Ultra-sensitive C reactive protein detection kit and using method thereof

Abstract

The invention belongs to the field of in vitro diagnosis kits, and particularly relates to an ultra-sensitive C reactive protein detection kit and a using method thereof. The ultra-sensitive C reactive protein detection kit comprises a calibration product, a reagent R1, enzyme conjugate working liquid R2, magnetic bead conjugate working liquid M, cleaning liquid and a chemical luminous substrate,wherein the reagent R2 contains a pyridine ingredient, and can rapidly eliminate hemocytes in whole blood; and possibility that hemocytes swallow magnetic beads is avoided effectively. By the providedultra-sensitive C reactive protein detection kit, a chemiluminescence technology is combined to immunomagnetic particles, a reaction system which is close to a homogeneous phase is provided, a one-step-method reaction mode is adopted, thus, detection sensitivity and precision are greatly improved, carboxyl magnetic beads are used as a solid carrier, target substances are rapidly and effectively combined to the magnetic beads by homogeneity of the sizes and the shapes of the solid carrier, by spherical structures of the magnetic beads, a non-specific conjugate which is related to irregularly-shaped particles can further be eliminated, and the specificity of a kit product is improved.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to View More

AI Technical Summary

Benefits of technology

This patented technical effect describes various methods for detecting biological materials called CRP (cyclic acid polymerizing proteins). These techniques include combining certain reactants together into a single unit, allowing them to combine different components within their own liquid phase while maintaining convectivity during analysis. By doing these operations faster than traditional analyte extraction processes like centrifuges, there'll be no longer any chance of contamining other parts when testing small amounts of body fluid such as saliva. Additionally, the presence of free radical compounds generated through decomposition of hemoglobin may affect accurate measurement due to reduced fluorescent intensity caused by unreacted albumen molecules. Overall, these technologies improve the quality control and reliability of diagnostic tests made up of CRP/C-RNPs.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding ways to accurately determine whether certain substances like proteins called CRP may cause harm during medical procedures due to their ability to bind specifically to cell membranes involved in various physiopathological processes including inflammatoleisis caused by bacteria invading damaged areas. Current methods require complicated sample preparations involving multiple steps before analysis takes place, making them time-consuming and expensive.

Images