A methylotrophic bacillus strain for biocontrol and application thereof

A methylotrophic, bacillus technology, applied in the application, bacteria, microorganisms and other directions, can solve the problem of less volatile substances being reported, and achieve the effects of convenient industrial production and transportation, wide antibacterial spectrum and simple production process

Active Publication Date: 2019-01-11
ZHEJIANG UNIV
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Problems solved by technology

However, little has been reported on the vola...

Method used

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  • A methylotrophic bacillus strain for biocontrol and application thereof
  • A methylotrophic bacillus strain for biocontrol and application thereof
  • A methylotrophic bacillus strain for biocontrol and application thereof

Examples

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Embodiment Construction

[0031] PDA medium: 200g peeled potato, 20g glucose, 20g agar, 1000mL water.

[0032] LB medium: 10g sodium chloride, 10g tryptone, 5g yeast extract, 20g agar, 1000 mL water.

[0033] (1) In vitro inhibitory effect of volatile substances of methylotrophic Bacillus BCN2 on common loquat spoilage bacteria:

[0034] 1. Methylotrophic Bacillus Bacillus methylotrophicusBCN2 for biological control, the identification results are as follows figure 1 , has good in vitro antifungal activity.

[0035] 2. Preparation of methylotrophic Bacillus for biocontrol

[0036] Activation and preparation: Cultivate methylotrophic Bacillus for biocontrol in LB medium at 32°C for 12h. Then repeat the subculture twice, use an inoculation loop to pick an appropriate amount of bacteria into a 250 mL Erlenmeyer flask filled with 50 mL of LB liquid medium, vibrate and culture in a shaker at 150 rpm and 32 °C for 12 hours, and dilute the fermentation broth appropriately so that the bacterial concentratio...

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Abstract

A methylotrophic bacillus strain (Bacillus methylotrophicus) for biocontrol and application thereof are disclosed. The bacterium Bacillus methylotrophicus BCN2 is deposited in the China General Microbiological Culture Collection Center under the accession number CGMCC No: 16443. The invention comprises isolation and culture of the strain and molecular identification of 16S rDNA; effective inhibiting effects of Methylotrophic Bacillus volatiles against common spoilage fungi (Fusarium oxysporum, Staphylococcus aureus, Trichoderma viride, Colletotrichum gloeosporioides, Penicillium expansum) ontwo-zone plate and loquat fruits and the antiseptic application of the volatile matter in loquat storage. The volatile antimicrobial substances produced by Bacillus methylotrophicus (BCN2) could not contact fruit directly, It can be used for preparing biocontrol fresh-keeping agent which can inhibit spoilage fungi such as Fusarium oxysporum, Staphylococcus aureus, Trichoderma viride, Colletotrichum gloeosporioides, Penicillium expansum and so on, and has wide bacteriostasis spectrum.

Description

technical field [0001] The invention relates to a class of volatile antibacterial substances produced by Bacillus methylotrophicus BCN2 and the biotechnical field of preventing and treating post-harvest loquat diseases, belonging to the technical field of food preservation. Background technique [0002] The huge loss caused by postharvest diseases of fruits has become a concern at home and abroad. In developed countries, about 10%-30% of fresh fruits are lost due to rot due to postharvest diseases, and postharvest rot is more serious in developing countries. At present, the prevention and control methods of postharvest diseases of fruits mainly include chemical fungicides, controlled atmosphere storage, low temperature disease prevention, heat treatment, etc. Among them, chemical antisepsis has the best effect. Biological control does not have the problems of environmental pollution, pesticide residues and continuous use of chemical resistance caused by chemical antiseptic p...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23B7/155C12R1/07
CPCA23B7/155C12N1/20C12N1/205C12R2001/07
Inventor 周文文何超男孙亚芳
Owner ZHEJIANG UNIV
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