A method for extracting and separating ajoene from black garlic

A technology of ajoene and black garlic, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of complex ajoene extraction process, large amount of organic reagents, and low product purity, and achieve strong Practical value, low solvent toxicity, high purity effect

Active Publication Date: 2018-12-04
SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of complex ajoene extraction process, large amount of organic reagents, low product purity, and low yield in the above technical background, the present invention provides an ajoene with high transfer rate, high purity, simple process, low solvent toxicity and Recycling and convenient extraction method

Method used

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  • A method for extracting and separating ajoene from black garlic
  • A method for extracting and separating ajoene from black garlic
  • A method for extracting and separating ajoene from black garlic

Examples

Experimental program
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Effect test

Embodiment 1

[0026] A method for extracting and isolating ajoene from black garlic, comprising the following steps:

[0027] (1) Medicinal material pulverization: take 2Kg black garlic, pulverizer pulverizes to 80 purpose garlic grains;

[0028] (2) Supercritical CO 2 Extraction: black garlic granules loaded to supercritical CO 2 In the extraction kettle of the extraction device, heat up to 40°C, increase the pressure to 15MPa, start circular extraction, keep constant temperature and pressure, extract for 0.5h, add 0.5Kg rapeseed oil continuously during the extraction process, and obtain garlic extract after the extraction is completed;

[0029] (3) Molecular distillation separation: garlic supercritical CO 2 Put the extract into the constant temperature tank of the molecular distillation device, raise the temperature of the oil heater to 60°C, control the vacuum to 30Pa, and the dropping speed is 1 drop / 3 seconds, start the distillation process, and collect the ajoene fraction;

[0030...

Embodiment 2

[0032] A method for extracting and isolating ajoene from black garlic, comprising the following steps:

[0033] (1) Medicinal material pulverization: take 2Kg black garlic, pulverizer pulverizes to 20 purpose garlic grains;

[0034] (2) Supercritical CO 2 Extraction: black garlic granules loaded to supercritical CO 2 The extraction kettle of the extraction device is heated to 75°C, and the pressure is increased to 35MPa, and the cycle extraction is started, and the constant temperature and pressure are kept, and the extraction is carried out for 3 hours. During the extraction process, 8Kg of olive oil is continuously added, and the garlic extract is obtained after the extraction is completed;

[0035] (3) Molecular distillation separation: garlic supercritical CO 2 Put the extract into the constant temperature tank of the molecular distillation device, raise the temperature of the oil heater to 120°C, control the vacuum degree to 0.1Pa, and the drop rate is 6 drops / second, s...

Embodiment 3

[0038] A method for extracting and isolating ajoene from black garlic, comprising the following steps:

[0039] (1) Medicinal material pulverization: take 3Kg black garlic, pulverizer pulverizes to 40 purpose garlic grains;

[0040] (2) Supercritical CO 2 Extraction: black garlic granules loaded to supercritical CO 2 In the extraction kettle of the extraction device, heat up to 90°C, increase the pressure to 40MPa, start circular extraction, keep constant temperature and pressure, extract for 2 hours, add 3Kg camellia oil continuously during the extraction process, and obtain garlic extract after the extraction is completed;

[0041] (3) Molecular distillation separation: garlic supercritical CO 2 Put the extract into the constant temperature tank of the molecular distillation device, raise the temperature of the oil heater to 160°C, control the vacuum degree to 6Pa, and the drop rate is 2 drops / second, start the distillation process, and collect the ajoene fraction;

[004...

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Abstract

The invention relates to a method for extracting and separating ajoene from black garlic. The method comprises four steps: 1, smashing medicinal materials; 2, performing supercritical CO2 extraction; 3, performing molecular distillation separation; 4, purifying preparative liquid phase. According to the method, the black garlic is extracted by a supercritical CO2 extraction device, the extractant is separated and enriched by a molecular distillation device, and the enriched sample is dissolved by a solvent to directly perform preparative liquid phase purification; the ajoene purity in a finished product prepared by the method is larger than 70%, and the transfer rate is 80% or more; the method is reasonable and practicable, has the advantages of being good in repeatability and stability and is suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to the technical field of extraction and separation of traditional Chinese medicines, in particular to a method for extracting and separating ajoene from black garlic. Background technique [0002] Ajoene, also known as allicene, has two structures, cis (Z-ajoene) and trans (E-ajoene), and is a naturally occurring compound obtained from garlic. Ajoene is in the process of garlic processing, alliin is converted into allylsulfenic acid and dehydroalanine under the action of alliinase, and allylsulfenic acid generates allicin through disproportionation reaction, garlic spicy element, further disproportionated into ajoene and other sulfur-containing compounds. Black garlic is a technically processed product. The content of ajoene in black garlic is higher than that in fresh garlic. The structure of ajoene is as follows: [0003] [0004] [0005] Studies have shown that ajoene has the functions of antibacterial and anti-inflam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C319/28C07C323/65
CPCC07C319/28C07C323/65Y02P20/54
Inventor 李海池马晋芳葛发欢周丹王雪莉
Owner SUN YAT SEN UNIV
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