and achieve fermentation The effect of unit improvement and reduction of fermentation energy consumption

Inactive Publication Date: 2017-03-08
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 2 There is a lack of literature reports on the complete fermentation process of large-scale production of kasugamycin in China, and the relevant literature reports are mainly experiments, which cannot provide effective technical support for large-scale production models
[0006] 3 The large-scale production of kasugamycin has a long fermentation period, exceeding about 260 hours, and the energy consumption is relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Seed culture: seed medium volume 10m 3 .

[0059] Pueraria root powder 70kg, Hercules sweet sorghum 120kg, glucose 50kg, distiller's grains 60kg, corn steep liquor 110L, fish meal 100kg, cottonseed oil 80L, ammonium sulfate 4kg, light calcium carbonate 10kg.

[0060] First, sterilize the seed medium and cool it to 20°C, and keep the pressure with sterile air. The quality of the medium after sterilization is: amino nitrogen 20mg / L, reducing sugar 2g / 100ml. Then, under flame protection, the fermented liquid of the patulapenicillium mother bottle that has been cultivated is inserted into the seed medium according to the inoculum size of 30 L to carry out seed culture.

[0061] Seed culture conditions are: tank pressure 0.03-0.06MPa; tank temperature 28-29°C; air flow: 30m 3 / h; stirring speed 60r / min. Inoculate until the cell concentration is 32%, and the culture time is 40h.

[0062] Fermentation culture: fermentation medium volume 100m 3 .

[0063] Pueraria root powd...

Embodiment 2

[0077] Seed culture: Seed medium volume 10m 3 .

[0078] Pueraria root powder 80kg, Hercules sweet sorghum 130kg, glucose 60kg, white distiller's grains 70kg, corn steep liquor 120L, fish meal 110kg, cottonseed oil 90L, ammonium sulfate 5kg, light calcium carbonate 20kg.

[0079] First, the seed medium was sterilized and cooled to 21°C, and the pressure was maintained with sterile air. The mass of the medium after sterilization was: amino nitrogen 25mg / L, reducing sugar 2.5g / 100ml. Then, under the protection of flame, the cultured Penicillium patulatum mother bottle fermentation broth was inserted into the seed medium according to the inoculum amount of 32L to carry out seed culture.

[0080] Seed cultivation conditions are: tank pressure 0.03-0.06MPa; tank temperature 28-29°C; air flow: 35m 3 / h; stirring speed 65r / min. The cells were transplanted to a concentration of 32.5% and a culture time of 41h.

[0081] Fermentation culture: fermentation medium volume 100m 3 .

...

Embodiment 3

[0097] Seed culture: Seed medium volume 10m 3 .

[0098] Pueraria root powder 90kg, Hercules sweet sorghum 140kg, glucose 70kg, white distiller's grains 80kg, corn steep liquor 130L, fish meal 120kg, cottonseed oil 100L, ammonium sulfate 6kg, light calcium carbonate 30kg.

[0099] First, the seed culture medium was sterilized and cooled to 22°C, and the pressure was maintained with sterile air. The quality of the culture medium after sterilization was: amino nitrogen 30 mg / L, reducing sugar 3 g / 100 ml. Then, under the protection of flame, the cultured Penicillium patulatum mother bottle fermentation broth was inserted into the seed medium according to the inoculum amount of 35L to carry out seed culture.

[0100] Seed cultivation conditions are: tank pressure 0.03-0.06MPa; tank temperature 28-29°C; air flow: 40m 3 / h; stirring speed 70r / min. The cells were transplanted to a concentration of 33% and a culture time of 43h.

[0101] Fermentation culture: fermentation medium v...

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Abstract

The invention relates to a culture medium for producing griseofulvin through fermentation of penicillium expansum and a culture method. A seed culture medium for producing griseofulvin through fermentation of penicillium expansum, a carbon source and a nitrogen source in a fermentation culture medium and the best compatibility proportion are confirmed. With the adoption of the formula of the culture medium and the fermentation control process, the fermentation unit of griseofulvin can be increased, the fermentation cost can be reduced, raw materials and auxiliary materials are reduced to the largest extent, are free of environmental influence and can be sufficiently supplied, and stable and efficient production of griseofulvin is realized.

Description

technical field [0001] The invention belongs to the field of fermentation technology, in particular to a culture medium and a culture method for producing griseofulvin by fermentation of patulin. Background technique [0002] Griseofulvin is a chlorine-containing secondary metabolite isolated from the mycelium of Penicillium fermentation broth. It is an antifungal antibiotic belonging to the non-polyene class. Griseofulvin began to be used in clinical medicine in 1958, mainly for the treatment of superficial filamentous fungal infections of the skin, especially for Microsporum and rubrum. In agriculture, griseofulvin is also used for fungal disease control. [0003] At present, domestic production of griseofulvin by fermentation mode has the following main problems: [0004] 1 The fermentation level of industrialized large-scale production is low, and its fermentation level is maintained at about 25000ug / ml. [0005] 2 There is a lack of literature reports on the complet...

Claims

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Application Information

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IPC IPC(8): C12P17/04C12N1/14C12R1/83
CPCC12P17/04C12N1/14
Inventor 任勇
Owner 宁夏泰瑞制药股份有限公司
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