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Application of a strain of Candida in the control of postharvest diseases of fruits

A technology for postharvest diseases of fruits and Candida, which is applied in the fields of application, preservation of fruits and vegetables, and methods based on microorganisms, can solve the problems of lack of bacteriostatic spectrum strains, and the biocontrol effect is only verified on a few fruits. Achieve significant social and ecological benefits, avoid harm to people, and be easy to cultivate.

Active Publication Date: 2021-03-09
山东凯普菲特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although there are nearly a hundred species of antagonistic yeasts reported at home and abroad, the biocontrol effects of most antagonistic yeasts have only been verified on a few fruits.
However, since the biocontrol effects of different strains of the same yeast are very different (Filonow et al., 1996), most of the antagonistic yeasts lack strains with broad antibacterial spectrum and stable effect.

Method used

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  • Application of a strain of Candida in the control of postharvest diseases of fruits
  • Application of a strain of Candida in the control of postharvest diseases of fruits
  • Application of a strain of Candida in the control of postharvest diseases of fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Biological properties of Candida railenensis BY16 strain

[0021] 1. Morphological features

[0022] (1) YPDA medium (1% yeast extract powder, 2% peptone, 2% glucose, 1.8% agar, sterilized at 121°C for 20 minutes) was cultured at 26°C for 48h, and the colonies were round and white with smooth and round edges. The cell shape is ellipsoidal.

[0023] (2) After culturing in YPDA liquid medium for 24 hours, no mold was formed, the bacterial solution was turbid, and there was precipitation. Microscopically, the yeast cells were oval and budded.

[0024] 2. Molecular biological identification

[0025] Use the universal forward primer NL-1 (5'-GCATATCAATAAGCGGAGGAAAAG-3') and the reverse primer NL-4 (5'-GGTCCGTGTTTCAAGACGG-3') to PCR amplify the yeast 26S rDNA D1 / D2 region nucleic acid sequence, and PCR The sequencing results of the product were entered into the website www.NCBI.nlm.nih.gov for BLAST, the homologous sequences were downloaded from the GenBank data...

Embodiment 2

[0027] Example 2 Inhibition of Candida BY16 to Apple Penicillium and Botrytis Botrytis

[0028] 1. Experimental protocol

[0029] Candida BY16 was taken out from the -80°C refrigerator, activated with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and picked a single Colony into YPD liquid medium, culture at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on a hemocytometer to prepare the concentration 1×10 8 cells / mL of Candida BY16 suspension.

[0030] Activate Penicillium expansum or Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water. 4 cells / mL of Penicillium or Botrytis cinerea spore suspension.

[0031]Healthy and undamaged apple fruits ...

Embodiment 3

[0038] Example 3 Inhibitory effect of Candida BY16 on pear fruit blue mold and gray mold

[0039] 1. Experimental protocol

[0040] Candida BY16 was taken out from the -80°C refrigerator, activated with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and picked a single Colony into YPD liquid medium, culture at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on a hemocytometer to prepare the concentration 1×10 8 cells / mL of Candida BY16 suspension.

[0041] Activate Penicillium expansum or Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and use sterile water to prepare a concentration of 5×104 cells / mL of Penicillium or Botrytis cinerea spore suspension.

[0042] Disinfect healthy and undamaged...

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Abstract

The invention discloses Candida railenensis strain BY16 with wide antibacterial spectrum and stable effect for preventing and controlling postharvest diseases of fruits and vegetables and a use method thereof. The preservation strain number of the strain in the General Microorganism Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.14902. The method of using the Candida: activate the strain, ferment and cultivate it with YPD, centrifuge, and use sterile water to make 1×10 8 cells / mL bacterial suspension; put apples, pears, grapes, strawberries, citrus or cherry tomatoes and other fruits and vegetables into the bacterial suspension, soak for 30 seconds, take out, and air-dry; put them in a fresh-keeping box and store at room temperature. This Candida strain can simultaneously control apple blue mold and gray mold, pear blue mold and gray mold, grape gray mold, anthracnose, aspergillosis, black spot, fusarium and powdery mildew, Strawberry gray mold and aspergillosis, citrus penicillium, and cherry tomato gray mold and aspergillosis can reduce losses caused by postharvest diseases and have good application prospects.

Description

technical field [0001] The invention relates to the field of biological control of post-harvest diseases of fruits, in particular to a strain of Candida railenensis used for the biological control of post-harvest diseases of fruits. The main postharvest diseases have significant control effect. Background technique [0002] Although the deterioration of fresh fruit quality is affected by many factors, disease is the main reason. Among them, rot and deterioration caused by fungal diseases is the most serious factor in postharvest fruit loss. Although postharvest diseases of fruits can be prevented and controlled in many ways such as agricultural control, physical control, chemical control and biological control, the main measure at present is chemical control (Eckert & Ogawa, 1985, 1988). However, the long-term use of chemical pesticides not only leads to the development of drug resistance of pathogenic bacteria and reduces the bactericidal effect (Prusky et al., 1985; Viňa...

Claims

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Application Information

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IPC IPC(8): C12N1/16A23B7/155C12R1/72
CPCA23B7/155C12N1/16C12N1/165C12R2001/72
Inventor 王友升马琳陈滢李丽萍任向峰姚婷
Owner 山东凯普菲特生物科技有限公司
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