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Method for separating water-soluble dietary fiber and collagen from siraitia grosvenorii waste liquid

A dietary fiber and collagen technology, which is applied to the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of high cost of purification technology and extraction technology equipment, high technical difficulty of nano-collagen purification, and operational requirements. High-level problems, to achieve low cost, considerable economic benefits, and the effect of solving environmental pollution

Active Publication Date: 2018-11-23
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the purification technology of nano-collagen is extremely difficult, and there are high threshold requirements for the production company's technological requirements and R&D capabilities
At present, the main method to obtain nano-collagen is the extraction method, but the equipment cost of the purification technology and extraction technology in this method is high, and the operation requirements are extremely high. Since the purification technology of nano-collagen is a cutting-edge technology in the global biological field, up to now There are only a handful of companies that have prepared nano-collagen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Enzymolysis: adjust the pH value of 200kg of Luo Han Guo production waste liquid to 7, add 60g of neutral protease preparation, heat and keep warm for 1.5h at 50°C to obtain the enzymolysis solution;

[0043] (2) Ultrafiltration: filter the enzymolysis solution obtained in step (1) with an ultrafiltration membrane with a molecular weight cut-off of 10,000Da at 0.6MPa, and collect 190kg of ultrafiltration membrane permeate (organic matter content is 6.30%);

[0044] (3) Separation of water-soluble dietary fiber: 190 kg of the ultrafiltration membrane permeate obtained in step (2) was put on a Sephadex G-25 dextran gel chromatography column at a flow rate of 0.5 BV / h. Gel, the volume of the gel is 350L, the aspect ratio is 10:1) for chromatography, the effluent is collected, and concentrated under reduced pressure to a solid content of 30% at a vacuum of -0.08MPa at 70°C. 2450MHz, vacuum degree -0.09MPa, microwave drying for 2.5h to obtain 5.6kg of water-soluble dieta...

Embodiment 2

[0048] (1) Enzymolysis: Adjust the pH value of 300kg of Luo Han Guo production waste liquid to 7, add 80g of neutral protease preparation, heat and keep warm at 55°C for 1 hour to obtain an enzymatic hydrolysis solution;

[0049] (2) Ultrafiltration: Filter the enzymolysis solution obtained in step (1) with an ultrafiltration membrane with a molecular weight cut-off of 15,000 Da at 1.0 MPa, and collect 280 kg of ultrafiltration membrane permeate (organic matter content is 6.25%);

[0050] (3) Separation of water-soluble dietary fiber: Put 280kg of ultrafiltration membrane permeate obtained in step (2) on a Sephadex G-15 dextran gel chromatography column at a flow rate of 0.3BV / h gel, the volume of the gel is 800L, the aspect ratio is 8:1), and the effluent is collected and concentrated under reduced pressure to a solid content of 35% at a vacuum of -0.07MPa at 80°C. 2400MHz, vacuum degree -0.08MPa, microwave drying for 2h, to obtain 9kg of water-soluble dietary fiber;

[0051...

Embodiment 3

[0054] (1) Enzymolysis: adjust the pH value of 100kg of Luo Han Guo production waste liquid to 7, add 20g of neutral protease preparation, heat and keep warm for 2 hours at 45°C for enzymolysis to obtain an enzymolysis solution;

[0055] (2) Ultrafiltration: Filter the enzymolysis solution obtained in step (1) with an ultrafiltration membrane with a molecular weight cut-off of 25,000Da at 0.8MPa, and collect 95kg of ultrafiltration membrane permeate (organic matter content is 6.50%);

[0056] (3) Separation of water-soluble dietary fiber: 95kg of ultrafiltration membrane permeate obtained in step (2) was put on a Sephadex G-50 dextran gel chromatography column at a flow rate of 0.1BV / h. gel, the volume of the gel is 300L, the ratio of height to diameter is 5:1), and the effluent is collected and concentrated under reduced pressure to a solid content of 35% at a vacuum of -0.09MPa at 60°C. 2500MHz, vacuum degree -0.09MPa, microwave drying for 1.5h, to obtain 2.73kg of water-sol...

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Abstract

The invention discloses a method for separating water-soluble dietary fiber and collagen from siraitia grosvenorii waste liquid, comprising the following steps: (1) adjusting the pH value of siraitiagrosvenorii production waste liquid to neutrality, adding a protease preparation, and performing heating, heat preservation and enzymolysis to obtain enzymatic hydrolysate; (2) filtering with an ultrafiltration membrane, and collecting ultrafiltration membrane permeate; (3) performing chromatography using a sephadex chromatographic column, collecting effluent, concentrating under reduced pressure,and drying to obtain water-soluble dietary fiber; (4) eluting, by using an eluent, the sephadex chromatographic column after the effluent is collected, filtering the effluent through a nanofiltrationmembrane, collecting nanofiltration membrane retentate, concentrating under reduced pressure, and freeze drying to obtain nano collagen. The mass content of the siraitia grosvenorii water-soluble dietary fiber product obtained by the method of the invention is as high as 97 percent, the yield is as high as 95.1 percent, the purity of the obtained siraitia grosvenorii nano collagen product is as high as 92 percent, and the total protein recovery rate is as high as 90.0 percent; the method of the invention is strong in operability, low in cost, and suitable for industrial production.

Description

technical field [0001] The invention relates to a method for separating water-soluble dietary fiber and collagen, in particular to a method for separating water-soluble dietary fiber and collagen from Luo Han Guo waste liquid. Background technique [0002] Luo Han Guo is sweet in taste and cool in nature, and it belongs to the lung and large intestine meridian. Modern medical research has confirmed that Luo Han Guo contains a natural sweetener - mogroside, which is about 400 times sweeter than sucrose, but it does not produce calories, so it is an ideal substitute drink for people who are not suitable for sugar, such as diabetes and obesity. Therefore, in In the production of Luo Han Guo, the sweet glycosides in Luo Han Guo are mainly extracted and separated, while other components are treated as waste or wastewater, which not only causes a waste of resources, but also restricts the development of the entire Luo Han Guo industry. However, in addition to mogrosides, Luo Han ...

Claims

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Application Information

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IPC IPC(8): A23L33/22C12P21/06C07K14/78C07K1/36C07K1/34C07K1/16
CPCA23V2002/00A23L33/22C07K14/78C12P21/06A23V2250/5116
Inventor 李伟龙伟岸黄华学
Owner HUNAN HUACHENG BIOTECH
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