Method for preparing straw-decomposing inoculant capable of inhibiting soil-borne diseases
A technology of straw decomposing agent and soil-borne diseases, which is applied in the field of agricultural microbiology and can solve problems such as water pollution
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[0037] Below in conjunction with specific embodiment, further illustrate the present invention.
[0038] Bacteria production is achieved through the following examples
[0039] 1. Inoculate Aspergillus nidulans into the PDA culture medium, and carry out liquid fermentation production. The conditions of its fermentation production are: initial pH is natural, culture temperature is 25°C, dissolved oxygen: ventilation range is 30-100%, 170rpm, fermentation is in progress Mycelial fragments are formed in the later stage, and the colony forming unit of the fermentation broth strain is ≥1×10 7 cfu ml -1 The PDA nutrient solution preparation method used is (taking the preparation of 1L medium as an example): after peeling 200g potatoes, it is cut into small pieces and put into water to boil, after boiling, boil 30min, after filtering with gauze, the filtrate adds 20g common sucrose, Dilute to 1000ml and sterilize at 121°C for 20min. The fermentation broth of Aspergillus nidulans i...
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