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Forward-scattering light signal detection and collection system of flow cell analyzer and multi-angle detection method thereof

A technology of flow cytometry and forward scattered light, which is applied in individual particle analysis, particle and sedimentation analysis, measuring devices, etc., can solve problems such as flooding signals, and achieve the effects of improving detection accuracy, saving space, and low maintenance costs

Inactive Publication Date: 2019-06-21
苏州朗如精密机械科技有限公司
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  • Application Information

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Problems solved by technology

Therefore, a large amount of stray light will be focused onto the detector along with the forward scattered light, easily swamping the signal

Method used

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  • Forward-scattering light signal detection and collection system of flow cell analyzer and multi-angle detection method thereof
  • Forward-scattering light signal detection and collection system of flow cell analyzer and multi-angle detection method thereof

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Embodiment

[0033] Such as figure 1 The forward scattered light signal detection and collection system of the flow cytometer includes a liquid flow unit 1, a light source unit 2, a first speed measurement focus imaging unit 3, a second speed measurement focus imaging unit 4 and a third measurement focus imaging unit 5 , the liquid outlet of the liquid flow unit 1 can discharge the cell suspension, the light source unit 2, the first speed measurement focus imaging unit 3, the second speed measurement focus imaging unit 4 and the third measurement focus imaging unit 5 are evenly arranged in the On the circumference of the center of the liquid flow unit 1, and the light source unit 2, the first speed measurement focus imaging unit 3, the second speed measurement focus imaging unit 4 and the third measurement focus imaging unit 5 are arranged at intervals of 90°. The cell suspension passes through the imaging detection area side by side at a constant speed, the irradiation light from the ligh...

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Abstract

The invention, which belongs to the medical inspection technology and the special multifunctional rapid detection device and technology for novel device-clinical medical physiology, biochemistry and pathological examination, discloses a forward-scattering light signal detection and collection system of a flow cell analyzer and a multi-angle detection method thereof. The system comprises a fluid flow unit, a light source unit, a first speed-measuring focusing imaging unit, a second speed-measuring focusing imaging unit, and a third speed-measuring focusing imaging unit. The light source unit, the first speed-measuring focusing imaging unit, the second speed-measuring focusing imaging unit, and the third speed-measuring focusing imaging unit are distributed at the circumference using the fluid flow unit as the center uniformly; a cell suspension in the liquid flow unit flows through an imaging detection area side by side at a constant speed; scattered light entering the cell suspension at different angles enters the first speed-measuring focusing imaging unit, the second speed-measuring focusing imaging unit, and the third speed-measuring focusing imaging unit respectively to obtainthe movement speed, a defocusing amount, a bright field, a dark field, and a fluorescence image of the cell suspension.

Description

technical field [0001] The invention belongs to the technical field of medical inspection technology and new equipment—a special multi-function rapid detection device for clinical medical physiology, biochemistry, and pathology inspection, and particularly relates to a forward scattered light signal detection and collection system of a flow cytometer. The invention also relates to a multi-angle detection method of a forward scattered light signal detection and collection system of a flow cytometer. Background technique [0002] In the flow cytometric detection system, the liquid flow system can arrange the cell particles in a line, and the shaped beam is irradiated on the cells in the capillary, and each cell will have scattered light in all directions. In flow cytometry, it mainly detects forward small-angle scattered light and 90° side scattered light. [0003] The forward scattered light and the direct light of the laser passing through the particles are in the same dire...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14
Inventor 杨爱军
Owner 苏州朗如精密机械科技有限公司
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