Method of discriminating cancer and atypical cells and cell analyzer

An atypical cell and analyzer technology, applied in individual particle analysis, particle and sedimentation analysis, biochemical equipment and methods, etc. Effect

Inactive Publication Date: 2008-03-26
SYSMEX CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, the ease of operation and the accuracy and speed of determination using this marker-based method in the identification of cancer / abnormal cells are not yet very satisfactory.

Method used

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  • Method of discriminating cancer and atypical cells and cell analyzer
  • Method of discriminating cancer and atypical cells and cell analyzer
  • Method of discriminating cancer and atypical cells and cell analyzer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0151] Cancer / abnormal cells are mixed with white blood cell mass, normal squamous epithelial cell mass, and normal squamous epithelial cell mass. Therefore, identifying single cells from cell mass is a prerequisite for distinguishing cancer / abnormal cells from other cells.

[0152] In order to measure the discrimination rate of the present invention in a simulated way, artificial particles, ie microbeads, were used to replace cells.

[0153] According to the procedure shown in Fig. 11, first about 12,000 single microbeads (latex particles) with a particle diameter of 9 μm were suspended in the reticulocyte sheath fluid (manufactured by Sysmex Co., Ltd.) to prepare a measurement sample (1). Next, about 12,000 single microbeads (latex particles) with a particle size of 5 μm that were naturally aggregated into agglomerates were suspended in reticulocyte sheath fluid (manufactured by Sysmex Co., Ltd.) to prepare measurement samples (2 ) (step S1). Each measurement sample is meas...

Embodiment 2

[0161] The following describes the case where cervical bit cells are used as clinical specimens for measurement.

[0162] First, as shown in step S1 of FIG. 11, a measurement sample is prepared in the following manner.

[0163] Clinical specimens (approximately 2×10 5 Cells / tube) were centrifuged at 10,000 rpm for 1 minute, 10% N-acetyl-L-cysteine ​​PBS solution was added to the obtained particles, and then centrifuged at 10,000 rpm for 1 minute to obtain particles from which mucus was removed.

[0164] Add Zamboni fixative solution (0.2% 2,4,6-trinitrophenol, 2% paraformaldehyde) to this granule, after reacting for 10 minutes, centrifuge with 10000rpm speed for 1 minute, remove supernatant, add enzyme reaction solution ( PBS containing 0.2% type I collagenase, 0.2% type II collagenase, and 0.1% protease (manufactured by Sigma) was reacted at 37°C for 2 minutes and 30 seconds. After the reaction, an ice-cold 1% protease inhibitor (manufactured by Sigma) in PBS was added, cen...

Embodiment 3

[0177] The above-mentioned 10 kinds of characteristic parameters are calculated according to the signal waveform of the forward scattered light obtained during the test of the second embodiment, and the identification rate of these characteristic parameters in the following three aspects is investigated:

[0178] (1) Atypical cells and white blood cell clusters

[0179] (2) Atypical cells and squamous epithelial cells

[0180] (3) Atypical cells and squamous epithelial cell clusters. The discrimination threshold is set to an optimal value.

[0181] As a result, characteristic parameters with relatively high resolution obtained from the survival rate of each cell are shown in FIGS. 18 , 19 , and 20 , respectively.

[0182] That is, as shown in Figure 18 as the single characteristic parameter suitable for the discrimination of above-mentioned (1), it is B (difference integral value ÷ peak value), M (standard second-order moment) or J (waveform undulation); (2) The single char...

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Abstract

To accurately and easily discriminate cancer and atypical cells. A method which comprises measuring multiple cells with a flow cytometer, acquiring scattered light signals of individual cells, analyzing the wave shape of each scattered light signal to thereby calculate one or more characteristic parameters and then discriminating cancer and atypical cells from among these multiple cells with the use of the characteristic parameters.

Description

Technical field: [0001] The invention relates to a method for identifying cancer / abnormal cells from cell clusters (specimens) collected from living bodies and a method for identifying particle clusters required for identification. Background technique: [0002] Cytodiagnostics are effectively utilized in medical examinations as a differential method for the early detection of cancer, especially cervical cancer. [0003] Here, the cell diagnosis of cervical cancer is to wipe the surface of the cervix with a swab or a spatula, and immediately apply the scraped cells on a glass slide to make a specimen and observe it under a microscope. The observation of cell morphology under the microscope is diagnosed by cell laboratory technicians for each specimen one by one, and the accuracy and processing speed need to be improved. [0004] In recent years, instruments that automatically check cell samples and determine whether there are cancer cells have emerged as the times require. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N21/64C12Q1/02G01N33/48
CPCG01N21/6428G01N2015/1477G01N2021/4707G01N21/51G01N15/1459G01N15/1463G01N21/645
Inventor 石坂正树井邨泰之岸和希
Owner SYSMEX CORP
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