Method for separating water-soluble dietary fiber and collagen from siraitia grosvenorii waste liquid
A dietary fiber and collagen technology, which is applied to the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of high cost of purification technology and extraction technology equipment, high technical difficulty of nano-collagen purification, and operational requirements. High-level problems, to achieve low cost, considerable economic benefits, and the effect of solving environmental pollution
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Embodiment 1
[0042] (1) Enzymolysis: adjust the pH value of 200kg of Luo Han Guo production waste liquid to 7, add 60g of neutral protease preparation, heat and keep warm for 1.5h at 50°C to obtain the enzymolysis solution;
[0043] (2) Ultrafiltration: filter the enzymolysis solution obtained in step (1) with an ultrafiltration membrane with a molecular weight cut-off of 10,000Da at 0.6MPa, and collect 190kg of ultrafiltration membrane permeate (organic matter content is 6.30%);
[0044] (3) Separation of water-soluble dietary fiber: 190 kg of the ultrafiltration membrane permeate obtained in step (2) was put on a Sephadex G-25 dextran gel chromatography column at a flow rate of 0.5 BV / h. Gel, the volume of the gel is 350L, the aspect ratio is 10:1) for chromatography, the effluent is collected, and concentrated under reduced pressure to a solid content of 30% at a vacuum of -0.08MPa at 70°C. 2450MHz, vacuum degree -0.09MPa, microwave drying for 2.5h to obtain 5.6kg of water-soluble dieta...
Embodiment 2
[0048] (1) Enzymolysis: Adjust the pH value of 300kg of Luo Han Guo production waste liquid to 7, add 80g of neutral protease preparation, heat and keep warm at 55°C for 1 hour to obtain an enzymatic hydrolysis solution;
[0049] (2) Ultrafiltration: Filter the enzymolysis solution obtained in step (1) with an ultrafiltration membrane with a molecular weight cut-off of 15,000 Da at 1.0 MPa, and collect 280 kg of ultrafiltration membrane permeate (organic matter content is 6.25%);
[0050] (3) Separation of water-soluble dietary fiber: Put 280kg of ultrafiltration membrane permeate obtained in step (2) on a Sephadex G-15 dextran gel chromatography column at a flow rate of 0.3BV / h gel, the volume of the gel is 800L, the aspect ratio is 8:1), and the effluent is collected and concentrated under reduced pressure to a solid content of 35% at a vacuum of -0.07MPa at 80°C. 2400MHz, vacuum degree -0.08MPa, microwave drying for 2h, to obtain 9kg of water-soluble dietary fiber;
[0051...
Embodiment 3
[0054] (1) Enzymolysis: adjust the pH value of 100kg of Luo Han Guo production waste liquid to 7, add 20g of neutral protease preparation, heat and keep warm for 2 hours at 45°C for enzymolysis to obtain an enzymolysis solution;
[0055] (2) Ultrafiltration: Filter the enzymolysis solution obtained in step (1) with an ultrafiltration membrane with a molecular weight cut-off of 25,000Da at 0.8MPa, and collect 95kg of ultrafiltration membrane permeate (organic matter content is 6.50%);
[0056] (3) Separation of water-soluble dietary fiber: 95kg of ultrafiltration membrane permeate obtained in step (2) was put on a Sephadex G-50 dextran gel chromatography column at a flow rate of 0.1BV / h. gel, the volume of the gel is 300L, the ratio of height to diameter is 5:1), and the effluent is collected and concentrated under reduced pressure to a solid content of 35% at a vacuum of -0.09MPa at 60°C. 2500MHz, vacuum degree -0.09MPa, microwave drying for 1.5h, to obtain 2.73kg of water-sol...
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