Bifidobacterium longum and application thereof in inhibiting filamentous fungi

A technology of Bifidobacterium longum and filamentous fungi, applied in the direction of bifidobacteria, applications, fungi, etc., can solve the problems of short stay, poor effect of biological control methods, and non-sustainable physical control methods, achieving The effect of good acid stability and high application prospects

Active Publication Date: 2020-05-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the physical control method is mainly to inhibit the infection of filamentous fungi by microwave heating and cold storage of fruits and vegetables. However, due to the short time between the physical means such as microwave heating and cold storage on the surface of fruits and vegetables, the physical control method is not sustainable.
[0007] The biological control method mainly inhibits filamentous fungal infection by antagonizing microorganisms. Compared with physical control methods, this method is more sustainable. However, the biological control method has the disadvantage of poor effect, mainly because many antagonistic microorganisms themselves belong to fungi. , can also cause fungal spoilage on the fruit surface

Method used

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  • Bifidobacterium longum and application thereof in inhibiting filamentous fungi
  • Bifidobacterium longum and application thereof in inhibiting filamentous fungi
  • Bifidobacterium longum and application thereof in inhibiting filamentous fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: Screening, identification, cultivation and observation of Bifidobacterium longum (Bifidobacterium longum)

[0052] 1. Screening

[0053] Take 1g of feces samples from healthy adults in Wuxi area, dilute them with normal saline, spread them on mMRS solid medium, culture them in an anaerobic environment at 37°C for 72 hours, observe and record the colony morphology; pick colonies in mMRS Streak on the solid medium, carry out purification culture at 37°C in an anaerobic environment, and obtain a purified single colony; pick a single colony and streak on the mMRS solid medium, culture anaerobically at 37°C for 48 hours, and test the obtained colony Gram staining (Gram staining method refers to the textbook "Industrial Microbial Breeding" author: Zhuge Jian), record the colony morphology, and investigate the physiological and biochemical characteristics of the strain according to the textbook "Common Bacterial System Identification Manual" (Author: Dong Xiuzhu)...

Embodiment 2

[0068] Embodiment 2: Bifidobacterium longum (Bifidobacterium longum) CCFM1109 and its fermentation supernatant affect the germination rate of filamentous fungal spores

[0069] 1. Effect of Bifidobacterium longum (Bifidobacterium longum) CCFM1109 on the germination rate of filamentous fungal spores (double-layer plate-growth inhibition method)

[0070] Pick the single bacterium colony of Bifidobacterium longum (Bifidobacterium longum) CCFM1109 screened in Example 1 and streak it on the mMRS solid medium, cultivate it at 37° C. for 48 hours in an anaerobic environment to obtain a single bacterium colony; pick a single bacterium colony and inoculate it in the mMRS liquid In the culture medium, culture was carried out at 37°C for 48 hours under anaerobic environment, and this operation was repeated 3 times to obtain the bacterial liquid cultivated to the third generation.

[0071] Use an inoculation loop to inoculate the Penicillium extensa liquid in the ampoule tube on the PDA m...

Embodiment 3

[0086] Embodiment 3: the influence of long Bifidobacterium longum (Bifidobacterium longum) CCFM1109 fermentation supernatant on the growth of filamentous fungus mycelium

[0087] Mix the mMRS liquid medium with the PDA medium at a volume ratio of 1:9, 1.5:8.5, 2:8, 2.5:7.5, 3:7 (mMRS liquid medium: PDA medium) to obtain mMRS liquid medium Concentration is respectively the control group mixed solution of 10,15,20,25,30% (v / v); The fermentation supernatant that embodiment 2 obtains is respectively 1:9,1.5:8.5,2:8,2.5: The volume ratio of 7.5, 3:7 (fermentation supernatant: PDA medium) was mixed with PDA medium to obtain experimental groups with fermentation supernatant concentrations of 10, 15, 20, 25, 30% (v / v) respectively Mixed solution; Pour the mixed solution of the control group and the experimental group into the plate respectively; add 10 μL of the Penicillium expanse spore suspension obtained in Example 2 dropwise to the center of the plate, and cultivate it at 28°C for...

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Abstract

The invention relates to bifidobacterium longum and application thereof in inhibiting filamentous fungi, and belongs to the technical field of biology. The invention provides bifidobacterium longum CCFM1109, and the strain can inhibit the filamentous fungi as follows: after being co-cultured with penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores, the strain can significantly inhibit the germination rate of the penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores separately within 7 days; after a fermented supernatant of the strain is co-cultured with the penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores, germination of the penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores can be significantly inhibited, and the spore germination inhibition rates can reach 98.38 + / - 0.37%, 90.23+ / - 0.67%, 92.65+ / - 1.27%, 96.34+ / - 0.53% respectively.

Description

technical field [0001] The invention relates to a bifidobacterium longum and its application in inhibiting filamentous fungi, belonging to the field of biotechnology. Background technique [0002] Studies have shown that from the initial planting to the final household consumption, there are different degrees of losses in the entire supply chain of fruits and vegetables. These losses are mainly caused by corruption, machinery problems such as damage and fruit fly infestation. Among them, spoilage is mainly caused by fungi, which can lead to appearance defects and nutritional deficiencies of fruits and vegetables. In developing countries with inadequate refrigeration and transportation facilities, the problem of fruit and vegetable spoilage is particularly prominent, and the losses it causes to the fruit and vegetable industry are also particularly serious. [0003] Penicillium extensa is a filamentous fungus belonging to the genus Penicillium, which can infect fruits and v...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A23B7/155C12R1/01
CPCC12N1/20C12N1/14A23B7/155C12R2001/01C12N1/205A23V2400/533
Inventor 翟齐啸陈卫田丰伟乔楠桢于雷雷赵建新张灏
Owner JIANGNAN UNIV
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