Organic pollution water body bioremediation agent and preparation method thereof

A bioremediation agent and organic pollution technology, applied in biochemical equipment and methods, methods based on microorganisms, water pollutants, etc., can solve the problems of low decomposition rate, single strain, single technology, etc., to achieve increased compactness, The effect of improving water quality

Inactive Publication Date: 2013-10-16
上海玉仑应用微生物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its preparation cost is high, production capacity is low, liquid bacterial agent has a short validity period in the engineering interface, poor stability, harsh storage conditions, and excessive transportation consumption in engineering applications
Lack of industrialization prospects, the most fatal flaw is that the natural properties of the strains of the liquid bacterial agent remain unchanged, and cannot fundamentally solve the problems of weak stain resistance, poor environmental tolerance and low decomposition rate
[0003] In the treatment of organically polluted water bodies, the domestic technology is single and the strains are single, and foreign bacterial agents cannot adapt to the characteristics of the Chinese environment, and there are problems such as localization and security inspection.
Moreover, the existing biotechnology in China relies on the spraying of laboratory-level liquid bacteria solution, which can only maintain the effect in the short term, and requires uninterrupted spraying for long-term maintenance. Moreover, the water body has low anti-pollution ability and self-purification ability, and the water quality is easy to repeat.

Method used

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  • Organic pollution water body bioremediation agent and preparation method thereof
  • Organic pollution water body bioremediation agent and preparation method thereof
  • Organic pollution water body bioremediation agent and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A bioremediation agent for organically polluted water bodies, the raw materials include the following components and their weight percentages: 0.6% of Aspergillus oryzae bacteria liquid, 0.6% of Bacillus cereus bacteria liquid, 0.6% of Aspergillus terreus bacteria liquid, 0.6% of Aspergillus nidulans bacteria liquid, 50% of rice husk, the balance is natural river water, and the bacteria content in the above bacteria liquid is 1×10 8 ~9×10 12 pieces / ml.

[0037] The preparation method of organic polluted water bioremediation agent comprises the following steps:

[0038] (1) Preparation of Aspergillus oryzae liquid:

[0039] a. Prepare Aspergillus oryzae culture medium raw materials, including the following components and weight percentages: agar 1.4-1.6%; 5° wort 98.4-98.6%;

[0040] B, the preparation of liquid culture medium: prepare raw material according to the aspergillus oryzae medium formula, at 0.15MPa, 121 ℃, sterilize 15min, obtain the sterilized aspergillus...

Embodiment 2

[0060] A bioremediation agent for organically polluted water bodies, the raw materials include the following components and their weight percentages: 0.8% of Aspergillus oryzae bacteria liquid, 0.8% of Bacillus cereus bacteria liquid, 0.8% of Aspergillus terreus bacteria liquid, 0.8% of Aspergillus nidulans bacteria liquid, 50% of rice husk, the balance is natural river water, and the bacteria content of the above-mentioned bacteria liquid is 1×10 8 ~9×10 12 pieces / ml.

[0061] The preparation method of organic polluted water bioremediation agent comprises the following steps:

[0062] (1) Preparation of Aspergillus oryzae liquid:

[0063] a. Prepare Aspergillus oryzae culture medium raw materials, including the following components and weight percentages: agar 1.4-1.6%; 5° wort 98.4-98.6%;

[0064] B, the preparation of liquid culture medium: prepare raw material according to the aspergillus oryzae medium formula, at 0.15MPa, 121 ℃, sterilize 15min, obtain the sterilized a...

Embodiment 3

[0084] A bioremediation agent for organically polluted water bodies, the raw materials include the following components and weight percentages: 1.0% of Aspergillus oryzae bacteria liquid, 1.0% of Bacillus cereus bacteria liquid, 1.0% of Aspergillus terreus bacteria liquid, 1.0% of Aspergillus nidulans bacteria liquid, 60% of rice husk, the balance is natural river water, and the bacteria content of the above-mentioned bacteria liquid is 1×10 8 ~9×10 12 pieces / ml.

[0085] The preparation method of organic polluted water bioremediation agent comprises the following steps:

[0086] (1) Preparation of Aspergillus oryzae liquid:

[0087] a. Prepare Aspergillus oryzae culture medium raw materials, including the following components and weight percentages: agar 1.4-1.6%; 5° wort 98.4-98.6%;

[0088] B, the preparation of liquid culture medium: prepare raw material according to the aspergillus oryzae medium formula, at 0.15MPa, 121 ℃, sterilize 15min, obtain the sterilized aspergi...

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Abstract

The present invention relates to an organic pollution water body bioremediation agent and a preparation method thereof. The raw materials comprise, by weight, 0.6-1.0% of an Aspergillus oryzae liquid, 0.6-1.0% of a Bacillus cereus liquid, 0.6-1.0% of an Aspergillus terreus liquid, 0.6-1.0% of an Aspergillus nidulans liquid, 50-60% of hull, and the balance of nature river water, wherein mixing, fermentation, and acclimation preparation are performed to obtain the organic pollution water body bioremediation agent. Compared with the organic pollution water body bioremediation agent in the prior art, the organic pollution water body bioremediation agent of the present invention has the following characteristics that: black, odorous and eutrophicated organic pollutants are formed in a water body so as to be decomposed and be discharged out of the water, such that water quality is improved; and other technologies such as aquatic plants and animals, and the like are combined, such that the polluted water body can be restored to the ground surface IV-V class water.

Description

technical field [0001] The invention relates to a bioremediation agent and a preparation method thereof, in particular to an organic polluted water bioremediation agent and a preparation method thereof. Background technique [0002] It is known that the bioaugmentation (introduction) process that has emerged internationally is based on the principle of using biological culture methods to select a single bacterial species, prepare it into a liquid preparation with a high bacterial content, and put it into the engineering interface to strengthen the biodegradation process. strength. However, its preparation cost is high, production capacity is low, the validity period of the liquid bacterial agent in the engineering interface is short, the stability is poor, and the storage conditions are harsh, and the transportation consumption of the engineering application is too large. The lack of industrialization prospects, the most fatal flaw is that the natural properties of the stra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/14C12N1/20C12R1/69C12R1/085C12R1/66C02F101/30
Inventor 钱臻
Owner 上海玉仑应用微生物研究所
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