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Bifidobacterium adolescentis capable of inhibiting filamentous fungi and application of bifidobacterium adolescentis

A technology of filamentous fungi and bifidobacteria, applied in the field of bifidobacteria adolescentis, can solve the problems of short residence time, poor effect of biological control methods, and non-sustainable physical control methods, and achieve good acid stability and high The effect of applying the foreground

Active Publication Date: 2020-08-14
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the physical control method is mainly to inhibit the infection of filamentous fungi by microwave heating and cold storage of fruits and vegetables. However, due to the short time between the physical means such as microwave heating and cold storage on the surface of fruits and vegetables, the physical control method is not sustainable.
[0006] The biological control method mainly inhibits filamentous fungal infection by antagonizing microorganisms. Compared with physical control methods, this method is more sustainable. However, the biological control method has the disadvantage of poor effect, mainly because many antagonistic microorganisms themselves belong to fungi. , can also cause fungal spoilage on the fruit surface

Method used

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  • Bifidobacterium adolescentis capable of inhibiting filamentous fungi and application of bifidobacterium adolescentis
  • Bifidobacterium adolescentis capable of inhibiting filamentous fungi and application of bifidobacterium adolescentis
  • Bifidobacterium adolescentis capable of inhibiting filamentous fungi and application of bifidobacterium adolescentis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Screening, identification, cultivation and observation of Bifidobacterium adolescentic CCFM1108

[0049] 1. Screening

[0050] Take 1g of feces samples from healthy adults in Wuxi area, dilute them with normal saline, spread them on mMRS solid medium, culture them in an anaerobic environment at 37°C for 72 hours, observe and record the colony morphology; pick colonies in mMRS Streak on the solid medium, carry out purification culture at 37°C in an anaerobic environment, and obtain a purified single colony; pick a single colony and streak on the mMRS solid medium, culture anaerobically at 37°C for 48 hours, and test the obtained colony Gram staining (Gram staining method refers to the textbook "Industrial Microbial Breeding" author: Zhuge Jian), record the colony morphology, and investigate the physiological and biochemical characteristics of the strain according to the textbook "Common Bacterial System Identification Manual" (Author: Dong Xiuzhu) , to retain...

Embodiment 2

[0065] Example 2: Effect of Bifidobacterium adolescentic CCFM1108 and its fermentation supernatant on the germination rate of filamentous fungal spores

[0066] 1. The effect of Bifidobacterium adolescentic CCFM1108 on the germination rate of filamentous fungal spores (double-layer plate-growth inhibition method)

[0067] Pick the single colony of Bifidobacterium adolescentic (Bifidobacterium adolescentic) CCFM1108 screened in Example 1 and streak it on the mMRS solid medium, and culture it at 37°C for 48h under anaerobic environment to obtain a single colony; pick a single colony and inoculate it in the mMRS liquid In the culture medium, cultivate at 37° C. for 48 hours under anaerobic environment, repeat this operation 3 times, and obtain the bacterial liquid cultivated to the third generation.

[0068]Use an inoculation loop to inoculate the Penicillium extensa liquid in the ampoule tube on the PDA medium, and incubate at 28°C and 200rpm for 7 days to obtain mycelium and sp...

Embodiment 3

[0083] Embodiment 3: the influence of bifidobacterium adolescentic (Bifidobacterium adolescentic) CCFM1108 fermentation supernatant on the growth of Penicillium expanses mycelium

[0084] Mix the mMRS liquid medium with the PDA medium at a volume ratio of 1:9, 1.5:8.5, 2:8, 2.5:7.5, 3:7 (mMRS liquid medium: PDA medium) to obtain mMRS liquid medium Concentration is respectively the control group mixed solution of 10,15,20,25,30% (v / v); The fermentation supernatant that embodiment 2 obtains is respectively 1:9,1.5:8.5,2:8,2.5: The volume ratio of 7.5, 3:7 (fermentation supernatant: PDA medium) was mixed with PDA medium to obtain experimental groups with fermentation supernatant concentrations of 10, 15, 20, 25, 30% (v / v) respectively Mixed solution; Pour the mixed solution of the control group and the experimental group into the plate respectively; add 10 μL of the Penicillium expanse spore suspension obtained in Example 2 dropwise to the center of the plate, and cultivate it at...

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Abstract

The invention discloses bifidobacterium adolescentis capable of inhibiting filamentous fungi and an application of the bifidobacterium adolescentis, and belongs to the technical field of biology. Theinvention provides a bifidobacterium adolescentis CCFM1108 strain, and the strain can inhibit filamentous fungi. Inhibition of filamentous fungi is specifically reflected in that after the strain is co-cultured with penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores, the germination rate of the penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores can be remarkably inhibited within 7 days; and after the fermentation supernatant of the strain is separately co-culturedwith penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores, germination of the penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores can be remarkably inhibited, and the spore germination inhibition rates can reach 99.88+ / -0.66%, 92.23+ / -0.53%, 88.83+ / -0.82% and 99.25+ / -0.62% respectively.

Description

technical field [0001] The invention relates to a bifidobacterium adolescent capable of inhibiting filamentous fungi and its application, belonging to the field of biotechnology. Background technique [0002] Fruit and vegetable spoilage can lead to cosmetic defects and nutritional deficiencies in fruits and vegetables. In developing countries with inadequate refrigeration and transportation facilities, the problem of fruit and vegetable spoilage is particularly prominent, and the losses it causes to the fruit and vegetable industry are also particularly serious. [0003] Penicillium extensa, Aspergillus niger, Penicillium loudii, Penicillium digitatum and other filamentous fungi are one of the culprits that cause fruit and vegetable spoilage. They mainly cause fruit and vegetable spoilage by eroding the leaves and fruits of fruits and vegetables, and then cause storage diseases of fruits and vegetables. , and they will produce toxins (for example, patulin and citrinin), th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/01
CPCC12N1/20A23B7/155C12R2001/01C12N1/205A23V2400/513
Inventor 田丰伟陈卫翟齐啸乔楠桢于雷雷闫博文赵建新张灏
Owner JIANGNAN UNIV
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