A kind of extraction and separation method of ajoene in garlic

A separation method and technology of ajoene, which is applied in the field of extraction and separation of garlic ajoene, can solve problems such as difficulty in obtaining ajoene products, large consumption of ethanol and vegetable oil, difficult recovery and utilization of solvents, etc., and achieve high utilization rate, The effect of short production cycle and easy operation

Active Publication Date: 2019-12-20
深圳市阿霍烯生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this method, it is difficult to obtain a relatively high content of ajoene products. In the presence of a large amount of water, the edible oil has problems such as high acid value and emulsification due to hydrolysis.
Chinese patent (ZL201510402394.8) extracts garlic with a solvent, mixes it with vegetable oil, and then uses an adsorbent to absorb and elute with a solvent to prepare ajoene products with a purity of 70% to 90%. However, this method has a large amount of ethanol and vegetable oil, and the production high cost problem
Chinese patent (publication number CN106518739A) passes through supercritical CO after crushing black garlic 2 Extraction-molecular distillation separation-preparative liquid phase purification to prepare ajoene samples with a purity of 68.13% to 72.35%, but the equipment used in this method is relatively expensive, the production cycle is long, energy consumption is high, and the solvent is difficult to recover and utilize and low purity

Method used

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  • A kind of extraction and separation method of ajoene in garlic
  • A kind of extraction and separation method of ajoene in garlic
  • A kind of extraction and separation method of ajoene in garlic

Examples

Experimental program
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Effect test

Embodiment 1

[0026] (1) Weigh 10 kg of pretreated Shandong garlic, pulverize with a grinder to a particle size of 1 mm, add 40L of 95% ethanol solution, and extract with ultrasonic vibration at room temperature for 1 hour. After the extraction is completed, filter with a bag, and the filtrate is concentrated under reduced pressure at 50-60℃ to remove the solvent. Concrete extract I. The recovered ethanol will be used for the next batch of extraction after inspection and meet the requirements, and the garlic residue will be used to extract polar components such as polysaccharides or to make feed;

[0027] (2) Put the extract I obtained in step (1) into the extraction kettle of the supercritical extraction device, using supercritical CO 2 Fluid extraction and separation. The extraction kettle is heated to 65°C and the pressure is increased to 40MPa, and the separation kettle I is heated to 50°C and the pressure is increased to 16MPa, and then cyclic extraction is started to control CO 2 The flo...

Embodiment 2

[0031] (1) Weigh 10 kg of pretreated Gansu garlic, pulverize with a grinder to a particle size of 0.2mm, add 20L of 95% ethanol solution, and extract with ultrasonic vibration at room temperature for 2 hours. After the extraction is completed, filter with a cloth bag, and the filtrate is concentrated and removed under reduced pressure at 50-60°C. The solvent is used to obtain extract II. The recovered ethanol will be used for the next batch of extraction after inspection and meet the requirements, and the garlic residue will be used to extract polar components such as polysaccharides or to make feed;

[0032] (2) Put the extract II obtained in step (1) into the extraction kettle of the supercritical extraction device, using supercritical CO 2 Fluid extraction and separation. The extraction kettle is heated to 75°C and the pressure is increased to 15MPa, and the separation kettle I is heated to 60°C and the pressure is increased to 20MPa, and then the cyclic extraction is started ...

Embodiment 3

[0036] (1) Weigh 10Kg of the pretreated Yunnan garlic, pulverize it to a particle size of 2mm, add 80L 95% ethanol solution, and extract with ultrasonic vibration at room temperature for 0.5h. After the extraction is completed, filter with a cloth bag, and the filtrate is concentrated and removed under reduced pressure at 50-60℃. The solvent is used to obtain the extract III. The recovered ethanol will be used for the next batch of extraction after inspection and meet the requirements, and the garlic residue will be used to extract polar components such as polysaccharides or to make feed;

[0037] (2) Put the extract III obtained in step (1) into the extraction kettle of the supercritical extraction device, using supercritical CO 2 Fluid extraction and separation. The extraction kettle is heated to 35°C and the pressure is increased to 60MPa, and the separation kettle I is heated to 30°C and the pressure is increased to 9MPa, and then cyclic extraction is started to control CO 2 ...

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Abstract

The invention belongs to the technical field of extraction and separation of effective components of traditional Chinese medicines, and discloses an extraction and separation method of ajoene in garlic. According to the method, by utilizing an ultrasonic technology, the garlic is preliminarily treated by taking ethyl alcohol as a solvent, and the lower-content ajoene in the garlic is extracted and enriched from raw materials within a short time, so as to improve the efficiency for further extraction and separation; secondary extraction, refinement and separation are performed by utilizing the characteristic that CO2 has a selective dissolving capacity for substances with different polarities such as the ajoene under a super-critical state, and the purity of an ajoene product obtained finally is greater than 80 percent. The novel method provided by the invention overcomes the defects of the existing preparation method, has the advantages of short production period, environmental friendliness, high ajoene purity, high utilization rate of raw material medicine residue and the like, and can be used for large-batch industrial production.

Description

Technical field [0001] The invention relates to the technical field of extraction of Chinese medicine components, in particular to an extraction and separation technology of garlic ajoene. Background technique [0002] Ajoene is a highly biologically active sulfide produced by the conversion of alliin and allinase in garlic. According to research, it has the physiological and pharmacological activities of preventing cardiovascular diseases, anti-oxidation, anti-thrombosis, lowering blood pressure, reducing the incidence of Alzheimer's disease and cancer, as well as anti-bacterial, anti-viral and improving immunity. [0003] Garlic is rich in alliin, which mainly exists in the garlic cytoplasm in a stable and odorless form. Alliin is the precursor substance of the main functional components in garlic, which can produce allicin, pyruvate and ammonia under the catalytic cleavage of allinase. In cutting, mashing, chewing, etc., Alliinase will quickly decompose alliin into thiosulfina...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C319/28C07C323/65
CPCC07C319/28C07C323/65Y02P20/54
Inventor 黄伟昌张佳俐
Owner 深圳市阿霍烯生物科技有限公司
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