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A kind of preparation method of open arrow extract and application thereof
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An open arrow and extract technology, which is applied in the field of preparation of open arrow extract, can solve the problems of poor antibacterial effect of the extract, high solvent toxicity, susceptibility to microorganisms, etc., and achieves wide antibacterial spectrum, high extraction rate and less residue. Effect
Active Publication Date: 2021-09-24
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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Problems solved by technology
[0003] Although physical fresh-keeping methods such as controlled atmosphere and refrigeration can effectively delay the physiological and biochemical processes of fruits and vegetables after harvest and maintain the freshness of fruits, their disadvantages are that once they leave the original physical environment, especially after mechanical damage, they are easily infected by microorganisms, especially fungi. mildew and rot
Although there are related reports on the use of the open arrow extract for antibacterial in the prior art, there are defects such as complex process, high solvent toxicity, large usage, high cost, large residue, and poor antibacterial effect of the extract.
Method used
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Embodiment 1
[0030] 100g of open arrow rhizome coarse powder of 18-30 mesh, add 1000mL 95% ethanol (the volume fraction of ethanol is 95%), ultrasonic-assisted extraction at room temperature for 20min, after soaking at room temperature for 12h, stir evenly, and then ultrasonically at room temperature for 20min, then Place in a water bath at 50°C for constant temperature extraction for 3 hours, suction filter, and repeat the same method twice; combine the filtrates, concentrate under reduced pressure at 50°C to obtain an extract, freeze-dry to constant weight, and the yield is about 10%.
Embodiment 2
[0032] 200g of open arrow rhizome powder 18-30 mesh, add 2000mL ethyl acetate, ultrasonically assisted extraction at room temperature for 30min, then soak at room temperature for 18h, stir well, then ultrasonically at room temperature for 20min, then place in a water bath at a constant temperature of 55°C Extract for 3 hours, filter with suction, and repeat the same method twice; combine the filtrates, concentrate under reduced pressure at 60°C to an extract, freeze-dry to constant weight, and the yield is about 1.85%.
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Abstract
The invention discloses a preparation method and application of an open arrow extract. The preparation method of the open arrow extract uses open arrow rhizomes as raw materials, extracts them with ethanol aqueous solution or ethyl acetate as a solvent, concentrates, and dries them. To obtain the open arrow extract, when the solvent is ethanol aqueous solution, the volume fraction of ethanol is not less than 60%. The present invention uses ethanol-water solution or ethyl acetate as a solvent to prepare the open arrow extract, all of which are completed in one step, without repeated extraction with multiple solvents, simple process operation, high extraction rate, low cost, and environmentally friendly; the prepared extract It can effectively inhibit the growth of filamentous fungi, especially the inhibitory effect on Penicillium expansica, Penicillium italicum, Penicillium digitatum, Aspergillus niger, Rhizopus stoloniferus, Botrytis cinerea, etc. / mL) has good effect, broad antibacterial spectrum, no harmful organic solvent residue, environment-friendly, less toxic and side effects on non-targets, and conforms to the sustainable development concept of green environmental protection.
Description
technical field [0001] The invention relates to a preparation method and application of an open arrow extract, belonging to the field of open arrow extraction. Background technique [0002] With the improvement of living standards, more and more people begin to pay attention to the safety of preservatives in the storage process of fruits and vegetables and other foods. Tobuzin, etc., as well as sec-butylamine and its derivatives, Xianbao, Baidusha, etc., are mostly chemically synthesized. Overdosage of drugs is common in some areas or crops, posing risks to the quality and safety of agricultural products. [0003] Although physical fresh-keeping methods such as controlled atmosphere and refrigeration can effectively delay the physiological and biochemical processes of fruits and vegetables after harvest and maintain the freshness of fruits, their disadvantages are that once they leave the original physical environment, especially after mechanical damage, they are easily infe...
Claims
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Application Information
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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/896A61P31/10
Inventor 商士斌姚姝凤王丹刘鹤张海波高宏
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY