Primer and probe of invasive mucor pathogenic bacteria, implementation method and detection system thereof

Abstract

The invention discloses a primer and a probe of invasive mucor pathogenic bacteria, an implementation method and a detection system thereof. The method comprises the following steps of: comparing whole genome data of all strains of the obtained invasive mucor pathogenic bacteria to obtain a plurality of conserved genes of the invasive mucor pathogenic bacteria; and designing a universal primer and a probe of the invasive mucor pathogenic bacteria with specificity meeting the requirement and a species-specific primer and a probe of all strains of the invasive mucor pathogenic bacteria by utilizing the plurality of conserved genes of the invasive mucor pathogenic bacteria obtained by whole genome data comparison. By utilizing the primer and the probe designed by the invention, the sensitivity and the specificity of detection of the invasive mucor pathogenic bacteria can be improved, and various strains including aspergillus fumigatus, aspergillus flavus, aspergillus terreus, aspergillus niger and aspergillus nidulans can be detected; and the primer and the probe are suitable for different clinical samples such as BALF and FFPE tissues and exudates (pus).

Description

technical field

[0001] The invention relates to a primer and a probe for invasive mucormycosis pathogenic bacteria, a realization method and a detection system thereof. Background technique

[0002] In recent years, the prevalence of invasive mucormycosis has been increasing, the course of the disease has progressed rapidly, and the mortality rate can reach 40%-80%. The most common pathogenic species are Rhizopus, Mucor, Rhizopus and Rhizomucor.

[0003] Early diagnosis is helpful for precise treatment and improved prognosis. However, the sensitivity and specificity of commonly used clinical methods for the detection of invasive mucormycosis pathogens are low, and it is difficult to quickly and accurately detect the pathogenic bacteria in different clinical samples.

[0004] In the existing real-time fluorescence quantitative PCR (Quantitative Real-time PCR, qPCR) method for the diagnosis of mucormycosis, the target genes are set at 18s, 28s, and mitochondrial genes, and t...

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