Antagonistic yeast Candida anatomiae as well as preparation and application method thereof

A technology of Candida and strains, applied in biochemical equipment and methods, methods based on microorganisms, applications, etc., can solve the problems of lack of antibacterial spectrum strains, biocontrol effects are only verified on a small number of fruits, etc., to achieve Significant social and ecological benefits, low cost of use, and good growth effects

Active Publication Date: 2018-04-20
北京华康信使科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, although there are nearly a hundred species of antagonistic yeasts reported at home and abroad, the biocontrol effects of most antagonistic yeasts have only been verified on a few fruits.
However, since the biocontrol effects of different strains of the same yeast are very different (Filonow et al., 1996), most of the antagonistic yeasts lack strains with broad antibacterial spectrum and stable effect.

Method used

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  • Antagonistic yeast Candida anatomiae as well as preparation and application method thereof
  • Antagonistic yeast Candida anatomiae as well as preparation and application method thereof
  • Antagonistic yeast Candida anatomiae as well as preparation and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Biological properties of Candida anatomiae strain BY13

[0023] 1. Morphological characteristics

[0024] (1) YPDA medium (1% yeast extract powder, 2% peptone, 2% glucose, 1.8% agar, sterilized at 121°C for 20 minutes) was cultured at 26°C for 48h, and the colonies were round and white with smooth and round edges. The cell shape is ellipsoidal.

[0025] (2) After culturing in YPDA liquid medium for 24 hours, no mold was formed, the bacterial solution was turbid, and there was precipitation. Microscopically, the yeast cells were oval and budded.

[0026] 2. Molecular biological identification

[0027] Use the universal forward primer NL-1 (5'-GCATATCAATAAGCGGAGGAAAAG-3') and reverse primer NL-4 (5'-GGTCCGTGTTTCAAGACGG-3') to perform PCR amplification of yeast 26S rDNA D1 / D2 region nucleic acid sequence, and the PCR product The sequencing results were entered into the website www.NCBI.nlm.nih.gov for BLAST, the homologous sequences were downloaded from the G...

Embodiment 2

[0029] The inhibitory effect of implementation example 2 Candida BY13 to apple penicillium and botrytis cinerea

[0030] 1. Experimental protocol

[0031] Candida BY13 was taken out from the -80°C refrigerator, activated with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and picked a single Colony into YPD liquid medium, culture at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on a hemocytometer to prepare the concentration 1×10 8 cells / mL of Candida BY13 suspension.

[0032] Activate Penicillium expansum or Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water.4 cells / mL of Penicillium or Botrytis cinerea spore suspension. Healthy and undamage...

Embodiment 3

[0039] The inhibitory effect of implementation example 3 Candida BY13 on pear fruit blue mold and gray mold

[0040] 1. Experimental protocol

[0041] Candida BY13 was taken out from the -80°C refrigerator, activated with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and picked a single Colony into YPD liquid medium, culture at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on a hemocytometer to prepare the concentration 1×10 8 cells / mL of Candida BY13 suspension.

[0042] Activate Penicillium expansum or Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water. 4 cells / mL of Penicillium or Botrytis cinerea spore suspension. Healthy and undamaged ...

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Abstract

The invention discloses a Candida anatomiae bacterial strain BY13 used for control of postharvest diseases of fruits and vegetables and with broad antibacterial spectrum and stable effect and an application method thereof. The bacterial strain is preserved in CGMCC with the preservation number of CGMCC No.14901. The application method of the candida mycoderma bacterium comprises the following steps: activating the bacterial strain, carrying out fermental cultivation with YPD, centrifuging, and preparing bacterium suspension with the concentration of 1*10<8> cells / mL from thalli with sterile water; putting fruits and vegetables such as apples, pears, grapes, strawberries, citrus and cherry tomatoes into the bacterium suspension, soaking for 30 seconds, then taking out, and carrying out airdrying; and putting into a preservation box, and storing at room temperature. The Candida anatomiae bacterial strain disclosed by the invention can control penicilliosis and botrytis of the apples, penicilliosis and botrytis of the pears, botrytis, anthracnose, aspergillosis, black spot and fusariosis of the grapes, botrytis and aspergillosis of the strawberries, penicilliosis of the citrus as well as botrytis and aspergillosis of the cherry tomatoes at the same time, and loss caused by the postharvest diseases is reduced, so that the Candida anatomiae bacterial strain disclosed by the invention has a good application prospect.

Description

technical field [0001] The invention relates to the field of biological control of postharvest diseases of fruits and vegetables, in particular to a strain of Candida anatomiae used for biological control of postharvest diseases of fruits and vegetables. The main postharvest diseases have significant control effects. Background technique [0002] The quality deterioration of fresh fruit and vegetable products is affected by many factors, but disease is the main reason. Among them, rot and deterioration caused by fungal diseases is the most serious factor in postharvest fruit loss. Although postharvest diseases of fruits and vegetables can be controlled through many methods such as agricultural control, physical control, chemical control and biological control, the main measure to control postharvest diseases of fruits and vegetables is chemical control (Eckert & Ogawa, 1985, 1988). Today, people are paying more and more attention to pollution-free food. While fresh fruit o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23B7/155C12R1/72
CPCA23B7/155C12N1/16C12N1/165C12R2001/72
Inventor 王友升范雅为陈滢姚婷黄津津马琳
Owner 北京华康信使科技有限公司
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