84results about "Sedimentation analysis" patented technology

To provide a technique which enables accurate identification of a target portion of a biological sample to be measured regardless of a label orientation without using a large image capturing mechanism when measuring the biological sample that is stored in a labeled container and separated into multiple component regions.SOLUTION: A biological sample measurement device disclosed herein is configured to identify a midpoint of luminance values of a captured image to identify an upper surface boundary and lower surface boundary of a measurement target portion, and applies a correction amounting to a meniscus width to a position of the identified upper surface boundary.SELECTED DRAWING: Figure 1

Reading device (10) for measuring the erythrocyte sedimentation rate starting from a blood sample consisting of a drop of a few microliters, which exploits capillarity to move the sample.

A system for measuring erythrocyte sedimentation rates in blood samples is described having a support for a tube containing a blood sample, an agitating element to agitate the tube, a detection unit to perform an optical measurement on the blood sample, moving means to cause movement between the detection unit and the tube during the optical measurement, and a processing unit to process signals from the detection unit. The processing unit creates a reading curve corresponding to the absorption of radiation emitted by the detection unit as a function of the relative movement between the detection unit and the tube and defines an ideal trapezoidal curve to approximate the reading curve to generate an optimized ideal curve, and generate a value indicative of the erythrocyte sedimentation rate of the blood sample in the tube. The system also includes output means to output measurement results based on the generated values.

The visible component detection accommodating device and the detection device comprise a detection part A and a detection part E; the detection part A comprises a cavity A, the cavity A is used for accommodating a sample A, the cavity A is used for shooting a microscopic magnification image of the microscopic examination sample, and the microscopic magnification image is used for visible component analysis; the detection part E comprises a cavity E; the cavity E is used for accommodating a sample E for static layering; the hierarchical information of the sample E is used to calculate the volume of the component in the visible component. The detecting device is matched with the visible component detecting and accommodating device; comprising a microscopic amplification digital imaging assembly and a layered detection assembly, the microscopic magnification digital imaging assembly is used for shooting a microscopic magnification image of the microscopic examination sample; obtaining an image of the sample in the cavity A for visible component analysis; the layering detection assembly is used for obtaining layering information of the sample E.

The invention discloses a POCT (Point of Care Testing) hematology analyzer. In the application, the first bearing position is arranged on the sample injection module to bear the detection card for receiving the first to-be-detected sample, and the second bearing position is arranged on the first detection module to bear the detection card after receiving the first to-be-detected sample, so that the receiving and detection of the first to-be-detected sample are separately carried out at different bearing positions of different modules; the transfer module is arranged at the output end of the driving module of the preprocessing module, so that the driving module can drive the transfer module to transfer the detection card from the first bearing position to the second bearing position after driving the pipetting consumable through the loading module to transfer the first to-be-detected sample into the detection card at the first bearing position; therefore, the driving module is ingeniously used for driving the transfer module, and a driving device special for driving the transfer module is avoided, so that the overall structure of the analyzer is simpler, the production cost is reduced, and the analyzer is more compact and miniaturized in structure.

A method of analyzing a blood sample from a subject includes loading the blood sample into a single chamber; acquiring, via an imaging system, a stack of serial focal plane images of the blood sample from a plurality of fields of view of the chamber; creating a virtual three dimensional image of the blood sample from selected ones of the stacks of serial focal plane images; and analyzing the virtual three dimensional image to identify blood formed elements within the blood sample. Identifying blood formed elements within the blood sample may include identifying a type and amount of white blood cells, and / or identifying an amount of red blood cells and / or hematocrit, and / or identifying an amount of platelets. Identifying blood formed elements within the blood sample may include determining numbers and percentage by volume in the blood sample of one or more types of white blood cells.

An apparatus for diffusion or staining and determination of sedimentation rate, comprising a first group (4) arranged to sample and perform a smear test on a blood sample in a test tube, and a second group (6) arranged to sample and perform a sedimentation rate determination on a blood sample in a test tube. The apparatus comprises at least one sampling member (8) which is controllable by said first group (4) and by said second group (6) to sample a blood sample, such that the sample taken for said first group (4) is not used by said second group (6) and the sample taken for said second group (6) is not used by said first group (4), said second group (6) being equipped with a sensor (20) comprising an infrared light source (12) and an optical sensor (14), said infrared light source (12) and said optical sensor (14) being arranged substantially opposite each other around a test tube (16) connected to an output of said at least one sampling member (8), such that light emitted by said infrared light source (12) reaches said optical sensor (14) after passing through said test tube (16). The apparatus further comprises a converter (10) arranged to receive one or more light transmission measurements from the optical sensor (14) and determine a sedimentation rate.

The present invention relates to a method for acquiring an image of a biological sample in a cuvette.

A container (30) is provided with an isolation chamber (33), a collection chamber (34), a waste liquid chamber (35), a siphon flow path (33a), and a siphon flow path (34a). The isolation chamber (33) holds a specific gravity adjusting agent (L2) and blood (L1), which have been layered. The collection chamber (34) holds a centrifuged mononuclear cell layer (L4), some of the specific gravity adjusting agent (L2), and plasma (L5), and allows the mononuclear cell layer (L4) to settle in a liquid after being suspended. The waste liquid chamber (35) holds the supernatant liquid of the suspension as waste liquid. The siphon flow path (33a) connects the isolation chamber (33) and the collection chamber (34), and uses siphoning to send a liquid containing the mononuclear cell layer (L4). The siphon flow path (34a) connects the collection chamber (34) and the waste liquid chamber (35), and uses siphoning to send the waste liquid.

An apparatus for performing a complete blood count and determining a sedimentation rate, comprising a first group (4) arranged to sample a blood sample from a tube and perform a complete blood count on said sample, and a second group (6) arranged to sample a blood sample from the tube and perform a sedimentation rate determination, the apparatus comprising at least one sampling member (8) which can be controlled for measurements by the first group (4) and by the second group (6) to sample a blood sample such that a sample sampled for the first group (4) is not used by the second group (6) and a sample sampled for the second group (6) is not used by the first group (4), the second group (6) being provided with a sensor (20) having an infrared light source (12) and an optical sensor (14) arranged substantially opposite each other around a tube (16) connected to an output end of the at least one sampling member (8), such that light emitted by the infrared light source (12) reaches the optical sensor (14) after traversing the tube (16). The apparatus further comprises a converter (10) arranged to receive one or more light transmission measurements from the optical sensor (14) and determine a sedimentation velocity.

An apparatus for analysing a sample, the apparatus comprising: a centrifuge rotor having an analysis site suitable for receiving a sample holder having an elongate sample chamber, the analysis site extending between an inner end, which is at a first distance from a centre of rotation of the rotor, and an outer end, which is at a second distance from the centre of rotation of the rotor, the inner end being closer to a centre of rotation of the rotor than the outer end; a carriage having an illumination arrangement and an image sensor; and a drive arrangement operable to drive the carriage along a trajectory with respect to the rotor, the trajectory extending between a first location which is relatively close to the centre of rotation of the rotor, and a second location which is relatively far from the centre of rotation of the rotor, the trajectory extending over at least part of the radial distance between the inner and outer ends of the analysis site; wherein the carriage is operable, at a plurality of locations along the trajectory, to illuminate a part of the analysis site with the illumination arrangement, and to capture an image of at least a portion of the illuminated part of the analysis site with the image sensor.

This invention belongs to the field of DNA detection technology, specifically a holographic dynamic analysis system for blood sedimentation assays. Addressing the limitation of existing systems that cannot simultaneously acquire high-dimensional dynamic phenotypic information (such as three-dimensional cell movement trajectories and instantaneous morphology) and corresponding molecular information (such as pathogen DNA and gene characteristics obtained through DNA detection) of the blood sedimentation process, and thus achieve automated correlation and intelligent interpretation between the two, thereby restricting the system's ability to directly infer microscopic etiologies from macroscopic sedimentation phenomena, this invention proposes the following solution. It includes a sample loading module connected to a sedimentation sample pool module. Through holographic dynamic three-dimensional analysis, simultaneous nucleic acid extraction and DNA detection, and multimodal AI fusion, this invention achieves correlation analysis and intelligent diagnosis of the blood sedimentation process from macroscopic dynamics to microscopic molecular mechanisms, significantly improving the system's ability to infer etiologies and support clinical decision-making.

Apparatus and method for determining information associated with particles, such as cells, in a fluid, in which images are generated at various focal lengths / depths and various times, from which particle motion over time, and therefrom density and / or type, is determined for a plurality of individual cells, bacteria, platelets, etc.

This invention provides a means to obtain more clinically useful information when analyzing particles contained in blood samples using metachromatic positive dyes. In a particle analysis method for analyzing particles contained in blood samples, particles are stained with a metachromatic positive dye; the stained particles are irradiated with light; the intensity of a first fluorescence from the stacking component of the metachromatic positive dye and the intensity of a second fluorescence from the embedded component of the metachromatic positive dye are measured, the first and second fluorescence being emitted by each particle contained in the blood sample; the intensities of the first and second fluorescence emitted by each particle are normalized according to the size of each particle to obtain the fluorescence concentration of each of the first and second fluorescence in each particle; in a two-dimensional plot of the normalized fluorescence concentrations, each particle is clustered into multiple particle clusters, including at least two of erythrocyte clusters, platelet clusters, and nucleated cell clusters; and a histogram is created where the intensity of the first fluorescence is a histogram of the RNA amount of the class and the intensity of the second fluorescence is a histogram of the DNA amount of the class included in at least one of the multiple particle clusters.

Disclosed is a blood contamination detection apparatus. The blood contamination detection apparatus comprises a computer-based contamination determining module configured to receive: one or more baseline quantified test results on an uncontaminated blood sample obtained from a patient; one or more quantified test results for one or more analytes of a blood sample or a blood component; and data input on a type of IV fluid infusion that the patient has received that is able to affect the one or more quantified test results. The computer-based contamination determining module comprises a contamination determining routine executable on a computer and configured to compare differences between the one or more baseline quantified test results and the one or more quantified test results against preset threshold difference values and / or directions and provide a contamination output. Sample contamination detection method are provided, as are other aspects.

Embodiments of the present application disclose a sample analyzer, a signal acquisition method and a computer storage medium, so that the collected light signal does not cause adverse effects on the results of blood sedimentation analysis. Embodiments of the present application comprise: a control module controls the opening or closing of a light signal generation module, a light signal acquisition module and a heating module connected in communication therewith; the control module controls the light signal acquisition module to collect light signals outside the time period when the heating module brings interference signals; the collection of light signals can not be disturbed; the control module controls the light signal acquisition module to suspend the collection of light signals within the time period when the heating module brings interference signals, thereby avoiding the collection of disturbed light signals. Through the linkage control of the light signal acquisition module and other modules, pure and undisturbed light signals can be collected in the whole signal acquisition process, which is beneficial to the subsequent signal processing and analysis process.

The present disclosure provides impedance-based detection of nucleic acids for screening of cancer. The present disclosure is based on the use of impedance spectroscopy to detect nucleic acids in solution in the clinical concentration range, without using any labels, amplification steps, bioreceptors, surface modification or complex electrode geometries. More particularly, the present disclosure leverages the structural variations in nucleic acids of healthy and cancer patients, to classify samples as cancer-positive or cancer-negative purely based on their electrophysiochemical properties. The present disclosure also provides a simple rapid, label-free miniaturized biosensor for detection and screening of cancer.

Devices and methods of the disclosure use an intravascular catheter with deployable sealing elements to create a fluid trap around an outlet of a lymphatic duct and drain lymph passively to a collection vessel that may be pressurized to a predetermined pressure or a partial vacuum. Due to the fixed pressure, the device creates a low-pressure area at the lymphatic duct, which drains lymph passively without any mechanical pump or impeller. In certain aspects, a device includes a catheter for insertion into a vein of the venous angle of a patient, with proximal and distal sealing elements deployable to seal the vein to thereby define a fluid trap around the lymphatic outlet. A port within the fluid trap opens to a drainage lumen extending along the catheter to a collection vessel. The device may be configured such that the drainage lumen passively drains the fluid trap into the collection vessel.

Disclosed are methods, materials and devices for approximation of blood volume in a fluid, such as in a biological fluid collected during a surgical procedure. The method and devices may include the use of an RBC flocculant, for example polyDADMAC, and an approximate blood hematocrit from the type of animal blood being evaluated, as well as a calculated RBC packing ratio corresponding to the collection device being used. Also provided is a Blood Indicator Panel (BIP), comprising a series of markings calculated from an observed red blood settlement volume, the average animal blood hematocrit, and a calculated RBC packing ratio “η” value for the collection device. A collection device with a BIP is disclosed. Pediatric (about 200 ml or 250 ml size container), adult human (about 1,000 ml-1,500 ml) and veterinary (about 500 ml-2,500 ml) collection containers are also disclosed, that include a RBC flocculant, for use in approximating blood volume in a fluid.

A device for determining a sedimentation rate comprises at least one member for sampling a blood sample, and a sensor comprising an infrared light source and an optical sensor arranged substantially opposite each other around a substantially transparent portion of the sampling member so that the light emitted by the infrared light source reaches the optical sensor after having passed through said substantially transparent portion. The optical sensor is arranged so as to carry out a blank measurement. A converter is arranged so as to receive one or more light transmission measurements from the optical sensor, to calculate the ratio between the blank measurement and the one or more light transmission measurements and to return a sedimentation rate.

A cuvette 300 suitable for use in analysis of biological samples comprises a collection chamber 340 and an analysis chamber 350, wherein 1-60% (parts 310, 330) of the surface area of the analysis cham

Provided herein are methods and kits to separate and isolate small volumes of peripheral blood mononuclear cells (PBMC).

The invention provides a blood sample analyzer and a blood sample analysis method.The temperature of a temperature control assembly of the blood sample analyzer reaches a first preset threshold value, to-be-detected sample liquid in the temperature control assembly is pushed into a sheath flow assembly, and the sheath flow assembly is used for obtaining pulse signals when the to-be-detected sample liquid passes through a detection area of the sheath flow assembly; the processor is used for calculating the counting stability of the to-be-detected sample liquid based on the pulse signal and obtaining a detection result of the to-be-detected sample liquid based on the counting stability. Through the mode, the to-be-detected sample liquid of the temperature control assembly is pushed into the sheath flow assembly, and the heating temperature and the heating time of the to-be-detected sample liquid are gradually increased, so that the to-be-detected sample liquid can be gradually depolymerized at the preset temperature when cell aggregation occurs; furthermore, the processor can judge whether the to-be-detected sample liquid is subjected to cell aggregation or not based on the counting stability of the to-be-detected sample liquid and obtain a corresponding detection result, so that the judgment precision of the blood sample analyzer on the cell aggregation is improved, and the counting accuracy of the blood sample analyzer is further improved.

Systems and methods are provided for controlling the location of an interface between separated blood components within a centrifuge. An optical monitoring assembly determines the current position of the interface, with the current position and a plurality of input parameters being transmitted from a controller to a neural network. The neural network employs artificial intelligence techniques to analyze the current interface position and at least one of the input parameters to determine a modification to be made to at least one procedural parameter so as to move the interface from its current position to a target position. The neural network transmits a signal that is indicative of the modification to the controller, which implements the modification so as to move the interface toward the target position, with the modification being to an inflow rate, a centrifuge rotation rate, an outflow rate, and / or the location of the target interface position.

The invention discloses a blood precipitation and hematocrit integrated detection device for clinical examination, which comprises a device main body, a control panel is arranged on the front side of the device main body, and a touch screen is arranged on the control panel; an air inlet grille is arranged on the side surface of the device main body; the device main body is provided with a placing groove, the test plate is installed in the placing groove, and the test plate is provided with a plurality of detection channels distributed in a rectangular array; the sealing cover is in sealing connection with the placing groove, and the sealing cover covers the detection channel; the upper side of the sealing cover is connected with an upper-layer cover, the top of the inner side of the upper-layer cover is detachably provided with an ultraviolet lamp through a mounting buckle, the outer side of the upper-layer cover is provided with a power supply box, and the power supply box is electrically connected with the ultraviolet lamp. The erythrocyte sedimentation hematocrit detection device is provided with the cleaning device, the interior of the detection channel is automatically sterilized, disinfected and cleaned in an air blowing mode through cooperation of an ultraviolet lamp and air blowing, manual cleaning is not needed, operation is easy and convenient, and use is more convenient and faster.

A non-contact Widmannstatten method erythrocyte sedimentation rate detection device comprises a detection box, the detection box comprises an upper box body and a lower box body, the upper box body is located above the lower box body, the rear side and the left side of the top of the lower box body are connected to the bottom face of the upper box body through vertical plates respectively, and a space between the upper box body and the lower box body forms an erythrocyte sedimentation rate detection area; in conclusion, the device disclosed by the utility model solves some key problems in the traditional Widmannstatten erythrocyte sedimentation rate detection method, such as infection risk, low operation efficiency and high starting difficulty in a blood sample treatment process, and by introducing the concept of an automatic and closed system, the device not only improves the safety and convenience of operation, but also improves the detection accuracy of the Widmannstatten erythrocyte sedimentation rate. And the cleanness and disinfection of the experimental environment are ensured.