Method for extracting alliin from garlic

A technology of alliin and garlic, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of many impurities and low purity of alliin, achieve low operating temperature, convenient operation, and simple process Effect

Active Publication Date: 2011-11-23
北京晨光同创医药研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the disadvantages of low purity and many impurities in the alliin extracted in the prior art, and provide a method for extracting alliin from garlic with high purity obtained by purification with nanofiltration membrane

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for extracting alliin from garlic, characterized in that it comprises the following steps:

[0022] (1) Raw material treatment: inactivate 100g of peeled fresh garlic in a water bath at 70°C for 20 minutes, take it out and grind it in a grinder after inactivating the enzyme;

[0023] (2) According to the ratio of 1g: 5ml of the feed liquid, add the minced garlic to water, extract at 40°C for 2 hours, extract twice, and filter to obtain the filtrate;

[0024] (3) Add calcium oxide aqueous solution to the filtrate to make the pH value 10.5, incubate at 50° C. for 15 min, centrifuge, and separate to obtain the supernatant;

[0025] (4) Add phosphoric acid aqueous solution to the supernatant to make the pH value 7, incubate at 40° C. for 20 min, centrifuge, separate the supernatant, and adjust the pH value to 5.0 with phosphoric acid aqueous solution;

[0026] (5) Resin purification:

[0027] Adsorption: The supernatant obtained in step (4) is adsorbed with an io...

Embodiment 2

[0032] A method for extracting alliin from garlic, characterized in that it comprises the following steps:

[0033] (1) Raw material processing: inactivate 100g of peeled fresh garlic in a water bath at 80°C for 25 minutes, take it out after inactivating the enzyme, and grind it in a grinder;

[0034] (2) According to the ratio of 1g: 10ml of the feed liquid, add the minced garlic to water, extract at 50°C for 1 hour, extract twice, and filter to obtain the filtrate;

[0035] (3) Add an aqueous calcium oxide solution to the filtrate to make the pH value 10, incubate at 55° C. for 20 min, centrifuge, and separate to obtain the supernatant;

[0036] (4) Add phosphoric acid aqueous solution to the supernatant to make the pH value 7, incubate at 50° C. for 25 min, centrifuge, separate the supernatant, and adjust the pH value to 6.0 with phosphoric acid aqueous solution;

[0037] (5) Resin purification:

[0038] Adsorption: The supernatant obtained in step (4) is adsorbed with an...

Embodiment 3

[0043] A method for extracting alliin from garlic, characterized in that it comprises the following steps:

[0044] (1) Raw material processing: inactivate 100g of peeled fresh garlic in a water bath at 60°C for 30 minutes, take it out and grind it in a grinder after inactivating the enzyme;

[0045] (2) According to the ratio of 1g: 15ml of the feed liquid, add the minced garlic to water, extract at 40°C for 0.5h, extract 3 times, and filter to obtain the filtrate;

[0046] (3) Add an aqueous calcium oxide solution to the filtrate to make the pH value 11, incubate at 60° C. for 10 min, centrifuge, and separate to obtain a supernatant;

[0047] (4) Add phosphoric acid aqueous solution to the supernatant to make the pH value 7, incubate at 30° C. for 30 min, centrifuge, separate the supernatant, and adjust the pH value to 4.0 with phosphoric acid aqueous solution;

[0048] (5) Resin purification:

[0049] Adsorption: The supernatant obtained in step (4) is adsorbed by an ion ...

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PUM

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Abstract

The invention discloses a method for extracting alliin from garlic, which is characterized by comprising the following steps of: (1) processing a raw material, namely deactivating enzyme and grinding peeled fresh garlic; (2) mixing with water, leaching and filtering to obtain filtrate; (3) regulating the pH value, keeping the temperature, and separating to obtain supernate; (4) making the pH value 7, separating by keeping the temperature to obtain the supernate, and regulating the pH value; (5) adsorbing the supernate by using an ion exchange resin column, resolving by using ammonia water, and collecting resolving liquor; (6) regulating the pH of the resolving liquor, and making the resolving liquor pass through a nanofiltration membrane with the pore size of 150Da to obtain filtrate; and(7) concentrating and drying the filtrate to obtain the alliin. The product prepared by the method has the alliin content of 85 to 95 mass percent; and the method has the advantages of energy conservation, high efficiency, low operating temperature, simple process, convenience in operation, light pollution and the like.

Description

technical field [0001] The invention relates to a method for extracting alliin from garlic. Background technique [0002] Alliin, Ginkgo biloba extract, and ginsenoside are known as the three most important and most promising herbal medicines in the 21st century. Modern medical research has confirmed that garlic sulfur-containing amino acids represented by alliin have unique pharmacological activities. Long-term use has remarkable effects in reducing blood fat, improving body immunity, sterilizing, inhibiting bacteria, anti-cold, anti-aging, promoting blood circulation, and preventing and fighting cancer. It plays a great role in the research and development of biomedicine, and is called "one of the greatest miraculous discoveries in the field of natural medicine in the 21st century" by the world medical community. [0003] Although my country is the world's largest producer and exporter of garlic, it is generally an export model of low-value-added products. If we can extr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C317/48C07C315/06
Inventor 张卫军王如李娜张立欣
Owner 北京晨光同创医药研究院有限公司
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