Method for extracting and separating ajoene from garlic

A technology for ajoene and garlic cloves, which is applied in the field of extracting and separating ajoene, can solve the problems of low preparation rate and low purity of ajoene, achieve strong practical value, improve yield and purity, and reduce solvent toxicity.

Pending Publication Date: 2022-08-02
QILU UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But above-mentioned two kinds of methods exist the problem that ajoene preparation rate is low, purity is not high

Method used

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  • Method for extracting and separating ajoene from garlic
  • Method for extracting and separating ajoene from garlic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for extracting and separating ajoene from garlic, comprising the following steps:

[0034] (1) Pretreated garlic: Weigh 100g garlic cloves, put them into a special ultra-high pressure bag, put them into a pressure chamber after vacuum sealing, pretreat them under 500MPa pressure for 10 minutes, and then take them out and place them at -4 to -20 Standby in ℃ environment.

[0035] (2) Pulverize the pretreated garlic cloves at a temperature of -4°C to -20°C, then put them into an ultra-high pressure chamber, and carry out an enzymatic catalytic reaction in a pressure chamber with a pressure of 100 MPa and a temperature of 25°C for 30 minutes; obtain minced garlic.

[0036] (3) Preparation of garlic oil by subcritical fluid extraction: Weigh 150 g of garlic powder into a material bag, place it in a subcritical extraction tank, and then vacuumize the extraction tank to a degree of vacuum of -0.1Mpa; add butane 600mL, the extraction temperature was 30°C, the extract...

Embodiment 2

[0040]A method for extracting and separating ajoene from garlic, comprising the following steps:

[0041] (1) Pre-treated garlic: Weigh 150g garlic cloves, put them into a special ultra-high pressure bag, put them into a pressure chamber after vacuum sealing, pre-treat them for 15 minutes under a pressure of 520MPa, and then take them out and place them at -4 to -20 Standby in ℃ environment.

[0042] (2) Pulverize the pretreated garlic cloves at a temperature of -4°C to -20°C, and then put them into an ultra-high pressure chamber, and carry out an enzyme-catalyzed reaction in a pressure chamber with a pressure of 100 MPa and a temperature of 35°C for 30 minutes to obtain minced garlic.

[0043] (3) Preparation of garlic oil by subcritical fluid extraction: Weigh 200g of garlic powder into a material bag, place it in a subcritical extraction tank, and then evacuate the extraction tank to a degree of vacuum of -0.1Mpa; add butane 900mL, the extraction temperature was 40°C, the ...

Embodiment 3

[0047] A method for extracting and separating ajoene from garlic, comprising the following steps:

[0048] (1) Pre-treated garlic: Weigh 150g garlic cloves, put them into a special ultra-high pressure bag, put them into a pressure chamber after vacuum sealing, pre-treat them for 20 minutes under a pressure of 550 MPa, and then take them out and place them at -4 to -20 Standby in ℃ environment.

[0049] (2) The pretreated garlic cloves are pulverized at a temperature of -4°C to -20°C, then placed in an ultra-high pressure chamber, and subjected to an enzymatic catalytic reaction in a pressure chamber with a pressure of 180 MPa and a temperature of 35°C for 35 minutes to obtain minced garlic.

[0050] (3) Preparation of garlic oil by subcritical fluid extraction: Weigh 200g of garlic powder into a material bag, place it in a subcritical extraction tank, and then evacuate the extraction tank to a degree of vacuum of -0.1Mpa; add butane 1200mL, the extraction temperature was 50°C...

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Abstract

The invention discloses a method for extracting and separating ajoene from garlic, and belongs to the technical field of deep processing of agricultural products. The method disclosed by the invention comprises the following steps: pre-treating garlic cloves under the conditions of vacuum, pressure of more than 400MPa and time of more than 10min; the pretreated garlic cloves are crushed at the temperature of-4 DEG C or below and then subjected to an enzymatic reaction under the conditions of 100-200 MPa and 25-35 DEG C, and minced garlic is obtained; extracting the minced garlic with subcritical fluid to obtain garlic oil; and sequentially carrying out molecular distillation and liquid phase purification on the garlic oil to obtain ajoene. According to the method, alliinase is passivated through ultrahigh pressure to extract alliin, then alliinase liquid is prepared, then alliinase in garlic is activated through ultrahigh pressure, the enzymatic reaction speed can be increased, more allicin is generated, and then ajoene with the higher content is generated; and finally, carrying out ultrahigh pressure regulation and control, subcritical fluid extraction, molecular distillation and preparative liquid phase purification to obtain a high-yield and high-purity ajoene product.

Description

technical field [0001] The invention relates to a method for extracting and separating ajoene from garlic, and belongs to the technical field of intensive and deep processing of agricultural products. Background technique [0002] Garlic contains alliin and alliinase, but alliin and alliinase exist in the cytoplasm and vacuole respectively, and alliinase cannot act on alliin. When garlic is broken, alliin is converted into allylsulfinic acid and dehydroalanine under the action of alliinase, and allylsulfinic acid generates allicin through disproportionation reaction. It is further decomposed into a diallyl sulfide compound, dithiene and ajoene. Therefore, it can be said that ajoene is the second degradation product of alliin. Under natural conditions, the amount of ajoene generated in garlic is very small, and the stability of ajoene is poor, so the extraction of ajoene is the focus of research. [0003] Chinese patent CN1324142C discloses a defatted garlic extract contain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P11/00C07C319/28C07C323/65
CPCC12P11/00C07C319/28C07C323/65Y02P20/54
Inventor 孙华于功明薛景阳庄凡静
Owner QILU UNIV OF TECH
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