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A strain of Bifidobacterium longum and its application in inhibiting filamentous fungi

A technology of Bifidobacterium longum and filamentous fungi, applied in the direction of bifidobacteria, applications, fungi, etc., can solve the problems of short residence time, non-sustainable physical control methods, and poor effects of biological control methods, achieving Effect of good acid stability

Active Publication Date: 2021-11-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the physical control method is mainly to inhibit the infection of filamentous fungi by microwave heating and cold storage of fruits and vegetables. However, due to the short time between the physical means such as microwave heating and cold storage on the surface of fruits and vegetables, the physical control method is not sustainable.
[0007] The biological control method mainly inhibits filamentous fungal infection by antagonizing microorganisms. Compared with physical control methods, this method is more sustainable. However, the biological control method has the disadvantage of poor effect, mainly because many antagonistic microorganisms themselves belong to fungi. , can also cause fungal spoilage on the fruit surface

Method used

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  • A strain of Bifidobacterium longum and its application in inhibiting filamentous fungi
  • A strain of Bifidobacterium longum and its application in inhibiting filamentous fungi
  • A strain of Bifidobacterium longum and its application in inhibiting filamentous fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Bifidobacterium longum (Bifidobacterium longum) screening, identification, culture and observation

[0052] 1 screening

[0053] 1g taken from healthy adult Wuxi in stool samples, after diluted with saline gradient coating on a solid medium mMRS, anaerobic conditions at 37 ℃ 72h culture, colony morphology observed and recorded; Colonies were picked in mMRS streaked onto solid medium and purified cultured at 37 [deg.] C in anaerobic environment, the purified single colonies; single colony was picked mMRS streaked on solid medium, 37 [deg.] C anaerobic culture 48h, the resulting colonies were leather Gram stain (Gram stain method reference textbook "industrial Microbiology breeding" author: Zhuge Jian forward), recorded colony morphology, and according to the textbook "common bacterial systems identification Handbook" (author: East Xiuzhu) physiological and biochemical characteristics of the study strains reserved Gram positive colonies are smooth, rounded, catalas...

Embodiment 2

[0068] Example 2: Effect of Bifidobacterius CCFM1109 and its fermentation supernatant on spore rate of filamentous fungus

[0069] 1. Effect of Bifidobacterius CCFM1109 on spore rate of filamentous fungus (double flat plate - growth inhibition)

[0070] Picking Example 1 Single Silition of the Bifidobacterius CCFM1109 of Example 1 was discharged from the MMRS solid medium, and the anaerobic environment was cultured at 37 ° C for 48 h, resulting in a single colony; picking a single bacterius into the MMRS liquid In the medium, 37 ° C in an anaerobic environment was cultured for culture, and this operation was repeated 3 times to obtain a bacterial liquid culture to the third generation.

[0071] The expanded pyrectomer in the inoculated ring was inoculated into PDA medium, 28 ° C, 200 rpm culture 7d, resulting in mycelial body and spores; picking spores on the PDA slope, 28 ° C, 200 rpm culture 7d, This operation was repeated 2 times to obtain a third generation of extension pupil;...

Embodiment 3

[0086] Example 3: Effect of Bifidobacterius CCFM1109 Fermented Supermourization on Growth of Filamentous Fungal Fungi

[0087] MMRS liquid medium was mixed with PDA medium in 1: 9, 1.5: 8.5, 2: 8, 2.5: 7.5, 3: 7 (MMRS liquid medium: PDA medium), respectively. The concentration of 10, 15, 20, 20, 30% (v / v) control group mixed liquid; the fermentation supernatant obtained in Example 2 was 1: 9, 1.5: 8.5, 2: 8, 2.5, respectively: The volume ratio of 7.5, 3: 7 (fermented supernatant: PDA medium) is mixed with PDA medium to obtain an experimental group of 10, 15, 20, 25, 30% (V / V), respectively. The mixture was mixed; the mixture of the control group and the experimental group was poured; 10 μl of the expanded Popular spore suspension obtained by adding 10 μl of Example 2, at 28 ° C, during culture, and measured each tablet through each 2D measurement The fungus diameter, detecting the effect of Bifidobacterium CCFM1109 fermentation supernatant on the growth of the polyfusions, the...

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Abstract

The invention relates to a bifidobacterium longum and its application in inhibiting filamentous fungi, belonging to the field of biotechnology. The present invention provides a strain of Bifidobacterium longum CCFM1109, which can inhibit filamentous fungi, and is specifically embodied in that the bacterial strain is respectively mixed with spores of Penicillium expanses, Aspergillus niger, spores of Penicillium loudii or Penicillium digitatum. After culturing, the germination rate of Penicillium expansica, Aspergillus niger, Penicillium loudi or Penicillium digitatum spores can be significantly inhibited within 7 days; After the co-cultivation of Penicillium spores or Penicillium digitatum spores, the germination of Penicillium sp. spores, Aspergillus niger spores, Penicillium loupeii spores or Penicillium digitatum spores can be significantly inhibited, and the spore germination inhibition rate can be as high as 98.38±0.37 %, 90.23±0.67%, 92.65±1.27%, 96.34±0.53%.

Description

Technical field [0001] The present invention relates to a Bifidobacterium longum and Its Application in suppressing filamentous fungi, the field of biotechnology. Background technique [0002] Studies have shown that, from planting the beginning to the final household consumption, supply chain throughout fruits and vegetables are varying degrees of losses, these losses are mainly corruption of fruits and vegetables that occur in the process after the mining, transportation, storage, mechanical fruit fly infestation and damage and other issues. Where corruption is mainly caused by the fungus, which can lead to the appearance of defects and lack of nutrition of fruits and vegetables. In the imperfect cold storage and transport facilities in developing countries, especially fruits and vegetables corruption problem, which caused the loss of the fruit and vegetable industry is particularly serious. [0003] Penicillium expansum filamentous fungi belonging to the genus Penicillium, whi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14A23B7/155C12R1/01
CPCC12N1/20C12N1/14A23B7/155C12R2001/01C12N1/205A23V2400/533
Inventor 翟齐啸陈卫田丰伟乔楠桢于雷雷赵建新张灏
Owner JIANGNAN UNIV
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