The invention discloses a research method for restraining porcine epidemic diarrhea virus infection based on integrin ligand recognition peptides. The method comprises the following steps of S1, diluting a Vero E6 cell suspension, and placing the diluted Vero E6 cell suspension in a cell culture incubator for culturing; S2, when cell density achieves 90%, removing a culture medium, and performingmoistening; S3, adding the cells to a basic culture medium containing the integrin ligand recognition peptides, and performing hatching; S4, after the liquid is removed, adding virus maintaining liquid; S5, performing inoculating with porcine epidemic diarrhea viruses; S6, after the Vero E6 cells inoculated with the viruses are placed in the cell culture incubator for 2h, sucking liquid; S7, adding the virus maintaining liquid, and placing mixed liquid in the cell culture incubator for culturing; S8, storing cells; S9, collecting samples, and performing real-time fluorescent quantitation PCR;S10, collecting samples, and performing western blotting; and S11, counting research results. The research method can verify whether the integrin ligand recognition peptides est, com and er have the effect of resisting PEDV infection, and reference is provided for prevention and control of PED and development of antiviral drugs.