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Liquid compounding enzyme for brewage, and preparation method of liquid compounding enzyme

A compound enzyme and liquid enzyme technology, which is applied in the direction of beer brewing, wort preparation, biochemical equipment and methods, etc., can solve the problems of poor enzyme stability, unsuitability for beer brewing production, human hazards, etc., and achieve diacetyl The effect of low content, improving biological stability and enzyme activity stability, and high alcohol content

Active Publication Date: 2014-10-15
湖南新鸿鹰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN103131557B discloses a compound stabilizer for enzyme preparations for liquid detergents, which relates to enzyme stabilizers and solves the problem of poor enzyme stability in existing liquid detergents, and the traditional enzyme stabilizers mainly use borax, which is potentially harmful to the human body
The above-mentioned disclosed liquid enzyme preparations and stabilizers are not suitable for beer brewing production

Method used

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  • Liquid compounding enzyme for brewage, and preparation method of liquid compounding enzyme
  • Liquid compounding enzyme for brewage, and preparation method of liquid compounding enzyme
  • Liquid compounding enzyme for brewage, and preparation method of liquid compounding enzyme

Examples

Experimental program
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Effect test

Embodiment 1

[0066] Example 1 Preparation of hop extract

[0067]The preparation method of the hop extract is as follows: put the fresh mature hops in an ultrasonic cleaning machine at 200W, 40KHz, wash for 12min, drain, freeze at -20°C for 1.5h, and immediately pulverize, the thickness of the frozen material layer is 4cm, and the pulverization particle size is 2mm , add a mixture of ethanol and propanol twice the weight of crushed hops, the mass ratio of ethanol and propanol is 3:2, adjust the pH value to 3.2 with lactic acid, microwave under the conditions of power 200W, frequency 2000Hz, temperature 30℃ Intermittent extraction for 25 minutes, and ultrasonic-assisted extraction under the condition of power 300W and frequency 35KHz; heat preservation for 2h, then microwave extraction for 18min under the condition of power 300W and frequency 2000Hz, and ultrasonic-assisted extraction under the condition of power 400W and frequency 45KHz , finally cool down to room temperature naturally, fi...

Embodiment 2

[0071] The preparation of embodiment 2 Chinese herbal medicine extract

[0072] The preparation method of the Chinese herbal medicine extract is as follows: in parts by weight, accurately weigh 65 parts of astragalus, 60 parts of angelica, 42 parts of codonopsis, 42 parts of licorice, 40 parts of houttuynia, 40 parts of divine music, 30 parts of honeysuckle, Poria cocos 25 parts, 20 parts Polygala, 20 parts fried fennel, 15 parts Atractylodes Rhizoma Atractylodes Rhizome, 15 parts Magnolia officinalis; respectively crush the above-mentioned Chinese herbal medicines to a particle size below 2 mm, then mix them uniformly in a container and add 5 times the weight of water to control the temperature Keep at 80°C for 3 hours, then lower the temperature to 50°C, add a mixed enzyme preparation of 8% of the total weight of the mixed material for enzymolysis, adjust the pH value to 6.2 with lactic acid, and perform enzymatic hydrolysis for 3 hours, and finally add 1.8 times the weight o...

Embodiment 3

[0076] A liquid compound enzyme for beer brewing, prepared from the following raw materials in parts by weight:

[0077] 40 parts of liquid enzyme preparation, 10 parts of hop extract, 8 parts of sugar, 7 parts of natural antioxidant, 6 parts of Chinese herbal medicine extract, 5 parts of antibacterial peptide, 4 parts of thickener, 4 parts of β-cyclodextrin, 3 parts of polyol 3 parts glutathione, 2 parts metal chloride, 2 parts ammonium sulfate, 0.8 parts cysteine, 0.2 parts bovine serum albumin.

[0078] The liquid enzyme preparation is uniformly mixed with mesophilic amylase, dextranase, and xylanase in a volume ratio of 3.5:1.2:0.8;

[0079] The sugar is trehalose, ganoderma lucidum polysaccharide, and glucose are evenly mixed in a mass ratio of 7:2:3;

[0080] The natural antioxidant is uniformly mixed by grape seed proanthocyanidins, rosemary extract and apricot leaf extract in a mass ratio of 5:3:2;

[0081] Described thickener is uniformly mixed by mass ratio 4:3:2:1...

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Abstract

The invention discloses liquid compounding enzyme for brewage, and a preparation method of the liquid compounding enzyme, and belongs to the technical field of liquid compounding enzyme preparation. A liquid enzyme preparation for the brewage serves as a main raw material, stabilizing agents such as sugar, polyhydric alcohol, amino acid, a thickening agent, albumin and a metal ion and substances with preservative effects such as a hop extract promoting stabilization of the enzyme preparation, a Chinese herbal medicine extracting solution and antibacterial peptide are scientifically compounded, addition of a preservative is completely substituted, reproduction and growth of a harmful microbe are restrained effectively, and the activity of the liquid compounding enzyme can be cooperatively stabilized with other components, so that the biological stability and the enzyme activity stability of the liquid compounding enzyme are improved fundamentally, and the quality guarantee period of the liquid enzyme preparation is prolonged effectively. The aerobic bacterial count after the prepared liquid compounding enzyme is stored for 12 months at a room temperature is 168-214CFU / mL, and enzyme activity loss ratios after the liquid compounding enzyme is stored for 12 months at 40 DEGC and 0 DEG C are 2-2.5% and 1-1.7% respectively.

Description

technical field [0001] The invention relates to a liquid compound enzyme, in particular to a liquid compound enzyme for beer brewing and a preparation method thereof. Background technique [0002] Liquid enzyme preparation is a raw material that is easy to use, environmentally friendly and has good biodegradability. Specific catalytic properties and mild reaction conditions have been more and more used and valued in the industry. In terms of use, liquid enzyme preparations have the following advantages: 1) Liquid enzyme preparations are dispersed in the substrate in the form of molecules or particles, with large dispersion, fast absorption, rapid enzymolysis reactions, and high enzyme activity; 2) easy separation Dosage, easy compounding and secondary processing, easy to use; 3) It is beneficial to improve the utilization rate of enzyme preparations, 4) It can be used directly without dissolution and activation; 5) High purity and low impurity content; 6) Short production p...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12N9/26C12N9/30C12N9/34C12N9/44C12N9/50C12N9/16C12N9/20C12N9/92C12N9/08C12N9/88C12C7/04
CPCC12C5/004C12C7/04C12N9/0065C12N9/16C12N9/18C12N9/20C12N9/2402C12N9/2405C12N9/2411C12N9/2414C12N9/242C12N9/2425C12N9/2428C12N9/2448C12N9/2457C12N9/246C12N9/248C12N9/2488C12N9/50C12N9/63C12N9/88C12N9/92C12N9/96C12Y111/01006C12Y301/01003C12Y301/0102C12Y301/03002C12Y302/01001C12Y302/01002C12Y302/01003C12Y302/01041C12Y302/01068C12Y302/01073C12Y302/01108C12Y304/22002C12Y304/22003C12Y401/01005C12Y503/01018
Inventor 李洪兵朱永明杨志祥高志忠蔡先红
Owner 湖南新鸿鹰生物工程有限公司
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