Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A Bifidobacterium adolescentis capable of inhibiting filamentous fungi and its application

A technology of bifidobacteria and filamentous fungi, applied in the field of Bifidobacterium adolescentis, can solve the problems of short duration of stay, poor biological control method, unsustainable physical control method, etc., and achieve the effect of good acid stability

Active Publication Date: 2022-03-25
JIANGNAN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the physical control method is mainly to inhibit the infection of filamentous fungi by microwave heating and cold storage of fruits and vegetables. However, due to the short time between the physical means such as microwave heating and cold storage on the surface of fruits and vegetables, the physical control method is not sustainable.
[0006] The biological control method mainly inhibits filamentous fungal infection by antagonizing microorganisms. Compared with physical control methods, this method is more sustainable. However, the biological control method has the disadvantage of poor effect, mainly because many antagonistic microorganisms themselves belong to fungi. , can also cause fungal spoilage on the fruit surface

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A Bifidobacterium adolescentis capable of inhibiting filamentous fungi and its application
  • A Bifidobacterium adolescentis capable of inhibiting filamentous fungi and its application
  • A Bifidobacterium adolescentis capable of inhibiting filamentous fungi and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Screening, identification, culture and observation of Bifidobacterium adolescentic CCFM1108

[0049] 1. Filter

[0050] Take 1 g of fecal samples from healthy adults in Wuxi, dilute them with normal saline, spread them on mMRS solid medium, and place them in an anaerobic environment at 37 °C for 72 hours, observe and record the colony morphology; pick colonies in mMRS The solid medium was streaked, purified and cultured in an anaerobic environment at 37°C to obtain a single colony after purification; a single colony was picked and streaked on the mMRS solid medium, anaerobic cultured at 37°C for 48h, and the obtained colonies were subjected to tanning. Lan's staining (for the Gram staining method, refer to the textbook "Industrial Microbial Breeding" by Zhuge Jian), record the colony morphology, and investigate the physiological and biochemical characteristics of the strains according to the textbook "Common Bacterial System Identification Manual" (author: Do...

Embodiment 2

[0065] Example 2: Effects of Bifidobacterium adolescentic CCFM1108 and its fermentation supernatant on the spore germination rate of filamentous fungi

[0066] 1. The effect of Bifidobacterium adolescentic CCFM1108 on the spore germination rate of filamentous fungi (double-layer plate-growth inhibition method)

[0067] Pick a single colony of Bifidobacterium adolescentic CCFM1108 screened in Example 1, streak on the mMRS solid medium, and culture at 37°C for 48h in an anaerobic environment to obtain a single colony; pick a single colony and inoculate it in the mMRS liquid In the culture medium, the bacteria were cultured at 37° C. for 48 hours in an anaerobic environment, and the operation was repeated 3 times to obtain the bacterial liquid cultured to the third generation.

[0068]Dip the Penicillium sp. liquid in the ampoule tube with an inoculation loop and inoculate it on the PDA medium, cultivate at 28° C. and 200 rpm for 7 days to obtain mycelium and spores; Repeat this...

Embodiment 3

[0083] Example 3: Effect of Bifidobacterium adolescentic CCFM1108 fermentation supernatant on the growth of Penicillium extensa

[0084] The mMRS liquid medium was mixed with the PDA medium in the volume ratios of 1:9, 1.5:8.5, 2:8, 2.5:7.5, 3:7 (mMRS liquid medium: PDA medium) to obtain mMRS liquid medium Concentrations were respectively 10, 15, 20, 25, 30% (v / v) of the control group mixed liquor; the fermentation supernatant obtained in Example 2 was 1:9, 1.5:8.5, 2:8, 2.5: The volume ratio of 7.5, 3:7 (fermentation supernatant: PDA medium) was mixed with PDA medium to obtain the experimental group with fermentation supernatant concentration of 10, 15, 20, 25, 30% (v / v) respectively Mixed liquid; pour the mixed liquid of the control group and the experimental group into the plate respectively; drop 10 μL of the Penicillium expansica spore suspension obtained in Example 2 in the center of the plate, and cultivate at 28 ° C for 6 days. The mycelium diameter of Bifidobacterium...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
control rateaaaaaaaaaa
Login to View More

Abstract

The invention discloses a bifidobacterium adolescent capable of inhibiting filamentous fungi and its application, belonging to the field of biotechnology. The present invention provides a strain of Bifidobacterium juvenileum CCFM1108, which can inhibit filamentous fungi, and is specifically embodied in: the bacterial strain is respectively mixed with Penicillium expanses spores, Aspergillus niger spores, Penicillium loudii spores or Penicillium digitatum spores After culturing, the germination rate of Penicillium expansica, Aspergillus niger, Penicillium loudi or Penicillium digitatum spores can be significantly inhibited within 7 days; After the co-cultivation of Penicillium spores or Penicillium digitatum spores, the germination of Penicillium sp. spores, Aspergillus niger spores, Penicillium loupeii spores or Penicillium digitatum spores can be significantly inhibited, and the spore germination inhibition rates can be as high as 99.88±0.66, respectively. %, 92.23±0.53%, 88.83±0.82%, 99.25±0.62%.

Description

technical field [0001] The invention relates to a Bifidobacterium adolescentis strain capable of inhibiting filamentous fungi and its application, belonging to the field of biotechnology. Background technique [0002] Fruit and vegetable spoilage can lead to cosmetic defects and nutritional deficiencies in fruits and vegetables. In developing countries with imperfect refrigeration and transportation facilities, the problem of fruit and vegetable corruption is particularly prominent, and its losses to the fruit and vegetable industry are also particularly serious. [0003] Filamentous fungi such as Penicillium extensa, Aspergillus niger, Penicillium dactylus, and Penicillium finger are one of the main culprits of the corruption of fruits and vegetables. They mainly cause corruption of fruits and vegetables by eroding the leaves and fruits of fruits and vegetables, thereby causing storage diseases of fruits and vegetables. , and they produce toxins (eg, patulin and citrinin),...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/01
CPCC12N1/20A23B7/155C12R2001/01C12N1/205A23V2400/513
Inventor 田丰伟陈卫翟齐啸乔楠桢于雷雷闫博文赵建新张灏
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com