Marine low temperature alpha-amylase gene engineering bacteria, recombinant enzyme and application
A technology of genetically engineered strains and amylase, which is applied in the field of bioengineering, can solve the problems of reduced efficiency of microbial decomposition of organic pollutants, etc., and achieves the effects of low acting temperature, simple preparation process and high activity
CN101691579AInactive Publication Date: 2010-04-07HUAIHAI INST OF TECH +1
Patent Information
- Authority / Receiving Office
- CN · China
- Current Assignee / Owner
- HUAIHAI INST OF TECH
- Publication Date
- 2010-04-07
- Estimated Expiration
- Not applicable · inactive patent
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Abstract
The invention belongs to the field of bioengineering, and relates to engineering bacteria constructed by recombining low temperature alpha-amylase gene on an expression vector and converting to an expression host, recombinant enzyme expressed by utilizing the engineering bacteria and application thereof in industry. The invention adopts molecular operating technology, a strain of engineering bacteria EPGS230 is constructed by extracting and stipulating of bacterial genome, amplification of target genes, construction of the expression vector and conversion of recombinant vector, the engineering bacteria is processed by steps of culturing, inducing, centrifuging, ultrasound crushing, ion exchange, affinity chromatography and the like to obtain purified recombinant low temperature alpha-amylase. The inventive preparation technique has the advantages of simple operation, high enzyme activity, and low operative temperature.
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Claims
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