A kind of preparation method of open arrow extract and application thereof
An open arrow and extract technology, which is applied in the field of preparation of open arrow extract, can solve the problems of poor antibacterial effect of the extract, high solvent toxicity, susceptibility to microorganisms, etc., and achieves wide antibacterial spectrum, high extraction rate and less residue. Effect
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Embodiment 1
[0030] 100g of open arrow rhizome coarse powder of 18-30 mesh, add 1000mL 95% ethanol (the volume fraction of ethanol is 95%), ultrasonic-assisted extraction at room temperature for 20min, after soaking at room temperature for 12h, stir evenly, and then ultrasonically at room temperature for 20min, then Place in a water bath at 50°C for constant temperature extraction for 3 hours, suction filter, and repeat the same method twice; combine the filtrates, concentrate under reduced pressure at 50°C to obtain an extract, freeze-dry to constant weight, and the yield is about 10%.
Embodiment 2
[0032] 200g of open arrow rhizome powder 18-30 mesh, add 2000mL ethyl acetate, ultrasonically assisted extraction at room temperature for 30min, then soak at room temperature for 18h, stir well, then ultrasonically at room temperature for 20min, then place in a water bath at a constant temperature of 55°C Extract for 3 hours, filter with suction, and repeat the same method twice; combine the filtrates, concentrate under reduced pressure at 60°C to an extract, freeze-dry to constant weight, and the yield is about 1.85%.
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