Method for synergistic growth of multiple cells

A cell and carrier technology, applied to animal cells, immobilized on/in organic carriers, etc., can solve the problems of inability to achieve scale-up and provide a three-dimensional environment for cells, and achieve the effect of easy recycling and scale-up

Active Publication Date: 2013-03-13
ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI
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Problems solved by technology

However, the biggest drawback of this technology is that it cannot provide a three-di...

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  • Method for synergistic growth of multiple cells

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Embodiment Construction

[0029] 1. To investigate the interaction between chondrocytes and bone marrow mesenchymal stem cells

[0030] 1) Femur and tibia were obtained from SD rats (4 weeks old), and the bone marrow cavity was flushed with a disposable syringe to absorb serum-free DMEM medium to obtain bone marrow cells, which were separated and purified by cell density gradient centrifugation, and used with 15% fetal bovine serum The DMEM cell culture medium was cultured and subcultured in a 37°C, 5% CO2, and saturated humidity incubator. After the third passage, cell surface markers were detected by flow cytometry to verify bone marrow mesenchymal stem cells;

[0031] 2) Obtain articular cartilage from the surface of the femur and tibial head of SD rats (4 weeks old), cut it into pieces, digest with 2g / L trypsin for 5 hours, and then add 2.5g / L II at a volume ratio of 1:10 Type collagenase digestion to obtain articular chondrocytes, with DMEM cell culture medium containing 15% fetal bovine serum, in...

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Abstract

The invention relates to a method for synergistic growth of multiple cells. The method is characterized by: using a hydrogel carrier prepared under physiological conditions to culture different cells in different carriers by means of embedding small carriers in large carriers, thus studying intercellular chemical signal transmission and interaction. The biggest advantages of the method lie in that: the carrier can contain one or over two different types of cells or tissues; non-direct contact spaces of different cells are provided; the growth of different cells or tissues in a three-dimensional environment is realized; biological effects are generated by chemical signal transmission among different cells; intercellular interaction generated effects can be investigated by crushing each carrier respectively to obtain different cells successively; and during actual application, the method is easy to realize recovery and scale-up.

Description

technical field [0001] The invention relates to a method for providing synergistic growth of various cells, and also relates to preparation materials and preparation techniques related to the method. Background technique [0002] With the development of cell biology, people are increasingly aware of the importance of the interaction between different types of cells. The study of cell-cell interaction includes several aspects: direct contact between cells produces biological effects; cells produce biological effects through extracellular matrix; cells produce biological effects through chemical signal ligands and receptors, etc. At present, the research and application of the above-mentioned effects are carried out in the open system, under the condition of direct contact and co-culture between cells. The biggest disadvantage of this method is that it is difficult to distinguish whether the interaction between cells is due to direct contact effect or pure chemical signal tra...

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Application Information

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IPC IPC(8): C12N5/07C12N11/10C12N11/04
Inventor 马小军李楠于炜婷孙广炜王为
Owner ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI
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