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Immobilized compound enzyme and method for treating alliin waste liquid by using same

A technology of compound enzyme and alliin, applied in the field of biochemistry, can solve the problems of harsh use conditions of enzymes, low purity, and difficulty in recycling enzymes, and achieve the effect of improving the purity

Inactive Publication Date: 2013-05-01
SHANDONG CHENNONG HEALTH IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the mildness of the enzyme use conditions and its own variability, the enzyme is very demanding on the environment, and it is difficult to recycle and the residual enzyme has a certain impact on the performance of the product, which greatly limits the application of the enzyme in industry.
The extraction rate of garlic polysaccharides by traditional methods for extracting garlic polysaccharides is low, generally around 50% and the purity is low, and the conditions for the use of enzymes are also very harsh, which severely limits the further development of garlic polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 5ml pH 4.5, 15mg / ml pectinase (enzyme activity: 100,000 u / g) solution and 15ml pH 4.5, 15mg / ml bromelain (enzyme activity: 100,000 u / g) solution in the cone bottle,

[0021] Wherein said pectinase solution adopts concentration to be that the acetic acid solution of 0.05mol / L and concentration are the preparation of 0.05mol / L sodium acetate solution;

[0022] Described bromelain solution adopts the acetic acid solution of 0.05mol / L and concentration to be the preparation of 0.05mol / L sodium acetate solution;

[0023] Then use a pipette to draw 45ml and 35ml of acetic acid solution with a concentration of 0.05mol / L and a sodium acetate solution with a concentration of 0.05mol / L at a pH of 4.5, respectively, and put them into the conical flask, shake slightly to mix the system evenly, and put 1.0g Swollen epoxy-based macroporous resin (references Immobiliztion of candida lipolytica lipase on macroporous beaded terpolymers with epoxy groups. Wentao Liu, Hongdong Duan,...

Embodiment 2

[0027] Take 8ml of pH 5.0, 15mg / ml pectinase (enzyme activity: 100,000 u / g) solution and 12ml of pH 5.0, 15mg / ml bromelain (enzyme activity: 100,000 u / g) solution in the cone bottle,

[0028] Wherein said pectinase solution adopts concentration to be that the acetic acid solution of 0.05mol / L and concentration are the preparation of 0.05mol / L sodium acetate solution;

[0029] Described bromelain solution adopts the acetic acid solution of 0.05mol / L and concentration to be the preparation of 0.05mol / L sodium acetate solution;

[0030]Then use a pipette to draw 42ml and 38ml of acetic acid solution with a concentration of 0.05mol / L and a sodium acetate solution with a concentration of 0.05mol / L at a pH of 5.0, respectively, and put them into the Erlenmeyer flask, shake slightly to mix the system evenly, and put 1.0g Swollen epoxy-based macroporous resin (references Immobiliztion of candida lipolytica lipase on macroporous beaded terpolymers with epoxy groups. Wentao Liu, Hongdo...

Embodiment 3

[0034] Take 10ml pH 5.5, 15mg / ml pectinase (enzyme activity: 100,000 u / g) solution and 10ml pH 5.5, 15mg / ml bromelain (enzyme activity: 100,000 u / g) solution in the cone bottle,

[0035] Wherein said pectinase solution adopts concentration to be that the acetic acid solution of 0.05mol / L and concentration are the preparation of 0.05mol / L sodium acetate solution;

[0036] Described bromelain solution adopts the acetic acid solution of 0.05mol / L and concentration to be the preparation of 0.05mol / L sodium acetate solution;

[0037] Then use a pipette to draw 45ml and 35ml of acetic acid solution with a concentration of 0.05mol / L and a sodium acetate solution with a concentration of 0.05mol / L at a pH of 5.5, respectively, and put them into the Erlenmeyer flask, shake slightly to mix the system evenly, and put 1.0g Swollen epoxy-based macroporous resin (references Immobiliztion of candida lipolytica lipase on macroporous beaded terpolymers with epoxy groups. Wentao Liu, Hongdong D...

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Abstract

The invention relates to the field of biochemistry, and relates to an immobilized compound enzyme and a method for extracting garlic polysaccharide from an alliin waste liquid by using the immobilized compound enzyme. According to the method, broken kernel enzyme, namely pectinase (enzyme activity is 100,000 u / g) and protein removing enzyme, namely bromelain (enzyme activity is 100,000 u / g) are fixed in an epoxy macroporous resin, the broken kernel enzyme can hydrolyze cellulose to break cell walls, protein polysaccharide is released, and the protein removing enzyme can perform enzymolysis on protein combined with polysaccharide. The immobilized compound enzyme is adopted for extracting the garlic polysaccharide from the alliin waste liquid, a garlic polysaccharide product is prepared by steps of filtration, 1.5-5mu m ultrafiltration membrane separation, concentration and spray drying; and by the method, the yield of the polysaccharide can be improved, the content of protein in the polysaccharide is reduced and the purity of the polysaccharide is improved.

Description

technical field [0001] The invention relates to the field of biochemistry, and relates to an immobilized compound enzyme and a method for extracting garlic polysaccharide in alliin waste liquid by using the enzyme. Background technique [0002] Garlic is the underground bulb of Allium sativum L. in the Liliaceae family. It is a perennial herb and a medicinal and edible plant with a long history. The sugars in garlic are mainly fructans, which account for about 75% of the dry weight. They are the energy storage substances of garlic seeds and provide the carbon source and energy needed for garlic to germinate. Among them, the inulin content is 15-25%. In addition, garlic also contains a small amount of small molecule sugars, such as glucose, fructose and sucrose. Fructose-oligosaccharides account for about 3.9% of the total sugars in garlic, among which kestose is the basis of fructan. The total content of fructo-oligosaccharides and fructans is as high as 12.5-23.5% (wet we...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08C08B37/00
Inventor 秦大伟薛乃峰于功明赵保翠刘福锦孟霞刘先华崔波齐慧敏
Owner SHANDONG CHENNONG HEALTH IND
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