56 results about "Blood resource" patented technology

The invention discloses a method for extracting modified plasma protein powder and bioactive peptide for enriching blood from pig blood, comprising the following steps of: taking fresh and healthy pig blood as a raw material; carrying out anticoagulation treatment, centrifuging and separation on the fresh and healthy pig blood to obtain a blood cell and blood plasma; carrying out high-pressure cutting and wall breaking, complex enzyme hydrolysis and flashing and deodorizing process treatment on the blood cell to obtain enzymolysis liquid; carrying out rough filtration, fine filtration, condensation treatment and spray drying to obtain the bioactive peptide for enriching the blood; and carrying out modification treatment on the separated blood plasma to obtain the plasma protein powder. The invention has the following advantages that: 1. the production cost is low and no pollution to the environment is generated; 2. the average molecular weight of small peptide of the produced bioactive peptide for enriching the blood is not more than 10,000Dalton, the content of free amino acid is not more than 15.0percent and the protein of the plasma protein powder is not less than 90.0 percent; 3. the water content of the produced modified plasma protein is not more than 5.0 percent, the viscosity (5 percent solution) is not less than 2.0 mpa.s and the content of the protein is not less than 90.0 percent; and 4. the process method saves time and energy, has simple operation and fully utilizes pig blood resources.

The invention relates to a processing method for boxing duck blood, which belongs to the comprehensive utilization of food resources and the technical field of food development. Fresh duck blood is taken as a raw material, and is added with an anticoagulant; the boxed duck blood which is characterized by tender texture, uniform and tiny air holes, no great deal of water eduction, high resilience, bright color and better flavor is made after the mixture is filtered, solidified, sterilized and cooled. The processing method has simple process and is convenient for operation, and is a processing process of boxed bean curd with duck blood which is suitable for large-scale industrial production and sale. In addition, the duck blood is a biological resource with high nutritive value, has a plurality of functional ingredients which have health care effects on human body, and has broad market prospect; therefore, the processing method of the invention opens a new approach to utilize the rich duck blood resource in China more effectively.

The present invention belongs to the field of medicinal products, in the concrete, it relates to a blood packaging device based on radio-frequency identification for implementing data interaction and its system. Said invention includes a blood packaging bag, on the external package of said blood packaging bag an active label is attached for making radio-frequency identification communication in the use region. The described active label includes at least one storage zone for making reading and writing operation of said active label by utilizing radio-frequency identification reader. Said invention can be used for monitoring whole process including blood collection, transportation, storage and use, etc.

The invention discloses a preparation method of pig serum protein antioxidant peptide by adopting microbial fermentation and a product thereof. The preparation method comprises steps of: (1) carrying out micro-filtering on pig serum protein; (2) inoculating the filtered pig serum protein with bacillus subtilis bacterial suspension and carrying out fermentation; and (3) decentralizing fermentation liquor, collecting liquid supernatant, and carrying out concentrating, freezing and drying to obtain the finished product. The pig serum protein antioxidant peptide prepared by the method has higher antioxidation activity, capability of eliminating free radical and better antioxidation effect both in vitro and in vivo. The preparation method is simple for operation, and is safe and reliable compared with the method of synthesizing antioxidant; and the preparation method is applicable to industrial production and can greatly enhance the utilization rate of pig serum protein resource and raise the comprehensive utilization level of pig blood so as to effectively increase additional value of pig blood resource.

A method for preparing micro-molecular hemepeptide from duck blood includes that the duck blood which is a meat duck processing byproduct is used as a raw material, and an anti-coagulation and anti-oxidation technology, a high and low temperature changing alternation technology, a dynamic ultrahigh-pressure technology, a phased enzymatic hydrolysis technology, an enzymatic hydrolysis supernatant decoloration treatment and membrane fractionation technology, spray-drying technology and the like are integrated to develop the micro-molecular hemepeptide with physiological activity within a specific molecular weight range. The average peptide chain length of the micro-molecular hemepeptide prepared by the method ranges from 2.9 amino acid residues to 3.5 amino acid residues, the molecular weight distribution range of the micro-molecular hemepeptide is 1000-3000d, and the content of hemepeptide is higher than or equal to 35%. The method has the advantages that an additional value of the product is increased, duck blood resources are totally, effectively and comprehensively utilized, a preparation process is free of pollutant discharge, and an ecological benefit of zero environmental pollution can be achieved.

The invention discloses a process for producing micromolecule hemepeptide, heme iron and plasma proteins by using duck blood as a raw material. The micromolecule hemepeptide, the heme iron and the plasma proteins which have the physiological activity in the specific molecular weight range are researched and developed by integrating an anti-freezing and antioxidation technology, a cold and heat temperature varying alternation technology, a dynamic ultrahigh pressure technology, a phase enzymolysis technology, an enzymolysis supernate decoloration technology, a membrane fractionation technology and a spray drying technology and by using by-products, namely the duck blood obtained through processing of meat duck as the raw material. According to the micromolecule hemepeptide which is obtained by the production process, the average peptide chain length is 2.9 to 3.5 amino acid residues, the molecular weight distribution range is 1,000 to 3,000d, and the content of small peptides is more than or equal to 35 percent; according to the heme iron, the protein content is more than or equal to 80 percent, the heme content is more than or equal to 15 percent, and the iron content is 2.0 to 2.5 percent; and according to the plasma proteins, the protein content is more than or equal to 85 percent, and the solubility is more than or equal to 95 percent. By the process, the duck blood resources are utilized comprehensively and efficiently, and pollutant discharge in the production process can be avoided.

The invention relates to a method for extracting hemin and globin by the use of leech protease with animal blood as a raw material. The method comprises the following main steps of: 1, carrying out middle-temperature extraction to prepare leech protease for later use; 2, taking fresh animal blood, carrying out anticoagulation treatment, centrifuging and collecting hemoglobin cells; 3, adding one time of pure water, carrying out ultrasonic treatment and breaking cells; 4, filtering through a screen mesh, leaving a filtrate, and adjusting pH value; 5, adding leech protease for enzymatic hydrolysis; 6, adjusting pH value of an enzymatic hydrolysis liquid and carrying out centrifugal separation; and 7, washing a sediment which has undergone centrifugation, carrying out dehydrolysis and drying to obtain hemin; letting a supernatant which has undergone centrifugation pass through a microfiltration membrane to remove impurities, using a nanofiltration membrane for concentration, and carrying out dehydrolysis and drying on a concentrate to obtain globin. According to the invention, animal blood resources are fully utilized and the leech extract product is used as an enzyme preparation to produce hemin and globin with high added values. The technology is simple and easy to operate, the production cost is low, and the method is suitable for industrial production.

The invention discloses a full-automatic whole blood collection and separation device and method. The whole blood collection and separation process includes two stages, wherein one stage is before reaching the blood collection volume, and the other stage is after reaching the blood collection volume. The process before reaching the blood collection volume comprises the step 1 of collecting blood, the step 2 of separating the blood, the step 3 of collecting plasma, the step 4 of stopping blood collection, the step 5 of transfusing red blood cells, the step 6 of collecting the red blood cells, the step 7 of stopping transfusion and the step 8 of starting blood collection; the process after reaching the blood collection volume comprises the step 9 of stopping blood collection, the step 10 of supplementing red blood cells, the step 11 of separating and replacing the plasma, the step 12 of collecting the plasma, the step 13 of stopping supplementation, the step 14 of transfusing the red blood cells, the step 15 of collecting the red blood cells, the step 16 of supplementing plasma according to the calculated volume and the step 17 of stopping transfusion. Through the device and method, automation of whole blood collection and separation is achieved, separated blood components meet relevant standards, and blood resources are utilized to the maximum extent.

The invention relates to an automatic blood sucking device for blood-sucking insects, in particular to an automatic blood feeder for an insect. The blood feeder comprises a constant-temperature blood supply body, an insect collecting funnel, a valve plate, a valve rod and a locking device; the constant-temperature blood supply body comprises a constant-temperature water tank and a constant-temperature plate, wherein the bottom of the constant-temperature water tank is fixedly provided with the constant-temperature plate, the bottom of the constant-temperature plate is provided with a blood groove with a downward opening, and a biological film is fixedly covered on the blood groove; the constant-temperature plate is hermetically located in a conical cavity at the upper part of the insect collecting funnel; the valve plate capable of allowing the insect to come in and go out after being moved upwards is arranged in the insect collecting funnel below the constant-temperature plate; an insect collecting cavity is formed between the bottom surface of the constant-temperature plate and the valve plate; and a plurality of ventilation holes are formed in the valve plate. The blood feeder disclosed by the invention achieves the automatic control of the temperature in the feeding process of the blood-sucking insects, enables the feeding, fixing and releasing of the blood-sucking insects to be more convenient, more efficiency and safer, and provides safety control to researches associated with harmful organisms and diseases of the harmful organisms by the adoption of the reliable and harmless blood resources, thereby having a wider application value.

The present invention relates to biological health article, and is especially the production process of deer blood wine. The deer blood wine is prepared through enzymolysis, filtering and pasteurization. The enzymolysis is used to replace in vivo digestion process to make deer blood protein cracking into peptides and amino acids as the nutritive matter for the consumer to absorb directly, and this can reduce the consumer's load in digestion and utilize fully the deer blood resource. The enzymolysis condition is one simulation of in vivo environment to ensure the complete absorption of the nutrients components. The present invention can ensure the full absorption of protein enzymolyzing liquid other effective components.

The invention discloses soluble plasma protein powder and a preparation method thereof. The soluble plasma protein powder is a mixture prepared by uniformly mixing milk powder and plasma protein powder, wherein the milk powder accounts for 10-40% of the total mass of the soluble plasma protein, pig blood accounts for 40-84% of the total mass of the plasma protein powder, and sugar accounts for 6-20% of the total mass of the plasma protein powder; the plasma protein powder is rich in fragrance, free of blood fishy smell and good in solubility and brewing stability. The preparation method comprises the preparation and operation steps of vacuum blood collection, blood anticoagulation, step-by-step enzymolysis deodorization, membrane concentration, addition of ingredients, high-pressure homogenization, spray drying, and baking and aroma extraction. The soluble plasma protein powder is fragrant, sweet and mellow in taste, has strong milk fragrance and uniform particles, and is easy to dissolve and free of caking and bad fishy smell after being brewed. The whole set of technology is adopted to process the pig blood into the convenient and instant plasma protein powder, so that the development and utilization of blood resources are realized, the utilization value of by-products such as the pig blood is greatly improved, and the market vacancy of the food plasma protein in China is supplemented.

The invention discloses a blood bag management method based on a cloud platform. The method comprises the steps of acquiring blood bag data; storing the blood bag data in local servers; uploading theblood bag data to a cloud server for storage by the local servers; and accessing the cloud server to view the blood bag data by a client. Compared with the prior art, the local servers upload the blood bag data to the cloud server for storage, so that information sharing of the blood bag data stored in the local servers is realized; and when a patient needs blood transfusion in a hospital, a usercan access the cloud server through the client to view the blood bag data, so that the blood inventory conditions of places such as hospitals corresponding to the current one or more local servers andthe like can be known, the user does not need to contact the hospitals everywhere with time and energy to ensure accurate and timely transfer of blood resources, the convenience is brought to medicalstaffs, and the patient can be treated in time. The invention further discloses a blood bag management system based on the cloud platform.

The invention discloses separating and culturing methods and an induction medium of chicken functional macrophage. The separating and culturing methods comprise the following steps: (1) preparing the medium containing macrophage stimulating factors; (2) directly obtaining chicken marrow resource macrophage precursor cells from shank or tibia of young chickens of 12-21 days, or separating chicken blood cells from chicken lymphocyte separating liquid, thus obtaining the chicken blood resource lymphocyte precursor cells; and (3) carrying out induced differentiation by adopting the medium containing the macrophage stimulating factors to obtain the chicken macrophage. The induction medium is RPMI-1640 complete medium containing the macrophage stimulating factors. According to the invention, the separating and culturing processes of the chicken functional macrophage are established for the first time, thus the mature macrophage with the phagocytic function is obtained, and a foundation is laid for the further research on the chicken macrophage on the aspects of immunity, metabolism and regulation.

The present invention relates to a blood detection method in the concrete, it relates to a method for quickly quantitiatively detecting fatty blood by adopting micro-blood-collecting process. Said method includes the following procedures: blood-taking, sealing by using rubber cement, plasma separation, turbidimetric analysis and screening. Said method can be used for judging fatty blood before blood collection, so that it has the extensive application.

The invention provides a method for preparing restrictive thrombin which is high in purity and specific activity. The method includes the steps that an anion exchange chromatography medium is utilized to adsorb prothrombin from pig plasma, an anionic chromatography column is subjected to elution, the prothrombin is activated, and a crude solution of thrombin is obtained; ultrafiltration and concentration are conducted, and a concentrated solution of the thrombin is obtained; then a cation exchange chromatography medium is utilized to adsorb thrombin, a cationic chromatography column is subjected to elution, a thrombin solution is obtained, ultra-filtration desalination concentration is conducted, finally freeze-drying is conducted, and a finished product of the restrictive thrombin is obtained. The method has the advantages that pig blood is utilized to prepare the restrictive thrombin, and it is beneficial to fully utilize pig blood resources and reduce environmental pollution; the restrictive thrombin can be prepared at a normal temperature and under normal pressure, and equipment investment is reduced; the prepared restrictive thrombin is high in purity, can reach electrophoretic purity and can be used for conducting restriction enzyme digestion on GST fusion protein; operation is simple, only steps of centrifugation, fed sample chromatography, ultrafiltration, freeze-drying and the like are needed, and industrial production is achieved easily.

The invention discloses an information system and method for optimal scheduling and accurate use of a blood resource in a region. The system comprises a communication network module, modular Internet of Things equipment, a central data processing and storage center, a man-machine interaction terminal and an AI auxiliary system. The position information and the state information of a blood resource are managed through the modular Internet of Things equipment; and through the man-machine interaction terminal and the central data processing and storage center, existing patient information and blood storage information in a hospital are input into the AI auxiliary system, and the AI auxiliary system is combined with the whole information system to give a reasonable scheduling and using scheme. The clinical blood utilization efficiency can be remarkably improved, the blood resource shortage condition is relieved, and therefore the requirement for emergency treatment is met.

The invention discloses a device and method for separating small-capacity blood components. The device and method aim to solve the problems that currently, domestically voluntary blood donation blood components are mainly separated in a manual work mode, in the mode, the purity of the blood components acquired through separation is low, blood resources are severely wasted, labor and time are wasted, efficiency is extremely low, and blood can be further wasted due to breakage of blood bags. The device comprises a machine hemostix, a first pipeline, a first three-way connector, a combination pump clamp, a second pipeline, an anticoagulant adding device and the like, wherein the first pipeline is connected with the machine hemostix, and the second pipeline is arranged on the combination pump clamp. When the device is used for voluntary blood donation (300ml to 400ml), rapid blood collection can be achieved and the blood components of whole blood can be separated immediately, and the multiple blood components can be gathered, separated and collected at one time. In the process of use, the whole blood can be automatically gathered only through a blood collection point or a blood collection vehicle, the device is used for separating and collecting the blood components, and eventually collection bags filled with the blood components are taken down.

The invention relates to an edible plasma protein powder preparing method. The method comprises the steps of health quarantine, vacuum blood sampling, blood anticoagulation, centrifugal separation, membrane concentration, homogeneous sterilization, high-temperature spray-drying, quick packaging and radiation sterilization. According to the method, pig blood is processed into edible plasma protein powder through a whole set of technologies, and development and utilization of blood resource are realized; due to the fact that plasma protein powder is rich in multiple functional ingredients including immune globulin, amino acid is balanced, and gel property is realized. The edible plasma protein powder can serve as a good food material, the use value of the pig byproduct blood resource is increased greatly, market vacancy of edible plasma protein in China is filled, and prospects are quite broad.

The invention provides a method for establishing a trauma blood transfusion prediction model. The method comprises steps of obtaining historical trauma patient blood transfusion related data as a historical user data set; acquiring original features from the historical user data set; training the original features by using a gradient boosting decision tree algorithm to obtain corresponding combined features or reconstructed new features; and extracting a characteristic parameter set suitable for representing the blood transfusion volume of a single user from a plurality of characteristics according to the distribution characteristics, related characteristics and importance of the combined characteristics or the new characteristics to train a blood transfusion prediction model. The method is advantaged in that the mode that a doctor performs empirical blood transfusion only through descriptive test results can be achieved, the success rate of patient treatment is increased, the death rate is reduced, and blood resources are saved.

The invention relates to a hemoglobin degrading bacterium NJM4 which pertains to the field of high biotechnology. The classification name of the strain NJM4 is Bacillus pumilus with the strain accession number of CGMCC NO.2337. The strain is inoculated into a seed culture medium and cultured for 24h by 120r / min at the temperature of 37 DEG C to obtain the liquid NJM4 culture. The strain produces a transparent hemolysis cycle on a blood agar culture medium, the degradation rate of hemoglobin in a hemoglobin fermentation medium is 53.6 percent and the strain degrades the hemoglobin in a hemoglobin solution into micromolecular polypeptide. The degrading bacterium utilizes the method of microorganism fermentation to degrade the hemoglobin, is suitable for the production of high-quality protein feed and can be applied to the development and utilization of the waste blood resources of livestock and poultry slaughtering, especially can transform blood proteins into feed, therefore, not only changing trash to treasure and protecting environment, but also promoting sustainable development and having wide application prospect.

The invention discloses a method for preparing nitroso hemoglobin pigment easy to disperse and high in thermal stability, and belongs to the technical field of nitroso hemoglobin pigment preparation. The method comprises the following steps: by taking ox blood as a raw material, purifying and decontaminating so as to prepare conventional nitroso hemoglobin pigment, further by taking a kudzuvine root as a raw material, crushing with a hammer, washing, collecting a kudzuvine root starch turbid liquid, standing, collecting starch slurry, compounding with hemoglobin pigment and amino acid, performing thermal treatment, wrapping, and performing freeze-drying crushing, thereby obtaining the nitroso hemoglobin pigment easy to disperse and high in thermal stability. Practical tests show that the method disclosed by the invention is unique and novel; when nitroso hemoglobin is used as a substitute of nitrite, not only is the residual amount of nitrite in a meat product reduced and is damage to human bodies reduced, but also a livestock blood resource can be sufficiently utilized, the blood resource can be developed and utilized, the iron content is increased, low-saltpetre or saltpeter-free meat products can be made indeed, and the food security is improved.

The invention discloses a preparation method of pig serum protein antioxidant peptide by adopting microbial fermentation and a product thereof. The preparation method comprises steps of: (1) carrying out micro-filtering on pig serum protein; (2) inoculating the filtered pig serum protein with bacillus subtilis bacterial suspension and carrying out fermentation; and (3) decentralizing fermentation liquor, collecting liquid supernatant, and carrying out concentrating, freezing and drying to obtain the finished product. The pig serum protein antioxidant peptide prepared by the method has higher antioxidation activity, capability of eliminating free radical and better antioxidation effect both in vitro and in vivo. The preparation method is simple for operation, and is safe and reliable compared with the method of synthesizing antioxidant; and the preparation method is applicable to industrial production and can greatly enhance the utilization rate of pig serum protein resource and raise the comprehensive utilization level of pig blood so as to effectively increase additional value of pig blood resource.

An extracorporeal circulation machine remaining blood reinfusion device comprises a venous cannula, a venous blood return chamber, a power pump, a membrane oxygenator, a fine thrombus filter, a main artery cannula and a blood recovering machine, wherein the venous cannula, the venous blood return chamber, the power pump, the membrane oxygenator and the fine thrombus filter are sequentially connected through extracorporeal circulation pipelines, and the blood recovering machine is connected with the main artery cannula. The main artery cannula is connected with the blood recovering machine through a pressure lengthening pipe, and normal saline is connected with an inputting opening of venous blood return chamber. The device has the advantages that the device can ensure that the extracorporeal circulation pipelines are always in state capable of transferring at any time after extracorporeal circulation is completed, blood in the pipelines is timely recovered and reinfused into the body of a patient, and the machine blood reinfusion method is safe, effect and economical and saves blood resources.

The invention provides a moving anticoagulant spraying device comprising (a) a first storage container used for containing an anticoagulant, (b) a communication pump having a first water inlet port and a first water outlet port, (c) a liquid output pipe used for connecting the first storage container and the first water inlet port of the self-priming pump, (d) a spray gun connected with the first water outlet port of the self-priming pump. Through improvement for deficiencies in the prior art, the moving anticoagulant spraying device is convenient to control, is hygienic and practical, facilitates quality control and provides equipment support for further processing and utilization of blood resources.

The invention relates to the technical field of medical treatment, in particular to a device and a method for recovering residual blood when ECMO patients withdraw from a machine. The device comprisesan ECMO machine, VV-ECMO or VA-ECMO cannulas, an ECMO pipeline, a blood preservation liquid bag and 0.9% of physiological saline, wherein the blood preservation liquid bag can contain recovered blood, and 0.9% of the physiological saline flushes the blood in the ECMO pipeline into the blood preservation liquid bag. During withdrawal, on the basis of strict aseptic operation, the VV-ECMO / VA-ECMO cannulas are cut off and connected through aseptic connection pipes, a three-way valve is connected to two interfaces in front of a centrifugal pump, the three-way valve at the proximal end of the centrifugal pump is connected to 0.9% of the physiological saline, the three-way valve at the distal end of the centrifugal pump is connected to the blood preservation liquid bag to form a closed loop, and the ECMO machine is started to collect residual blood. According to the device, about 500-600ml of the residual blood in the ECMO pipeline can be recovered in time, and the recovered blood treated by the blood transfusion department can be returned to patients, so that the device is economical and practical; the device is safe and effective; the recovery is complete; the operation method is simple and convenient; and the waste of blood resources is avoided.

The invention belongs to the technical field of biology, and relates to a method for preparing anhydrous chlorhematin by utilizing yak blood. The method is characterized in that the method is carried out according to the steps of collection of erythrocyte, crushing of the erythrocyte, enzymolysis of hemoglobin and extraction of chlorhematin. According to the method disclosed by the invention, the anhydrous chlorhematin is prepared by utilizing the yak blood, so that an animal blood resource is fully and valuably utilized; the method for preparing the anhydrous chlorhematin is simple and is easy to operate, used chemical reagents are less, the cost is lower, the environment pollution is lower, and the method is convenient for industrial production.

The invention discloses a preparing method of mixed concentrated platelets, and belongs to the technical field of medical transfusion. The preparing method comprises the steps of obtaining collected whole blood with a first preset capacity; adopting a centrifugal machine for centrifuging the whole blood under a first preset condition; adopting a blood composition separator for separating plasma layers in the centrifuged whole blood into platelet-poor plasma layers and platelet-rich plasma layers; adopting the blood composition separator for separating albuginea layers in the centrifuged wholeblood; adopting the blood composition separator for transferring platelet-rich plasma into an albuginea layer bag, and obtaining platelet-rich albuginea; putting the platelet-rich albuginea at a preset temperature for storage; repeating the steps above, and obtaining multiple parts of the platelet-rich albuginea with the same blood type; under a second preset condition, obtaining concentrated platelets after centrifugation, and collecting the platelets of the same blood type into a platelet storage blood bag to obtain the mixed concentrated platelets. The preparing method has the technical advantages that the capacity of the mixed concentrated platelets is low, the platelet count can meet the using requirement, and the blood resource is saved.