Microbial organic fertilizer and preparation method thereof

A technology of microbial organic fertilizer and strains, applied in the field of microbial organic fertilizer and its preparation, to achieve the effects of improving fruit quality, increasing yield, and reasonable formula

Active Publication Date: 2013-10-02
DALIAN SANKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem to be solved by the present invention is to prepare composite microbial organic fertilizers by using various bacterial strains. The characteristics of this series of bacterial strains are mainly that they are extracted from nature, have strong adaptability, good stability, and are not easy to age. Interaction, equipped with high-tech production fermentation technology to produce high-quality products

Method used

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  • Microbial organic fertilizer and preparation method thereof
  • Microbial organic fertilizer and preparation method thereof
  • Microbial organic fertilizer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: jelly-like bacillus culture

[0022] 1) Inoculation: Prepare the most suitable medium plate for the strain, carry out strict aseptic operation after sterilization, transfer from the slant to the plate, and cultivate at the optimum temperature of 30°C for 2-3 days. Solid medium: sucrose 10.0g, CaSO 4 2H 2 O0.1g, K 2 HPO 4 0.2g, CaCO 3 5.0g, MgSO 4 ·7H 2 O0.2g, NaCl0.2g, agar 14.0g, distilled water to 1.0L.

[0023] 2) Primary culture: Pick a single colony from the well-grown plate and transfer it to a 50mL Erlenmeyer flask filled with the most suitable liquid medium (strict aseptic operation), and culture at 30°C and 130r / min for 24-48h. Liquid medium: sucrose 10.0g, CaSO 4 2H 2 O0.1g, K 2 HPO 4 0.2g, CaCO 3 5.0g, MgSO 4 ·7H 2 O0.2g, NaCl0.2g, distilled water to 1.0L.

[0024] 3) Secondary fermentation: Inoculate the primary culture strains into a 500mL Erlenmeyer flask filled with liquid medium, and culture at 30°C and 130r / min for 24-48h. ...

Embodiment 2

[0025] Embodiment 2: Bacillus megaterium culture

[0026] 1) Inoculation: Prepare the most suitable medium plate for the strain, carry out strict aseptic operation after sterilization, transfer from the slant to the plate, and cultivate at the optimum temperature of 30°C for 2-3 days. Solid medium: peptone 5.0g, beef extract 3.0g, NaCl 5.0g, agar 15.0g, distilled water to 1.0L, pH7.0.

[0027] 2) Primary culture: Pick a single colony from the well-grown plate and transfer it to a 50mL Erlenmeyer flask filled with the most suitable liquid medium (strict aseptic operation), and culture at 30°C and 130r / min for 24-48h. Liquid medium: peptone 5.0g, beef extract 3.0g, NaCl 5.0g, distilled water to 1.0L, pH7.0.

[0028] 3) Secondary fermentation: Inoculate the primary culture strains into a 500mL Erlenmeyer flask filled with liquid medium, and culture at 30°C and 130r / min for 24-48h. Liquid medium: peptone 5.0g, beef extract 3.0g, NaCl 5.0g, distilled water to 1.0L, pH7.0.

Embodiment 3

[0029] Embodiment 3: Azotobacter phenococcus

[0030] 1) Inoculation: Prepare the most suitable medium plate for the strain, carry out strict aseptic operation after sterilization, transfer from the slant to the plate, and cultivate at the optimum temperature of 30°C for 2-3 days. Solid medium: yeast extract 0.5g, mannitol 20.0g, KH 2 PO 4 0.2g, K 2 HPO 4 0.8g, MgSO 4 .7H 2 O0.2g, CaSO 4 2H 2 O0.1g, FeCl 3 Trace, Na 2 MoO 4 2H 2 O trace, agar 15.0g, distilled water to 1.0L, pH7.2.

[0031] 2) Primary culture: Pick a single colony from the well-grown plate and transfer it to a 50mL Erlenmeyer flask filled with the most suitable liquid medium (strict aseptic operation), and culture at 30°C and 130r / min for 24-48h. Liquid medium: yeast extract 0.5g, mannitol 20.0g, KH 2 PO 4 0.2g, K 2 HPO 4 0.8g, MgSO 4 .7H 2 O0.2g, CaSO 4 2H 2 O0.1g, FeCl 3 Trace, Na 2 MoO 4 2H 2 O trace, distilled water to 1.0L, pH7.2.

[0032] 3) Secondary fermentation: Inoculate the p...

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Abstract

The invention provides a microbial organic fertilizer and a preparation method thereof. The preparation method comprises the steps of strain culture, compounding and liquid submerged fermentation, and belongs to an agricultural integrated production technique. 15-20% of Bacillus mucilaginosus, 15-18% of Bacillus megaterium, 15-18% of Azotobacter chroococcum, 10-13% of Saccharomyces cerevisiae, 10-13% of Candida utilis, 15-18% of Lactobacillus plantarum and 10-13% of actinomycete are respectively subjected to primary and secondary strain culture and tertiary liquid submerged fermentation until the bacterial count in the fermentation liquid reaches 10-15*10<8>/mL, thereby obtaining the microbial organic fertilizer (707(Y)). By adopting the advanced liquid submerged fermentation technique and using the strains with high stability and functionality, which are screened from the natural world over the years and have the advantages of many varieties and convenient application method, the invention can promote the plant growth, improve the fruit quality, promote prematurity, increase the yield, enhance the utilization ratio of the fertilizer, improve the ecological environment of soil, and have certain effects of disease prevention and disease resistance.

Description

technical field [0001] The present invention relates to a kind of microbial organic fertilizer and preparation method thereof, in particular, to a kind of microbial organic fertilizer rich in nutrients required for crop growth produced by effective compounding of different strains through synergy ( 707(Y)), which belongs to agricultural intensive production technology. Background technique [0002] Biological fertilizers are products containing specific living microorganisms. They are applied to agricultural production. Through the life activities of the microorganisms contained in them, they can increase the supply of plant nutrients or promote plant growth, increase yield, improve the quality of agricultural products and the agricultural ecological environment. With the development of science and technology, the traditional chemical fertilizer industry is in deep trouble. New fertilizers have a broader development space in today's agricultural field due to the adoption of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/16C05F11/08C12R1/07C12R1/11C12R1/065C12R1/25C12R1/865C12R1/72C12R1/01
Inventor 顾艳丽崔国臣
Owner DALIAN SANKE BIO ENG
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