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Combined preparation method of garlic extract

A garlic extract and combined preparation technology are applied in the fields of separation and purification, and natural substance extraction, can solve problems such as single extraction components, and achieve the effects of simple operation, improved acceptance and low cost

Active Publication Date: 2021-04-27
青岛博恩高科生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical problem that the existing extraction process has a single extraction component and cannot fully utilize garlic raw materials, and proposes a combined preparation method of garlic extracts that is easy to operate, low in cost, high in raw material utilization, and high in extract yield

Method used

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  • Combined preparation method of garlic extract

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Effect test

preparation example Construction

[0040]Embodiments of the present invention provide a combination method of garlic extract, including the following steps:

[0041]S1, Garlic extract: Fresh garlic peeled cleaning after pulping in the solvent, filtered, produce garlic extract;

[0042]S2, macaroninase preparation: Separate the garlic extract using ultrafiltration membrane equipment to obtain trap and dialysate, the trays, dissolved, desalted, and freeze-drying, resulting in garlic Using aproxidase;

[0043]S3, 大 Preparation: After the dialysate is passed through a large pore resin, the maxored resin effluent is collected, and then elute the macroporous resin by eluent, collect the large pore resin elution, after concentration drying,得 大;

[0044]S4, garlic polysaccharide and garlic amino acid Preparation: After the spherical resin effluent is exchanged by cation, the 1-3 column volume is eluted with purified water, collecting cation exchange resin effluent and eluent, and regulates its pH To neutral, then concentrated drying, pr...

Embodiment 1

[0092]This embodiment provides a combination method of garlic extract, and the specific steps are as follows:

[0093](1) Garlic extract: add 3 times the mass of drinking water soaked for 1h, flowing water peel, add 5 times the mass of the garlic valve to 20 times the pulp to 200 destination garlic pulp, garlic pulp with reduced pressure Filter equipment is filtered, and the clear garlic extract is obtained;

[0094](2) Garlic extracts were isolated using an ultrafiltration membrane of 80,000 Dalton to obtain a trap and dialysate, of which the carinase is stirred in the trap in the trap. The precipitate was prepared by adding 30% ammonium sulfate to prepare a pemperidase precipitate with centrifuge, and the precipitate was dissolved with pH = 6.5, and the carinase solution was placed in a polyurethane bag, at 0-5 Dialysis of 48 h in the flow ice water tank was dialyzed by icy, and the pewer was obtained from the frozen dry 20-40H.

[0095](3) Osser prepared: The dialysate obtained by step (2...

Embodiment 2

[0099]This embodiment provides a combination method of garlic extract, and the specific steps are as follows:

[0100](1) Garlic extract: adding 3 times the quality drinking water soaked for 1h, flowing water peels, adding 4 times the garlic valve with 4 times the mass of the garrow to crush to 200 destination garlic pulp, garlic pulp with reduced pressure Filter equipment is filtered, and the clear garlic extract is obtained;

[0101](2) Garlic extracts were isolated using a 50,000 Dalton ultrafiltration membrane to obtain a trap and dialysate, of which the carinase is stirred in the trap in the trap. 40% ammonium sulfate was added to precipitate, and a glycinase precipitate was centrifuged with a centrifuge, and the precipitate was dissolved using a phosphate buffer of pH = 6.0, placed in a polyurethane bag, at 0-5 Dialysis of 48 h in the flow ice water tank was dialyzed by icy, and the pewer was obtained from the frozen dry 20-40H.

[0102](3) Large oculum: The dialysate obtained by step ...

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Abstract

The invention provides a combined preparation method of a garlic extract, belongs to the field of natural substance extraction, separation and purification, and can solve the problems of single component of a substance prepared by an extraction process and incapability of truly realizing full utilization of garlic raw materials in the prior art. The preparation method comprises the following steps of: (1) preparing a garlic extracting solution; (2) preparing alliinase; (3) preparing allicin; and (4) preparing garlic polysaccharide and garlic amino acid. The method can be applied to combined preparation of the garlic extract.

Description

Technical field[0001]The invention belongs to the field of natural material extraction, separation, and purification, and more particularly to a combination preparation method of garlic extract.Background technique[0002]Garlic (Allium Sativum L.) has a long history of medicinal food, and has a large number of studies. Garlic has a variety of antibacterial, anti-inflammatory, anti-thrombus, lowering blood fat, anti-tumor and enhance immunity. Garlic sugar , Protein, sulfur-containing organic compound, amino acid, etc., etc., also the material foundation of garlic to play a variety of biological effects.[0003]Alkoxide, but also catalyzes allyl disulfulfoxide, which can catalyzes allylisulfone, but also catalyzed, but also catalyzes allyl dikathulphazol sulfoxide, including garlic. Applying to tumor treatment, there is a broad application prospect in the biomedicine industry; uliocarin is a sulfurine-containing compound produced under alcohinase catalytic action, which can be further c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C381/00C07C315/06C07C317/48C12N9/88C08B37/00
CPCC07C381/00C07C315/06C07C317/48C12N9/88C12Y404/01004C08B37/0003Y02A50/30
Inventor 朱颖秋王鹏钟斌王庚申
Owner 青岛博恩高科生物技术有限公司
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