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Method for extracting hemin and globin from animal blood

A technology for hemin and globin peptides, which is applied in the field of extracting hemin and globin peptides with leech protease, can solve the problems of great impact on industrial production costs and high prices, achieve high quality, reduce production costs, and process simple and easy effects

Inactive Publication Date: 2012-09-12
武汉普赛特膜技术循环利用有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high price of purchasing suitable protease preparations on the market, it has a great impact on industrial production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Take 100g of leech, wash it and dry it in the sun; chop it up; put it into 0.08% sodium citrate buffer solution, the ratio of solid to liquid is 1:5, the pH value is 5.5, the temperature is 40°C, soak for 15 minutes; centrifuge Separating and collecting the supernatant; adding ammonium sulfate to the supernatant to reach a saturation of 30%, performing fractional precipitation, and obtaining the supernatant leech protease solution for later use. Take 200ml of fresh pig blood, add anticoagulant trisodium citrate at a ratio of 0.8%, mix evenly for 10 minutes, centrifuge, remove the supernatant, and collect the lower layer of hemoglobin cells, about 100ml; then add at a ratio of 1:1 Pure water, ultrasonic treatment for 15 minutes to crush the cells; filter the crushed liquid through a 100-mesh sieve, leave the filtrate, and slowly adjust the pH value of the filtrate to 2.5 with dilute hydrochloric acid; quickly raise the temperature to 85°C, and keep it warm for 10 minutes ...

Embodiment 2

[0038]Take 2kg of leeches, wash them and dry them in the sun; chop them; put them into 0.10% sodium citrate buffer solution, the ratio of solid to liquid is 1:5, the pH value is 6.0, the temperature is 45°C, soak for 20 minutes; centrifuge Separating and collecting the supernatant; adding ammonium sulfate to the supernatant to reach a saturation of 50%, performing fractional precipitation, and obtaining the supernatant leech protease solution for later use. Take 100kg of fresh chicken blood, add anticoagulant trisodium citrate at a ratio of 1.0%, mix evenly for 10 minutes, centrifuge, remove the supernatant, and collect the lower layer of hemoglobin cells, about 50kg; then add at a ratio of 1:1 Pure water, ultrasonic treatment for 20 minutes to crush the cells; filter the crushed liquid through a 100-mesh sieve, leave the filtrate, slowly adjust the pH value of the filtrate to 3.0 with dilute hydrochloric acid; quickly raise the temperature to 95°C, and keep it warm for 15 minu...

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PUM

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Abstract

The invention relates to a method for extracting hemin and globin by the use of leech protease with animal blood as a raw material. The method comprises the following main steps of: 1, carrying out middle-temperature extraction to prepare leech protease for later use; 2, taking fresh animal blood, carrying out anticoagulation treatment, centrifuging and collecting hemoglobin cells; 3, adding one time of pure water, carrying out ultrasonic treatment and breaking cells; 4, filtering through a screen mesh, leaving a filtrate, and adjusting pH value; 5, adding leech protease for enzymatic hydrolysis; 6, adjusting pH value of an enzymatic hydrolysis liquid and carrying out centrifugal separation; and 7, washing a sediment which has undergone centrifugation, carrying out dehydrolysis and drying to obtain hemin; letting a supernatant which has undergone centrifugation pass through a microfiltration membrane to remove impurities, using a nanofiltration membrane for concentration, and carrying out dehydrolysis and drying on a concentrate to obtain globin. According to the invention, animal blood resources are fully utilized and the leech extract product is used as an enzyme preparation to produce hemin and globin with high added values. The technology is simple and easy to operate, the production cost is low, and the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a production method for intensive processing of poultry blood, and mainly relates to a method for extracting hemin and globin peptide by using leech protease with poultry blood as raw material. Background technique [0002] The blood content of livestock and poultry varies depending on the species of animal. Taking pig blood as an example, its weight is about 3% to 5% of the live weight of live pigs. The blood that can be collected after slaughtering animals accounts for about 60% to 70% of the total, and the rest stays in the liver, kidney, skin and body. Usually a pig is slaughtered, and about 2.0-3.0kg of blood can be collected. [0003] Since the 1970s, my country has gradually realized that poultry blood has high nutritional and health value, but due to many factors, its utilization is not perfect, resulting in the waste of a large number of precious livestock and poultry blood resources, and causing environmental polluti...

Claims

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Application Information

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IPC IPC(8): C07D487/22C12P21/06C12N9/50
Inventor 万端极吴正奇徐国念李猷肖诗英王雄司佳蔡君邹华蓉吴尧杨涛马超徐礼鹏廖宇杰
Owner 武汉普赛特膜技术循环利用有限公司
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